Abstract
Germination under in vitro conditions for industrial hemp (Cannabis sativa L., Cannabaceae) is highly significant for the application of biotechnological tools like genome editing. Therefore, seeds were surface sterilized followed by priming with hydrogen peroxide (H2O2) and sterile distilled water (dH2O) for 24, 48, and 72 h. The primed seeds were inoculated on the Murashige and Skoog (MS) medium enriched with 1.0 mg/L benzyl amino purine (BAP) and 200 mg/L antibiotics. Exposing seeds to H2O2 was superior and 100% germination was observed. Whereas hydropriming resulted in 30-52.5% germination with a maximum of 48h priming time. The results on shoot counts revealed a maximum of 1.98 shoots from the combination of 3.0% H2O2 and 72h priming time. The results were also analyzed by constructing different statistical plots like box plots, normal plots, contour plots, and surface plots. The normal plots exhibited the significance of H2O2 concentration on both output variables. Whereas contour and surface plots classified the output data into different sub-groups and confirmed the results.
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Claims 5
Claim · model-written summary
Hydrogen peroxide priming produced 100% hemp germination and outperformed hydropriming.
- Taxon
- Cannabis sativa L.
Cannabis sativat0 - Treatment
- H2O2e0
- Outcome
- germinatione0
- Direction
- superiore0
- Value
- 100%e0
- Comparator
- hydropriminge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposing seeds to H2O2 was superior and 100% germination was observed. Whereas hydropriming resulted in 30-52.5% germination”
t0“Cannabis sativa L.”
Claim · model-written summary
Hydropriming produced 30-52.5% hemp germination, with the maximum at 48 hours.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Treatment
- hydropriminge0
- Outcome
- germinatione0
- Direction
- maximume0
- Value
- 30-52.5%e0
- Qualifier
- 48h priming timee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydropriming resulted in 30-52.5% germination with a maximum of 48h priming time”
t0“Cannabis sativa L.”
Claim · model-written summary
The combination of 3.0% H2O2 and 72h priming yielded a maximum of 1.98 shoots.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Treatment
- 3.0% H2O2 and 72h priming timee0
- Outcome
- shootse0
- Direction
- maximume0
- Value
- 1.98 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a maximum of 1.98 shoots from the combination of 3.0% H2O2 and 72h priming time”
t0“Cannabis sativa L.”
Claim · model-written summary
Normal plots found H2O2 concentration significant for both output variables.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Treatment
- H2O2 concentratione0
- Outcome
- both output variablese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The normal plots exhibited the significance of H2O2 concentration on both output variables”
t0“Cannabis sativa L.”
Claim · model-written summary
Contour and surface plots classified output data into subgroups and confirmed the results.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- classified the output data into different sub-groups and confirmed the resultse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contour and surface plots classified the output data into different sub-groups and confirmed the results”
t0“Cannabis sativa L.”
The same questions in other papers
hydrogen peroxide · growth · Cannabis sativaResults agree
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7 papers in the same direction as this paper · 1 in the opposite direction
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.