Abstract
Soybean plants can fix atmospheric N2 in the root nodule, a symbiotic organ with rhizobia. The primary forms of N transported from N2 fixation are ureides, allantoate, and allantoin, supplemented with asparagine. The nitrate absorbed in the roots is transported to the shoots in the forms of NO3− and asparagine with a little portion of ureides. The concentrations of N-metabolites were analyzed by capillary electrophoresis after supplying various concentrations of urea, precursors of ureides, and allopurinol, an inhibitor of xanthine dehydrogenase, to investigate the ureide synthesis pathway in the roots. When the non-nodulated soybean plants were treated with 0–5 mM of urea, the concentrations of asparagine and glutamine in the xylem sap and the roots increased remarkably. In addition, allantoate concentration increased with the urea concentrations becoming higher. Allopurinol inhibited the accumulation of allantoate but did not affect the asparagine and glutamine accumulation in roots, stems, leaves, and xylem sap, supporting that allantoate is synthesized by purine degradation in roots the same as in the nodules. When ureide precursors were supplied to the nodulated soybean plants, the concentrations of asparagine and glutamine in the xylem sap and roots increased, suggesting that the ureide precursors were absorbed and assimilated to amides in the roots.
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Claims 7
Claim · model-written summary
Urea raised asparagine and glutamine in non-nodulated soybean xylem sap and roots.
- Taxon
- soybean plants
Glycine maxe0 - Organ / stage
- xylem sap and the rootse0
- Treatment
- 0–5 mM of ureae0
- Outcome
- concentrations of asparagine and glutaminee0
- Direction
- increased remarkablye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the non-nodulated soybean plants were treated with 0–5 mM of urea, the concentrations of asparagine and glutamine in the xylem sap and the roots increased remarkably.”
Claim · model-written summary
Allantoate concentration increased with urea concentration.
- Taxon
- Soybean PlantsGlycine maxt0
- Treatment
- ureae0
- Outcome
- allantoate concentratione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allantoate concentration increased with the urea concentrations becoming higher.”
t0“Soybean Plants”
Claim · model-written summary
Allopurinol inhibited allantoate accumulation.
- Taxon
- Soybean PlantsGlycine maxt0
- Treatment
- Allopurinole0
- Outcome
- accumulation of allantoatee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Allopurinol inhibited the accumulation of allantoate but did not affect the asparagine and glutamine accumulation in roots, stems, leaves, and xylem sap”
t0“Soybean Plants”
Claim · model-written summary
Allopurinol did not affect asparagine and glutamine accumulation across plant tissues.
- Taxon
- Soybean PlantsGlycine maxt0
- Organ / stage
- roots, stems, leaves, and xylem sape0
- Treatment
- Allopurinole0
- Outcome
- asparagine and glutamine accumulatione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Allopurinol inhibited the accumulation of allantoate but did not affect the asparagine and glutamine accumulation in roots, stems, leaves, and xylem sap”
t0“Soybean Plants”
Claim · model-written summary
The authors infer allantoate is synthesized by purine degradation in roots as in nodules.
- Taxon
- Soybean PlantsGlycine maxt0
- Organ / stage
- rootse0
- Outcome
- allantoate is synthesizede0
- Qualifier
- purine degradation in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supporting that allantoate is synthesized by purine degradation in roots the same as in the nodules.”
t0“Soybean Plants”
Claim · model-written summary
Ureide precursor supply increased asparagine and glutamine in nodulated soybean xylem sap and roots.
- Taxon
- soybean plantsGlycine maxe0
- Organ / stage
- xylem sap and rootse0
- Treatment
- ureide precursorse0
- Outcome
- concentrations of asparagine and glutaminee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When ureide precursors were supplied to the nodulated soybean plants, the concentrations of asparagine and glutamine in the xylem sap and roots increased”
Claim · model-written summary
The authors infer supplied ureide precursors were absorbed and assimilated to amides in roots.
- Taxon
- Soybean PlantsGlycine maxt0
- Organ / stage
- rootse0
- Outcome
- ureide precursors were absorbed and assimilated to amidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that the ureide precursors were absorbed and assimilated to amides in the roots.”
t0“Soybean Plants”
The same questions in other papers
N-acylurea · molecular: metabolites · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
allopurinol · molecular: metabolites · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- no effect
nitrogen · molecular: metabolites · Glycine max (soybean)Results differ
36 papers in the same direction as this paper · 42 in the opposite direction · 2 reporting no difference from an alternative
- no effect
- no effect
- positive
77 papers more in this group
See all 81 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.