Abstract
The rust diseases, including leaf rust caused by Puccinia triticina ( Pt ), stem rust caused by P. graminis f. sp. tritici ( Pgt ), and stripe rust caused by P. striiformis f. sp. tritici ( Pst ), are major limiting factors in wheat production worldwide. Identification of novel sources of rust resistance genes is key to developing cultivars resistant to rapidly evolving pathogen populations. Aegilops longissima is a diploid wild grass native to the Levant and closely related to the modern bread wheat D subgenome. To explore resistance genes in the species, we evaluated a large panel of Ae. longissima for resistance to several races of Pt , Pgt , and Pst , and conducted a genome-wide association study (GWAS) to map rust resistance loci in the species. A panel of 404 Ae. longissima accessions, mostly collected from Israel, were screened for seedling-stage resistance to four races of Pt , four races of Pgt , and three races of Pst . Out of the 404 accessions screened, two were found that were resistant to all 11 races of the three rust pathogens screened. The percentage of all accessions screened that were resistant to a given rust pathogen race ranged from 18.5% to 99.7%. Genotyping-by-sequencing (GBS) was performed on 381 accessions of the Ae. longissima panel, wherein 125,343 single nucleotide polymorphisms (SNPs) were obtained after alignment to the Ae. longissima reference genome assembly and quality control filtering. Genetic diversity analysis revealed the presence of two distinct subpopulations, which followed a geographic pattern of a northern and a southern subpopulation. Association mapping was performed in the genotyped portion of the collection (n = 381) and in each subpopulation (n = 204 and 174) independently via a single-locus mixed-linear model, and two multi-locus models, FarmCPU, and BLINK. A large number (195) of markers were significantly associated with resistance to at least one of 10 rust pathogen races evaluated, nine of which are key candidate markers for further investigation due to their detection via multiple models and/or their association with resistance to more than one pathogen race. The novel resistance loci identified will provide additional diversity available for use in wheat breeding.
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Claims 7
Claim · model-written summary
Of 404 Aegilops longissima accessions screened, two were resistant to all 11 races of the three rust pathogens.
- Taxon
- Aegilops longissima
Aegilops longissimat0 - Interactor
- rust pathogense0pathogen
- Outcome
- resistant to all 11 races of the three rust pathogense0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of the 404 accessions screened, two were found that were resistant to all 11 races of the three rust pathogens screened”
t0“Aegilops longissima”
Claim · model-written summary
The percentage of accessions resistant to a given rust pathogen race ranged from 18.5% to 99.7%.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Interactor
- rust pathogene0pathogen
- Outcome
- percentage of all accessions screened that were resistante0
- Value
- 18.5% to 99.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of all accessions screened that were resistant to a given rust pathogen race ranged from 18.5% to 99.7%”
t0“Aegilops longissima”
Claim · model-written summary
Genotyping-by-sequencing of 381 accessions yielded 125,343 SNPs after alignment to the Ae. longissima reference genome and quality filtering.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Outcome
- single nucleotide polymorphisms (SNPs)e0
- Value
- 125,343e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“125,343 single nucleotide polymorphisms (SNPs) were obtained after alignment to the Ae. longissima reference genome assembly and quality control filtering”
t0“Aegilops longissima”
Claim · model-written summary
Genetic diversity analysis revealed two distinct subpopulations of Ae. longissima following a geographic pattern, northern and southern.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Outcome
- two distinct subpopulationse0
- Qualifier
- geographic pattern of a northern and a southern subpopulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic diversity analysis revealed the presence of two distinct subpopulations, which followed a geographic pattern of a northern and a southern subpopulation”
t0“Aegilops longissima”
Claim · model-written summary
A large number (195) of markers were significantly associated with resistance to at least one of 10 rust pathogen races.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Interactor
- rust pathogene0pathogen
- Gene
- markerse0
- Outcome
- resistancee0
- Value
- 195e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large number (195) of markers were significantly associated with resistance to at least one of 10 rust pathogen races evaluated”
t0“Aegilops longissima”
Claim · model-written summary
Nine of the associated markers are key candidate markers because they were detected by multiple models and/or associated with resistance to more than one pathogen race.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Gene
- key candidate markerse0
- Outcome
- resistancee0
- Value
- ninee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nine of which are key candidate markers for further investigation due to their detection via multiple models and/or their association with resistance to more than one pathogen race”
t0“Aegilops longissima”
Claim · model-written summary
The novel resistance loci identified will provide additional diversity available for use in wheat breeding.
- Taxon
- Aegilops longissimaAegilops longissimat0
- Gene
- novel resistance locie0
- Outcome
- additional diversity available for use in wheat breedinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel resistance loci identified will provide additional diversity available for use in wheat breeding”
t0“Aegilops longissima”