Abstract
This study aimed to investigate the sugar beet genotypes for resistance to hyperthermia and obtain temperatureresistant lines. Nine hybrids and one variety of sugar beet were screened. Cotyledonary leaves and hypocotyls isolated from aseptic seedlings were used for induction of callus and subsequent subcultivation. To create hyperthermic conditions, the callus lines were maintained in thermostats at temperatures of +27 C, +41 C, +45 C, and +47 C. The effect of high temperatures on the callus tissue was assessed by the specific callus diameter index. The free proline was detected with chromatography. As result of callus tissue exposed to temperatures of +41 C, 45 C and 47 C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues. At a moderate temperature (+41 C), the growth of the callus mass was somewhat higher compared to the control. At a high temperature (+45 C), the intensity of the growth processes decreased and ceased at a temperature of +47 C. After transfer and subsequent cultivation of callus tissues in regeneration medium MSR -Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants. The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes. Consequently, the temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Callus size and coloration differed significantly at high temperatures on day nine.
- Taxon
- Sugar Beet
Beta vulgaris subsp. vulgarist0 - Treatment
- temperatures of +41 C, 45 C and 47 Ce0
- Outcome
- size and colouration of the callus tissuese0
- Qualifier
- 9th day of cultivatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus tissue exposed to temperatures of +41 C, 45 C and 47 C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues”
t0“Sugar Beet”
Claim · model-written summary
Callus mass growth was somewhat higher at +41 C than control.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- moderate temperature (+41 C)e0
- Outcome
- growth of the callus masse0
- Direction
- highere0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At a moderate temperature (+41 C), the growth of the callus mass was somewhat higher compared to the control.”
t0“Sugar Beet”
Claim · model-written summary
Callus growth intensity decreased at +45 C.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- high temperature (+45 C)e0
- Outcome
- intensity of the growth processese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At a high temperature (+45 C), the intensity of the growth processes decreased”
t0“Sugar Beet”
Claim · model-written summary
Callus growth ceased at +47 C.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- temperature of +47 Ce0
- Outcome
- intensity of the growth processese1
- Direction
- ceasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ceased at a temperature of +47 C”
e1“the intensity of the growth processes decreased and ceased at a temperature of +47 C”
t0“Sugar Beet”
Claim · model-written summary
All genotypes formed morphological structures initiating regenerated plants after transfer to regeneration medium.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- regeneration medium MSR -Murashige and Skoog medium for regeneratione0
- Outcome
- formation of morphological structures that initiated the formation of regenerated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After transfer and subsequent cultivation of callus tissues in regeneration medium MSR -Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants.”
t0“Sugar Beet”
Claim · model-written summary
Regenerated plant number varied markedly across temperatures.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Treatment
- temperaturese0
- Outcome
- number of regenerated plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of regenerated plants largely fluctuated over temperatures”
t0“Sugar Beet”
Claim · model-written summary
Regenerated plant number was almost unrelated to genotype.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Material
- genotypese0
- Outcome
- number of regenerated plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes.”
t0“Sugar Beet”
Claim · model-written summary
The heat-selected lines differed in specific callus diameter.
- Taxon
- Sugar BeetBeta vulgaris subsp. vulgarist0
- Material
- temperature-resistant linese0
- Treatment
- extreme heat treatmente0
- Outcome
- specific diameter of calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus”
t0“Sugar Beet”
The same questions in other papers
heat · growth · Beta vulgaris (beet)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.