Paper · 2023 · Current Research in Nutrition and Food Science Journal

Method Optimization for Quantification of GABA and Isoflavone Aglycones in Germinated Soybean

Dinh T Tran; Huyen Thi Vu; Daylan A. Tzompa-Sosa; et al.

5
claims
8
verbatim quotes
3
citations
2
research areas
✓
Open access · cc-by

Abstract

Germinated soybean is rich in bioactive compounds such as γ-aminobutyric acid (GABA), polyphenols, and flavonoids. Thus, quantification of these components is critical for assessing nutritional value of soybean after germination and processing. In the current research, methods for determination of GABA, daidzein, genistein, and glycitein in germinated soybeans using high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV-VIS) were optimized. Then, soybean germination time was optimized in ‘DT84’ and ‘DT51’, two Vietnamese soybean cultivars. These cultivars were soaked for 1 h, 2 h, 3 h, 4 h, and 5 h with water-to-bean ratio of 1:5 at 26 - 28 ºC to initiate germination. After being soaked, the seeds were germinated, dried, and ground into powder for quantification of GABA and isoflavone aglycones. The highest concentration of GABA, daidzein and genistein were reached after 1 h of bean soaking. Further, germinated ‘DT51’ had higher GABA and lower genistein and daidzein content than germinated ‘DT84’.Previous studies had reported the extraction and quantification of GABA and isoflavone aglycones in various food matrices. However, in this study we performed optimization of these procedures in a soybean matrix and showed potential of germination as a low-cost processing to boost the nutritional quality of soybean.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 5

Associationobservedoriginal resultmeasured

Claim · model-written summary

The highest concentrations of GABA, daidzein and genistein were reached after 1 h of soaking

Taxon
soybeanGlycine maxe0
Treatment
1 h of bean soakinge1
Outcome
concentration of GABA, daidzein and genisteine1
Direction
higheste1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Germinated soybean is rich in bioactive compounds”

✓ located verbatim in the source · abstract, characters 0–49 · show in text ↑
e1

“The highest concentration of GABA, daidzein and genistein were reached after 1 h of bean soaking”

✓ located verbatim in the source · abstract, characters 834–930 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Germinated DT51 had higher GABA content than DT84

Taxon
soybeanGlycine maxe0
Material
DT51e2
Outcome
GABAe2
Direction
highere2
Comparator
DT84e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Germinated soybean is rich in bioactive compounds”

✓ located verbatim in the source · abstract, characters 0–49 · show in text ↑
e2

“germinated ‘DT51’ had higher GABA and lower genistein and daidzein content than germinated ‘DT84’”

✓ located verbatim in the source · abstract, characters 941–1038 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Germinated DT51 had lower genistein and daidzein content than DT84

Taxon
soybeanGlycine maxe0
Material
DT51e2
Outcome
genistein and daidzein contente2
Direction
lowere2
Comparator
DT84e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Germinated soybean is rich in bioactive compounds”

✓ located verbatim in the source · abstract, characters 0–49 · show in text ↑
e2

“germinated ‘DT51’ had higher GABA and lower genistein and daidzein content than germinated ‘DT84’”

✓ located verbatim in the source · abstract, characters 941–1038 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

HPLC-UV-VIS methods for GABA, daidzein, genistein and glycitein in germinated soybean were optimized

Taxon
germinated soybeanse4
Outcome
methods for determination of GABA, daidzein, genistein, and glyciteine4

Not reportedComparator

Verbatim

e4

“methods for determination of GABA, daidzein, genistein, and glycitein in germinated soybeans using high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV-VIS) were optimized”

✓ located verbatim in the source · abstract, characters 270–470 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Germination is a low-cost processing that the authors consider able to boost the nutritional quality of soybean

Taxon
soybeanGlycine maxe5
Treatment
germinatione5
Outcome
nutritional qualitye5
Direction
booste5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e5

“showed potential of germination as a low-cost processing to boost the nutritional quality of soybean”

✓ located verbatim in the source · abstract, characters 1255–1355 · show in text ↑

processing · quality: grain · Glycine max (soybean)Positive and negative results

10 papers: 6 positive, 4 negative or no effect

5 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. no effect
  3. no effect

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.