Abstract
Long noncoding RNAs (lncRNAs) play important roles in various signaling pathways in vascular plants. However, the crosstalk between lncRNAs and E3 ubiquitin ligases has been barely reported. In this study, we demonstrate that the lncRNA lncWD83 from rose (Rosa chinensis) 'Old blush' activates flowering by modulating the ubiquitination of the floral repressor MYC2 LIKE (RcMYC2L). Flowering was substantially delayed in rose by virus-induced gene silencing of lncWD83. In an in vitro pull-down assay, lncWD83 associated with PLANT U-BOX PROTEIN 11 (PUB11), a U-box-containing E3 ubiquitin ligase. Seedlings with knocked down RcPUB11 transcripts phenocopied the later-flowering phenotype of lncWD83-silenced seedlings. RcMYC2L physically interacted with RcPUB11 and was ubiquitinated in an RcPUB11-dependent manner in vitro. Accordingly, silencing RcMYC2L fully reversed the later-flowering phenotype resulting from RcPUB11 knockdown. Furthermore, RcMYC2L bound to G-box-related motifs in the FLOWERING LOCUS T (RcFT) promoter and repressed its transcription. However, RcPUB11 alleviated this repression of RcFT expression via proteasomal degradation of RcMYC2L, and lncWD83 enhanced this degradation by associating with RcPUB11. Therefore, lncWD83 promotes flowering by modulating the ubiquitination of the floral repressor RcMYC2L in rose plants. These findings reveal a distinct regulatory mechanism for an lncRNA in facilitating ubiquitin-mediated proteolysis to regulate rose flowering.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Silencing lncWD83 substantially delayed rose flowering.
- Taxon
- rosee0
- Treatment
- virus-induced gene silencing of lncWD83e0
- Gene
- lncWD83e0
- Outcome
- Floweringe0
- Direction
- delayede0
Not reportedComparator
Verbatim
e0“Flowering was substantially delayed in rose by virus-induced gene silencing of lncWD83.”
Claim · model-written summary
lncWD83 associated with PUB11 in vitro.
Verbatim
e0“In an in vitro pull-down assay, lncWD83 associated with PLANT U-BOX PROTEIN 11 (PUB11), a U-box-containing E3 ubiquitin ligase.”
t0“Rose”
Claim · model-written summary
RcPUB11 knockdown produced a late-flowering phenotype like lncWD83 silencing.
- Taxon
- roset0
- Treatment
- knocked down RcPUB11 transcriptse0
- Gene
- RcPUB11e0
- Outcome
- later-flowering phenotypee0
- Comparator
- lncWD83-silenced seedlingse0
Not reportedDirection
Verbatim
e0“Seedlings with knocked down RcPUB11 transcripts phenocopied the later-flowering phenotype of lncWD83-silenced seedlings.”
t0“Rose”
Claim · model-written summary
RcMYC2L physically interacted with RcPUB11.
Verbatim
e0“RcMYC2L physically interacted with RcPUB11 and was ubiquitinated in an RcPUB11-dependent manner in vitro.”
t0“Rose”
Claim · model-written summary
RcMYC2L was ubiquitinated in an RcPUB11-dependent manner in vitro.
Verbatim
e0“RcMYC2L physically interacted with RcPUB11 and was ubiquitinated in an RcPUB11-dependent manner in vitro.”
t0“Rose”
Claim · model-written summary
Silencing RcMYC2L fully reversed the late flowering from RcPUB11 knockdown.
Verbatim
e0“silencing RcMYC2L fully reversed the later-flowering phenotype resulting from RcPUB11 knockdown.”
t0“Rose”
Claim · model-written summary
RcMYC2L bound G-box motifs in the RcFT promoter.
Verbatim
e0“RcMYC2L bound to G-box-related motifs in the FLOWERING LOCUS T (RcFT) promoter and repressed its transcription.”
t0“Rose”
Claim · model-written summary
RcMYC2L repressed RcFT transcription.
Verbatim
e0“RcMYC2L bound to G-box-related motifs in the FLOWERING LOCUS T (RcFT) promoter and repressed its transcription.”
t0“Rose”
Claim · model-written summary
RcPUB11 relieved repression of RcFT expression through proteasomal degradation of RcMYC2L.
Verbatim
e0“RcPUB11 alleviated this repression of RcFT expression via proteasomal degradation of RcMYC2L”
t0“Rose”
Claim · model-written summary
lncWD83 enhanced RcMYC2L degradation by associating with RcPUB11.
Verbatim
e0“lncWD83 enhanced this degradation by associating with RcPUB11.”
t0“Rose”
The same questions in other papers
loss of function · phenology · RosaPositive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.