Abstract
Blue honeysuckle (Lonicera caerulea L.), which belongs to the Caprifoliaceae family, is an emerging fruit crop worldwide. For the development of a transgenic system and multipurpose tissue culture, this study for the first time established an in vitro regeneration system via somatic embryogenesis, as well as improving the previously established indirect organogenesis-based regeneration system. For embryogenesis, Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) showed the highest induction rate of the embryogenic callus (97.6%), and MS supplemented with 0.1 mg/L 6-benzyladenine (6-BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 0.5 g/L activated carbon (AC) achieved the highest somatic embryo rate (28.3%). For indirect organogenesis, MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L NAA resulted in the highest non-embryogenic callus induction rate (98.9%) and adventitious shoot induction rate (51.6%). For adventitious root induction, MS supplemented with 1.0 mg/L indole-3-butyric acid (IBA) achieved the highest root induction rate (96.0%) and average root length (4.6 cm), whereas MS supplemented with 0.5 mg/L indole-3-acetic acid (IAA) resulted in the highest average regenerated root number (8.8). The total time for the regeneration from explants to soil-planted seedlings (10 euphylla) was 105 and 150 days with an efficiency of 44.1% and 23.9% through organogenesis and somatic embryogenesis, respectively. This study provides a powerful tool for rapid propagation, proliferation, and transformation, as well as laying a technological foundation for gene function research and genetic improvement of blue honeysuckle.
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Claims 11
Claim · model-written summary
MS medium with 1.0 mg/L 2,4-D gave the highest embryogenic callus induction rate, 97.6%.
- Taxon
- Blue Honeysuckle
Lonicera caeruleat0 - Treatment
- MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D)e0
- Outcome
- induction rate of the embryogenic calluse0
- Direction
- higheste0
- Value
- 97.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) showed the highest induction rate of the embryogenic callus (97.6%)”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 6-BA, NAA and activated carbon gave the highest somatic embryo rate, 28.3%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS supplemented with 0.1 mg/L 6-benzyladenine (6-BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 0.5 g/L activated carbon (AC)e0
- Outcome
- somatic embryo ratee0
- Direction
- higheste0
- Value
- 28.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS supplemented with 0.1 mg/L 6-benzyladenine (6-BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 0.5 g/L activated carbon (AC) achieved the highest somatic embryo rate (28.3%).”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 6-BA and NAA gave the highest non-embryogenic callus induction rate, 98.9%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L NAAe0
- Outcome
- non-embryogenic callus induction ratee0
- Direction
- higheste0
- Value
- 98.9%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L NAA resulted in the highest non-embryogenic callus induction rate (98.9%) and adventitious shoot induction rate (51.6%).”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 6-BA and NAA gave the highest adventitious shoot induction rate, 51.6%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L NAAe0
- Outcome
- adventitious shoot induction ratee0
- Direction
- higheste0
- Value
- 51.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.0 mg/L 6-BA and 0.1 mg/L NAA resulted in the highest non-embryogenic callus induction rate (98.9%) and adventitious shoot induction rate (51.6%).”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 1.0 mg/L IBA gave the highest root induction rate, 96.0%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS supplemented with 1.0 mg/L indole-3-butyric acid (IBA)e0
- Outcome
- root induction ratee0
- Direction
- higheste0
- Value
- 96.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS supplemented with 1.0 mg/L indole-3-butyric acid (IBA) achieved the highest root induction rate (96.0%) and average root length (4.6 cm)”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 1.0 mg/L IBA gave a highest average root length of 4.6 cm.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS supplemented with 1.0 mg/L indole-3-butyric acid (IBA)e0
- Outcome
- average root lengthe0
- Direction
- higheste0
- Value
- 4.6 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS supplemented with 1.0 mg/L indole-3-butyric acid (IBA) achieved the highest root induction rate (96.0%) and average root length (4.6 cm)”
t0“Blue Honeysuckle”
Claim · model-written summary
MS with 0.5 mg/L IAA gave the highest average regenerated root number, 8.8.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- MS supplemented with 0.5 mg/L indole-3-acetic acid (IAA)e0
- Outcome
- average regenerated root numbere0
- Direction
- higheste0
- Value
- 8.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS supplemented with 0.5 mg/L indole-3-acetic acid (IAA) resulted in the highest average regenerated root number (8.8).”
t0“Blue Honeysuckle”
Claim · model-written summary
Organogenesis produced soil-planted seedlings in 105 days.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- organogenesise0
- Outcome
- total time for the regeneration from explants to soil-planted seedlingse0
- Value
- 105e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total time for the regeneration from explants to soil-planted seedlings (10 euphylla) was 105 and 150 days with an efficiency of 44.1% and 23.9% through organogenesis and somatic embryogenesis, respectively.”
t0“Blue Honeysuckle”
Claim · model-written summary
Somatic embryogenesis produced soil-planted seedlings in 150 days.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- somatic embryogenesise0
- Outcome
- total time for the regeneration from explants to soil-planted seedlingse0
- Value
- 150 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total time for the regeneration from explants to soil-planted seedlings (10 euphylla) was 105 and 150 days with an efficiency of 44.1% and 23.9% through organogenesis and somatic embryogenesis, respectively.”
t0“Blue Honeysuckle”
Claim · model-written summary
Organogenesis regeneration efficiency was 44.1%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- organogenesise0
- Outcome
- efficiencye0
- Value
- 44.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total time for the regeneration from explants to soil-planted seedlings (10 euphylla) was 105 and 150 days with an efficiency of 44.1% and 23.9% through organogenesis and somatic embryogenesis, respectively.”
t0“Blue Honeysuckle”
Claim · model-written summary
Somatic embryogenesis regeneration efficiency was 23.9%.
- Taxon
- Blue HoneysuckleLonicera caeruleat0
- Treatment
- somatic embryogenesise0
- Outcome
- efficiencye0
- Value
- 23.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total time for the regeneration from explants to soil-planted seedlings (10 euphylla) was 105 and 150 days with an efficiency of 44.1% and 23.9% through organogenesis and somatic embryogenesis, respectively.”
t0“Blue Honeysuckle”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.