Abstract
The genus Musa sp. contains commercially important fleshy fruit-producing plants, including plantains and bananas, with a strong potential for providing food security and sources of revenue to farmers. Concerns with the quality of vegetative tissues along with the possibility of the transmission of phytopathogens makes the availability of healthy plantlets limited for farmers. Micropropagation of plantains offers an alternative to producing large numbers of plantlets. However, conventional methods of micropropagation have high production costs and are labor-intensive. Recently, the temporary immersion bioreactor (TIB) has emerged as an alternative to conventional micropropagation (CM) methods. Our work utilized SEM microscopy (scanning electron microscope) and molecular and biochemical tools (qRT-PCR and ICP-OES) to characterize and compare the morphological properties, elemental composition, and photosynthetic gene expression of plantains cultured on TIB. Additionally, morphological features of growth and propagation rates were analyzed to compare outputs obtained from TIB and CM. Results showed higher growth and multiplication rates for plantlets cultivated in TIB. Gene expression analysis of selected photosynthetic genes demonstrated high transcript abundance of phosphoenolpyruvate carboxylase (PEPC) in plantain tissues obtained by TIB. Elemental composition analysis showed higher content of iron in plantains grown in TIB, suggesting a potential correlation with PEPC expression. These results demonstrate that micropropagation of Musa sp. via the liquid medium in TIB is an efficient and low-cost approach in comparison with solid media in CM.
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Claims 5
Claim · model-written summary
Plantlets cultivated in TIB showed higher growth and multiplication rates than those from conventional micropropagation.
MusaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Musa sp.”
e2“Results showed higher growth and multiplication rates for plantlets cultivated in TIB”
e3“Additionally, morphological features of growth and propagation rates were analyzed to compare outputs obtained from TIB and CM”
Claim · model-written summary
PEPC transcript abundance was high in plantain tissues obtained by TIB.
- Taxon
- plantainMusa ABB Groupe1
- Treatment
- obtained by TIBe1
- Gene
- phosphoenolpyruvate carboxylase (PEPC)e1
- Outcome
- transcript abundancee1
- Direction
- highe1
Not reportedComparator
Verbatim
e1“Gene expression analysis of selected photosynthetic genes demonstrated high transcript abundance of phosphoenolpyruvate carboxylase (PEPC) in plantain tissues obtained by TIB”
Claim · model-written summary
Plantains grown in TIB had higher iron content.
- Taxon
- plantainsMusa ABB Groupe1
- Treatment
- grown in TIBe1
- Outcome
- content of irone1
- Direction
- highere1
Not reportedComparator
Verbatim
e1“Elemental composition analysis showed higher content of iron in plantains grown in TIB”
Claim · model-written summary
The higher iron content in TIB plantains may correlate with PEPC expression.
- Taxon
- plantainsMusa ABB Groupe1
- Gene
- PEPCe2
- Outcome
- content of irone1
- Qualifier
- potential correlation with PEPC expressione2
Verbatim
e1“Elemental composition analysis showed higher content of iron in plantains grown in TIB”
e2“suggesting a potential correlation with PEPC expression”
Claim · model-written summary
Micropropagation of Musa sp. in TIB in liquid medium is an efficient and low-cost approach compared with solid media in CM.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results demonstrate that micropropagation of Musa sp. via the liquid medium in TIB is an efficient and low-cost approach in comparison with solid media in CM”
The same questions in other papers
application · pest · MusaPositive and negative results
7 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.