Abstract
Poplar is an important afforestation and urban greening species. Poplar leaf development occurs in stages, from young to mature and then from mature to senescent; these are accompanied by various phenotypic and physiological changes. However, the associated transcriptional regulatory network is relatively unexplored. We first used principal component analysis to classify poplar leaves at different leaf positions into two stages: developmental maturity (the stage of maximum photosynthetic capacity); and the stage when photosynthetic capacity started to decline and gradually changed to senescence. The two stages were then further subdivided into five intervals by gene expression clustering analysis: young leaves, the period of cell genesis and functional differentiation (L1); young leaves, the period of development and initial formation of photosynthetic capacity (L3-L7); the period of maximum photosynthetic capacity of functional leaves (L9-L13); the period of decreasing photosynthetic capacity of functional leaves (L15-L27); and the period of senescent leaves (L29). Using a weighted co-expression gene network analysis of regulatory genes, high-resolution spatiotemporal transcriptional regulatory networks were constructed to reveal the core regulators that regulate leaf development. Spatiotemporal transcriptome data of poplar leaves revealed dynamic changes in genes and miRNAs during leaf development and identified several core regulators of leaf development, such as GRF5 and MYB5. This in-depth analysis of transcriptional regulation during leaf development provides a theoretical basis for exploring the biological basis of the transcriptional regulation of leaf development and the molecular design of breeding for delaying leaf senescence.
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Claims 5
Claim · model-written summary
Poplar leaves were classified into a maturity stage with maximum photosynthetic capacity and a stage of declining capacity toward senescence.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“poplar leaves at different leaf positions into two stages: developmental maturity (the stage of maximum photosynthetic capacity); and the stage when photosynthetic capacity started to decline and gradually changed to senescence”
Claim · model-written summary
Gene expression clustering subdivided the two stages into five intervals.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two stages were then further subdivided into five intervals by gene expression clustering analysis”
t0“poplar”
Claim · model-written summary
Regulatory gene co-expression analysis constructed spatiotemporal networks for leaf development.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-resolution spatiotemporal transcriptional regulatory networks were constructed to reveal the core regulators that regulate leaf development”
t0“poplar”
Claim · model-written summary
Poplar transcriptome data showed dynamic gene and miRNA changes during leaf development.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spatiotemporal transcriptome data of poplar leaves revealed dynamic changes in genes and miRNAs during leaf development”
Claim · model-written summary
GRF5 and MYB5 were identified as core leaf development regulators.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified several core regulators of leaf development, such as GRF5 and MYB5”
t0“poplar”