Abstract
As an important microbial resource, Actinomycetes, especially Streptomyces, have important application values in medicine and biotechnology. Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato. To obtain pure active compounds, SYH3 fermentation broth was subjected to XAD-16 macroporous resin and silica gel column chromatography. Combined with the repeated preparation and separation of preparative high-performance liquid chromatography (HPLC), a total of four monomer compounds were obtained after activity tracking. Compound 4 was identified as a new six-membered lactone ring compound named 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one by 1D and 2D nuclear magnetic resonance (NMR) data and mass spectrometry (MS). The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively. Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL. Compound 4 also had broad-spectrum antifungal activity and had a good antifungal effect on the tested plant-pathogenic fungi. The modes of action of new compound (4) still require further investigation, representing a novel and effective anti-fungal agent for future application.
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Claims 10
Claim · model-written summary
Streptomyces fungicidicus SYH3 showed good inhibitory effects on Alternaria solani in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Streptomyces fungicidicus SYH3e0
- Interactor
- Alternaria solani
Alternaria solanie0pathogen - Second interactor
- Streptomyces fungicidicus SYH3Streptomyces fungicidicuse0biocontrol_agent
- Outcome
- inhibitory effects on Alternaria solanie0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Fermentation fractionation of SYH3 yielded a total of four monomer compounds.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- monomer compoundse1
- Value
- a total of foure1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a total of four monomer compounds were obtained after activity tracking”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Compound 4 was identified as a new six-membered lactone ring compound.
- Taxon
- tomatoSolanum lycopersicume0
- Compound
- 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-onee2
- Outcome
- identified as a new six-membered lactone ring compounde2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Compound 4 was identified as a new six-membered lactone ring compound named 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
One cyclodipeptide had an IC50 of 43.4 against Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- A. solaniAlternaria solanie3pathogen
- Compound
- cyclodipeptidee3
- Outcome
- half maximal inhibitory concentration (IC50) values against A. solanie3
- Value
- 43.4e3
- Qualifier
- μg/mLe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
A second cyclodipeptide had an IC50 of 42.9 against Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- A. solaniAlternaria solanie3pathogen
- Compound
- cyclodipeptidee3
- Outcome
- half maximal inhibitory concentration (IC50) values against A. solanie3
- Value
- 42.9e3
- Qualifier
- μg/mLe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
A third cyclodipeptide had an IC50 of 30.6 μg/mL against Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- A. solaniAlternaria solanie3pathogen
- Compound
- cyclodipeptidee3
- Outcome
- half maximal inhibitory concentration (IC50) values against A. solanie3
- Value
- 30.6 μg/mLe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Compound 4 significantly inhibited spore germination of Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Compound 4e4
- Interactor
- A. solaniAlternaria solanie4pathogen
- Outcome
- spore germinatione4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Compound 4 induced swollen and deformed local hyphae of Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Compound 4e4
- Interactor
- A. solaniAlternaria solanie4pathogen
- Outcome
- swollen and deformed local hyphaee4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Compound 4 had an IC50 of 24.9 μg/mL against Alternaria solani.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- A. solaniAlternaria solanie4pathogen
- Compound
- Compound 4e4
- Outcome
- IC50 valuee4
- Value
- 24.9 μg/mLe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
Claim · model-written summary
Compound 4 has broad-spectrum antifungal activity against tested plant-pathogenic fungi.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- plant-pathogenic fungie5pathogen
- Compound
- Compound 4e5
- Outcome
- broad-spectrum antifungal activitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Compound 4 also had broad-spectrum antifungal activity and had a good antifungal effect on the tested plant-pathogenic fungi”
e0“Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.