Abstract
Soybeans are the main sources of oil and protein for most of the global population. As the population grows, so does the demand for soybeans. However, drought is a major factor that limits soybean production. Regulating soybean response to drought stress using mepiquat chloride (MC) is a feasible method; however, its mechanism is still unclear. This study used PEG-6000 to simulate drought stress and quantitative proteomic techniques to reveal changes in Heinong44 (HN44) and Heinong65 (HN65) subjected to drought following the application of 100 mg/L of MC. The results showed that SOD in HN44 did not change significantly but decreased by 22.61% in HN65 after MC pretreatment, and MDA content decreased by 22.75% and 21.54% in HN44 and HN65, respectively. Furthermore, MC improved the GSH-ASA cycle and simultaneously promoted the Calvin cycle process to enable the plant to maintain a certain carbon assimilation rate under osmotic stress. In addition, MC upregulated some proteins during gluconeogenesis and starch metabolism and increased soluble sugar content by 8.41% in HN44. MC also reduced ribosomal protein abundance, affecting translation and amino acid metabolism. In summary, MC improved GSH-ASA cycle and Calvin cycle under stress to alleviate oxidative damage and maintain crop growth. Our study is the first to report the mechanism of MC regulation in soybean under osmotic stress, providing new insights for the rational application of MC in soybean.
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Claims 10
Claim · model-written summary
MC did not significantly change SOD in HN44.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SOD in HN44 did not change significantly”
e1“after MC pretreatment”
t0“Soybean”
Claim · model-written summary
MC decreased SOD in HN65 by 22.61%.
- Taxon
- Soybean
Glycine maxt0 - Material
- HN65e0
- Treatment
- MC pretreatmente0
- Outcome
- SODe1
- Direction
- decreasede0
- Value
- 22.61%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased by 22.61% in HN65 after MC pretreatment”
e1“SOD in HN44 did not change significantly”
t0“Soybean”
Claim · model-written summary
MC decreased MDA content in HN44 by 22.75%.
- Taxon
- SoybeanGlycine maxt0
- Material
- HN44e0
- Treatment
- MC pretreatmente1
- Outcome
- MDA contente0
- Direction
- decreasede0
- Value
- 22.75%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MDA content decreased by 22.75% and 21.54% in HN44 and HN65, respectively”
t0“Soybean”
e1“after MC pretreatment”
Claim · model-written summary
MC decreased MDA content in HN65 by 21.54%.
- Taxon
- SoybeanGlycine maxt0
- Material
- HN65e0
- Treatment
- MC pretreatmente1
- Outcome
- MDA contente0
- Direction
- decreasede0
- Value
- 21.54%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MDA content decreased by 22.75% and 21.54% in HN44 and HN65, respectively”
t0“Soybean”
e1“after MC pretreatment”
Claim · model-written summary
MC improved the GSH-ASA cycle under osmotic stress.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MCe0
- Outcome
- GSH-ASA cyclee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MC improved the GSH-ASA cycle”
t0“Soybean”
Claim · model-written summary
MC promoted the Calvin cycle under osmotic stress.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MCe0
- Outcome
- Calvin cycle processe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MC improved the GSH-ASA cycle and simultaneously promoted the Calvin cycle process”
t0“Soybean”
Claim · model-written summary
MC upregulated proteins in gluconeogenesis and starch metabolism.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MCe0
- Outcome
- proteins during gluconeogenesis and starch metabolisme0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MC upregulated some proteins during gluconeogenesis and starch metabolism”
t0“Soybean”
Claim · model-written summary
MC increased soluble sugar in HN44 by 8.41%.
- Taxon
- SoybeanGlycine maxt0
- Material
- HN44e0
- Treatment
- MCe1
- Outcome
- soluble sugar contente0
- Direction
- increasede0
- Value
- 8.41%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased soluble sugar content by 8.41% in HN44”
e1“MC also reduced ribosomal protein abundance”
t0“Soybean”
Claim · model-written summary
MC reduced ribosomal protein abundance.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MCe0
- Outcome
- ribosomal protein abundancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MC also reduced ribosomal protein abundance”
t0“Soybean”
Claim · model-written summary
The authors attribute reduced oxidative damage and maintained growth to improved GSH-ASA and Calvin cycles.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MCe0
- Outcome
- oxidative damage and maintain crop growthe0
- Qualifier
- GSH-ASA cycle and Calvin cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MC improved GSH-ASA cycle and Calvin cycle under stress to alleviate oxidative damage and maintain crop growth”
t0“Soybean”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.