Abstract
Here, we reported the complete profiling of the crotonylation proteome in common wheat. Through a combination of crotonylation and multi-omics analysis, we identified a TaPGK associated with wheat cold stress. Then, we confirmed the positive role of TaPGK-modulating wheat cold tolerance. Meanwhile, we found that cold stress induced lysine crotonylation of TaPGK. Moreover, we screened a lysine decrotonylase TaSRT1 interacting with TaPGK and found that TaSRT1 negatively regulated wheat cold tolerance. We subsequently demonstrated TaSRT1 inhibiting the accumulation of TaPGK protein, and this inhibition was possibly resulted from decrotonylation of TaPGK by TaSRT1. Transcriptome sequencing indicated that overexpression of TaPGK activated glycolytic key genes and thereby increased pyruvate content. Moreover, we found that exogenous application of pyruvate sharply enhanced wheat cold tolerance. These findings suggest that the TaSRT1-TaPGK model regulating wheat cold tolerance is possibly through mediating pyruvate. This study provided two valuable cold tolerance genes and dissected diverse mechanism of glycolytic pathway involving in wheat cold stress.
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Claims 11
Claim · model-written summary
TaPGK was identified as associated with wheat cold stress.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaPGKe0
- Outcome
- cold stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a combination of crotonylation and multi-omics analysis, we identified a TaPGK associated with wheat cold stress.”
Claim · model-written summary
TaPGK positively modulated wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaPGKe0
- Outcome
- cold tolerancee0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Then, we confirmed the positive role of TaPGK-modulating wheat cold tolerance.”
Claim · model-written summary
Cold stress induced lysine crotonylation of TaPGK.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- cold stresse0
- Gene
- TaPGKe0
- Outcome
- lysine crotonylation of TaPGKe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Meanwhile, we found that cold stress induced lysine crotonylation of TaPGK.”
t0“wheat”
Claim · model-written summary
TaSRT1 interacted with TaPGK.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSRT1e0
- Outcome
- interacting with TaPGKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, we screened a lysine decrotonylase TaSRT1 interacting with TaPGK and found that TaSRT1 negatively regulated wheat cold tolerance.”
Claim · model-written summary
TaSRT1 negatively regulated wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSRT1e0
- Outcome
- cold tolerancee0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, we screened a lysine decrotonylase TaSRT1 interacting with TaPGK and found that TaSRT1 negatively regulated wheat cold tolerance.”
Claim · model-written summary
TaSRT1 inhibited accumulation of TaPGK protein.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaSRT1e0
- Outcome
- accumulation of TaPGK proteine0
- Direction
- inhibitinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We subsequently demonstrated TaSRT1 inhibiting the accumulation of TaPGK protein, and this inhibition was possibly resulted from decrotonylation of TaPGK by TaSRT1.”
t0“wheat”
Claim · model-written summary
The authors proposed that TaSRT1 decrotonylation of TaPGK explains inhibition of TaPGK accumulation.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaSRT1e0
- Outcome
- inhibitione0
- Qualifier
- decrotonylation of TaPGK by TaSRT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We subsequently demonstrated TaSRT1 inhibiting the accumulation of TaPGK protein, and this inhibition was possibly resulted from decrotonylation of TaPGK by TaSRT1.”
t0“wheat”
Claim · model-written summary
TaPGK overexpression activated glycolytic key genes.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaPGKe0
- Gene
- TaPGKe0
- Outcome
- glycolytic key genese0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome sequencing indicated that overexpression of TaPGK activated glycolytic key genes and thereby increased pyruvate content.”
t0“wheat”
Claim · model-written summary
TaPGK overexpression increased pyruvate content.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaPGKe0
- Gene
- TaPGKe0
- Outcome
- pyruvate contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome sequencing indicated that overexpression of TaPGK activated glycolytic key genes and thereby increased pyruvate content.”
t0“wheat”
Claim · model-written summary
Exogenous pyruvate sharply enhanced wheat cold tolerance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- exogenous application of pyruvatee0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, we found that exogenous application of pyruvate sharply enhanced wheat cold tolerance.”
Claim · model-written summary
The authors propose that TaSRT1-TaPGK regulates wheat cold tolerance through pyruvate.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSRT1-TaPGKe0
- Compound
- pyruvatee0
- Outcome
- cold tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These findings suggest that the TaSRT1-TaPGK model regulating wheat cold tolerance is possibly through mediating pyruvate.”
The same questions in other papers
pyruvate · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.