Abstract
This study was conducted to develop an in vitro method for the short-term conservation of the planting materials of date palm (Phoenix dactylifera). The conservation of the genetic resources of date palm species and germplasm represented by the cryopreservation of biological material, such as shoot tips of date palm dropped in LN (Liquid Nitrogen) at low temperatures, is a safe method. The main objective of this study was the collection and in vitro germplasm preservation of the date palm cultivars, and this study evaluated the in vitro preservation and genetic stability of date palm shoot tip explants using dimethyl sulfoxide (DMSO) in the medium for long-term storage. Shoot tip explants of about 2-3 cm in length were excised from in vitro cultures and were transferred to preservation media. The results showed that the highest rates of survival (80%) and recovery (75%) were observed with 1.2 M sucrose. To determine the effect of vitrification on freezing tolerances, cultures were exposed to a solution that dissolved the glaze for 60-80 minutes. The maximum survival rate obtained with exposed cultures was 85%. RAPD (randomly amplified polymorphic DNA) was used to explain the differences in the genetic characteristics of cryogenic tissue cultures and non-cryogenic tissues of date palm. Both were similar to the germinated date palm in the open field. Finally, the cryopreserved plants were able to adapt to free-living conditions after acclimatization, All aspects will contribute to the improvement of the currently available techniques for the in vitro germplasm conservation of date palms.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Survival of date palm shoot tips was highest with 1.2 M sucrose, at 80%.
- Taxon
- date palm
Phoenix dactyliferae0 - Organ / stage
- Shoot tip explantse2
- Treatment
- 1.2 M sucrosee1
- Outcome
- survivale1
- Direction
- higheste1
- Value
- 80%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“planting materials of date palm (Phoenix dactylifera)”
e1“the highest rates of survival (80%) and recovery (75%) were observed with 1.2 M sucrose”
e2“Shoot tip explants of about 2-3 cm in length were excised from in vitro cultures”
Claim · model-written summary
Recovery of date palm shoot tips was highest with 1.2 M sucrose, at 75%.
- Taxon
- date palmPhoenix dactyliferae0
- Organ / stage
- Shoot tip explantse2
- Treatment
- 1.2 M sucrosee1
- Outcome
- recoverye1
- Direction
- higheste1
- Value
- 75%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“planting materials of date palm (Phoenix dactylifera)”
e1“the highest rates of survival (80%) and recovery (75%) were observed with 1.2 M sucrose”
e2“Shoot tip explants of about 2-3 cm in length were excised from in vitro cultures”
Claim · model-written summary
Maximum survival rate of exposed date palm shoot tips was 85%.
- Taxon
- date palmPhoenix dactyliferae0
- Treatment
- a solution that dissolved the glaze for 60-80 minutese3
- Outcome
- survival ratee4
- Direction
- maximume4
- Value
- 85%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“planting materials of date palm (Phoenix dactylifera)”
e3“cultures were exposed to a solution that dissolved the glaze for 60-80 minutes”
e4“The maximum survival rate obtained with exposed cultures was 85%”
Claim · model-written summary
Genetic characteristics of cryopreserved date palm tissue cultures were similar to those of germinated date palm in the open field.
- Taxon
- date palmPhoenix dactyliferae5
- Outcome
- genetic characteristicse6
- Direction
- similare5
- Comparator
- germinated date palme5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Both were similar to the germinated date palm in the open field”
e6“was used to explain the differences in the genetic characteristics of cryogenic tissue cultures”
Claim · model-written summary
Cryopreserved date palm plants were able to adapt to free-living conditions after acclimatization.
- Taxon
- date palmPhoenix dactyliferae0
- Outcome
- adapt to free-living conditionse7
- Qualifier
- after acclimatizatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“planting materials of date palm (Phoenix dactylifera)”
e7“the cryopreserved plants were able to adapt to free-living conditions after acclimatization”
The same questions in other papers
sucrose · survival · Phoenix dactylifera (date palm)Results agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.