Abstract
Although the sources of molecular hydrogen (H2) synthesis in plants remain to be fully elucidated, ample evidence shows that plant-based H2 can regulate development and stress responses. Here, we present genetic and molecular evidence indicating that nitrate reductase (NR) might be a target of H2 sensing that positively regulates nitrogen use efficiency (NUE) and seed size in Arabidopsis (Arabidopsis thaliana). The expression level of NR and changes of NUE under control and, in particular, low nitrogen supply were positively associated with H2 addition supplied exogenously or through genetic manipulation. The improvement in nitrate assimilation achieved by H2 was also mediated via NR dephosphorylation. H2 control of seed size was impaired by NR mutation. Further genetic evidence revealed that H2, NR, and nitric oxide can synergistically regulate nitrate assimilation in response to N starvation conditions. Collectively, our data indicate that NR might be a target for H2 sensing, ultimately positively regulating nitrate uptake and seed size. These results provide insights into H2 signaling and its functions in plant metabolism.
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Claims 5
Claim · model-written summary
Nitrate reductase may mediate hydrogen sensing that improves nitrogen use efficiency and seed size.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Gene
- nitrate reductase (NR)e0
- Outcome
- nitrogen use efficiency (NUE) and seed sizee0
- Direction
- positively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate reductase (NR) might be a target of H2 sensing that positively regulates nitrogen use efficiency (NUE) and seed size in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Hydrogen addition or genetic manipulation was positively associated with NR expression and nitrogen use efficiency, particularly under low nitrogen.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H2 additione0
- Gene
- NRe0
- Outcome
- expression level of NR and changes of NUEe0
- Direction
- positively associatede0
- Qualifier
- low nitrogen supplye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of NR and changes of NUE under control and, in particular, low nitrogen supply were positively associated with H2 addition supplied exogenously or through genetic manipulation”
t0“seed size in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NR dephosphorylation mediated the improvement in nitrate assimilation induced by hydrogen.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NRe0
- Outcome
- nitrate assimilatione0
- Direction
- improvemente0
- Qualifier
- NR dephosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improvement in nitrate assimilation achieved by H2 was also mediated via NR dephosphorylation”
t0“seed size in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NR mutation impaired hydrogen control of seed size.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- NR mutatione0
- Gene
- NRe0
- Outcome
- H2 control of seed sizee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2 control of seed size was impaired by NR mutation”
t0“seed size in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Hydrogen, NR and nitric oxide synergistically regulated nitrate assimilation during nitrogen starvation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NRe0
- Compound
- nitric oxidee0
- Outcome
- nitrate assimilatione0
- Qualifier
- N starvation conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2, NR, and nitric oxide can synergistically regulate nitrate assimilation in response to N starvation conditions”
t0“seed size in Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
loss of function · control · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
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4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.