Abstract
L.) leaves under different light conditions (dark, light, irradiation by red and far-red light). Incubation in the light and irradiation by red light exhibited an activating effect on DNA adenine methylase activity, which was reflected in an increase in the number of methylated adenines in GATC sites. Far-red light and darkness exhibited an opposite effect. The use of nitrite conversion of DNA followed by methyladenine-dependent restriction by MboI nuclease revealed a phytochrome B-dependent mechanism of regulation of the methyl status of adenine in the GATC sites in the promoter of the gene encoding the mitochondrial isoform of citrate synthase. Irradiation of plants with red light caused changes in the adenine methyl status of the analyzed amplicon, as evidenced by the presence of restriction products of 290, 254, and 121 nucleotides. Adenine methylation occurred at all three GATC sites in the analyzed DNA sequence. It is concluded that adenylate methylation is controlled by phytochrome B via the transcription factor PIF4 and represents an important mechanism for the tricarboxylic acid cycle regulation by light.
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Claims 8
Claim · model-written summary
Light incubation increased DNA adenine methylase activity and GATC adenine methylation.
- Treatment
- Incubation in the lighte0
- Gene
- DNA adenine methylasee0
- Outcome
- DNA adenine methylase activitye0
- Direction
- activating effecte0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incubation in the light and irradiation by red light exhibited an activating effect on DNA adenine methylase activity, which was reflected in an increase in the number of methylated adenines in GATC sites.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
Red light increased DNA adenine methylase activity and GATC adenine methylation.
- Treatment
- irradiation by red lighte0
- Gene
- DNA adenine methylasee0
- Outcome
- DNA adenine methylase activitye0
- Direction
- activating effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incubation in the light and irradiation by red light exhibited an activating effect on DNA adenine methylase activity, which was reflected in an increase in the number of methylated adenines in GATC sites.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
Far-red light had an opposite effect on adenine methylase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Far-red light and darkness exhibited an opposite effect.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
Darkness had an opposite effect on adenine methylase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Far-red light and darkness exhibited an opposite effect.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
The assay revealed phytochrome B-dependent regulation of methylation at GATC sites in the citrate synthase promoter.
- Gene
- phytochrome Be0
- Outcome
- methyl status of adenine in the GATC sitese0
- Qualifier
- promoter of the gene encoding the mitochondrial isoform of citrate synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed a phytochrome B-dependent mechanism of regulation of the methyl status of adenine in the GATC sites in the promoter of the gene encoding the mitochondrial isoform of citrate synthase.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
Red light changed adenine methylation status in the analyzed amplicon.
- Treatment
- red lighte0
- Outcome
- adenine methyl status of the analyzed amplicone0
- Direction
- changese0
- Qualifier
- restriction products of 290, 254, and 121 nucleotidese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irradiation of plants with red light caused changes in the adenine methyl status of the analyzed amplicon, as evidenced by the presence of restriction products of 290, 254, and 121 nucleotides.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
All three GATC sites in the analyzed sequence were adenine-methylated.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adenine methylation occurred at all three GATC sites in the analyzed DNA sequence.”
t0“Maize (Zea mays L.)”
Claim · model-written summary
The authors concluded that phytochrome B controls adenylate methylation through PIF4, contributing to light regulation of the tricarboxylic acid cycle.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adenylate methylation is controlled by phytochrome B via the transcription factor PIF4 and represents an important mechanism for the tricarboxylic acid cycle regulation by light.”
t0“Maize (Zea mays L.)”
The same questions in other papers
radiation · physiology: enzyme activity · Zea mays (maize)Positive and negative results
2 in the opposite direction
- negative
- negative
light · physiology: enzyme activity · Zea mays (maize)Positive and negative results
11 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.