Abstract
Programmed cell death (PCD) facilitates selective, genetically controlled elimination of redundant, damaged, or infected cells. In plants, PCD is often an essential component of normal development and can mediate responses to abiotic and biotic stress stimuli. However, studying the transcriptional regulation of PCD is hindered by difficulties in sampling small groups of dying cells that are often buried within the bulk of living plant tissue. We addressed this challenge by using RNA sequencing and Arabidopsis thaliana suspension cells, a model system that allows precise monitoring of PCD rates. The use of three PCD-inducing treatments (salicylic acid, heat, and critical dilution), in combination with three cell death modulators (3-methyladenine, lanthanum chloride, and conditioned medium), enabled isolation of candidate core- and stimuli-specific PCD genes, inference of underlying regulatory networks and identification of putative transcriptional regulators of PCD in plants. This analysis underscored a disturbance of the cell cycle and mitochondrial retrograde signaling, and repression of pro-survival stress responses, as key elements of the PCD-associated transcriptional signature. Further, phenotyping of Arabidopsis T-DNA insertion mutants in selected candidate genes validated the potential of generated resources to identify novel genes involved in plant PCD pathways and/or stress tolerance.
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Claims 7
Claim · model-written summary
The treatment and modulator design enabled isolation of candidate core and stimulus-specific PCD genes.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- isolation of candidate core- and stimuli-specific PCD genese0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enabled isolation of candidate core- and stimuli-specific PCD genes”
t0“Arabidopsis”
Claim · model-written summary
The analysis inferred regulatory networks underlying programmed cell death.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- underlying regulatory networkse0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inference of underlying regulatory networks”
t0“Arabidopsis”
Claim · model-written summary
The analysis identified putative PCD transcriptional regulators.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- putative transcriptional regulators of PCDe0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of putative transcriptional regulators of PCD in plants”
t0“Arabidopsis”
Claim · model-written summary
Cell-cycle disturbance was a key feature of the PCD transcriptional signature.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- disturbance of the cell cyclee0
- Qualifier
- PCD-associated transcriptional signaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a disturbance of the cell cycle and mitochondrial retrograde signaling, and repression of pro-survival stress responses, as key elements of the PCD-associated transcriptional signature”
t0“Arabidopsis”
Claim · model-written summary
Mitochondrial retrograde signaling was implicated in the PCD transcriptional signature.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- mitochondrial retrograde signalinge0
- Qualifier
- PCD-associated transcriptional signaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondrial retrograde signaling, and repression of pro-survival stress responses, as key elements of the PCD-associated transcriptional signature”
t0“Arabidopsis”
Claim · model-written summary
Pro-survival stress responses were repressed in the PCD signature.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- pro-survival stress responsese0
- Direction
- repressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“repression of pro-survival stress responses, as key elements of the PCD-associated transcriptional signature”
t0“Arabidopsis”
Claim · model-written summary
Phenotyping insertion mutants validated the resource for identifying PCD and stress-tolerance genes.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Arabidopsis T-DNA insertion mutantse0
- Outcome
- identify novel genes involved in plant PCD pathways and/or stress tolerancee0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenotyping of Arabidopsis T-DNA insertion mutants in selected candidate genes validated the potential of generated resources to identify novel genes involved in plant PCD pathways and/or stress tolerance”
t0“Arabidopsis”