Abstract
Vanda bicolor Griff. is a vulnerable orchid species having immense horticultural importance. This communication reports the outcome of a successful attempt towards the formulation of immature seed culture protocol of Vanda bicolor, factors affecting effective germination, plant regeneration. This study also endeavored to formulation of a simple and effective hardening process which supports direct transfer of plantlets from the culture condition to the field. Immature seeds from green pods of 7 months old registered 88.2% germination on MS nutrient medium supplemented with 3% sucrose, 3 μM each of NAA and BA under normal culture conditions. The germinating embryos converted to protocorms and protocorm-like bodies (PLBs) on the same germination medium within 55 days of culture and subsequently converted to plantlets and supported culture proliferation and simultaneous healthy roots development on MS medium fortified with sucrose (3%), NAA and BA (3 + 6 μM respectively) and activated charcoal (0.6%) where as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant. A new hardening technique was developed with fortified pieces of dead wood and burnt wood. It was found that after 5–6 wk of transfer to the hardening condition, velamenous roots started adhering to the wood pieces. These plants could be transferred to polyhouse as well as in the nature directly where 98.25 and 97.75% survival rate registered respectively after 8 wk of transfer.
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Claims 8
Claim · model-written summary
Seven-month immature seeds germinated at 88.2% on supplemented MS medium.
- Taxon
- Vanda bicolor Griff.
Vanda bicolort0 - Organ / stage
- Immature seedse0
- Treatment
- MS nutrient medium supplemented with 3% sucrose, 3 μM each of NAA and BAe0
- Outcome
- germinatione0
- Value
- 88.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immature seeds from green pods of 7 months old registered 88.2% germination on MS nutrient medium supplemented with 3% sucrose, 3 μM each of NAA and BA under normal culture conditions”
t0“Vanda bicolor Griff.”
Claim · model-written summary
Germinating embryos became protocorms and protocorm-like bodies within 55 days.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Organ / stage
- germinating embryose0
- Outcome
- converted to protocorms and protocorm-like bodies (PLBs)e0
- Value
- 55 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The germinating embryos converted to protocorms and protocorm-like bodies (PLBs) on the same germination medium within 55 days of culture”
t0“Vanda bicolor Griff.”
Claim · model-written summary
The supplemented medium supported conversion to plantlets and root development.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Treatment
- MS medium fortified with sucrose (3%), NAA and BA (3 + 6 μM respectively) and activated charcoal (0.6%)e0
- Outcome
- converted to plantlets and supported culture proliferation and simultaneous healthy roots developmente0
- Value
- 6.2 shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subsequently converted to plantlets and supported culture proliferation and simultaneous healthy roots development on MS medium fortified with sucrose (3%), NAA and BA (3 + 6 μM respectively) and activated charcoal (0.6%) where as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
t0“Vanda bicolor Griff.”
Claim · model-written summary
The medium produced 6.2 shoots per subculture per PLB.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Outcome
- shoots developed per subculture per PLBse0
- Value
- 6.2 shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
t0“Vanda bicolor Griff.”
Claim · model-written summary
The medium produced 3.3 roots per plant.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Outcome
- roots per plante0
- Value
- 3.3 rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as many as 6.2 shoots developed per subculture per PLBs and 3.3 roots per plant”
t0“Vanda bicolor Griff.”
Claim · model-written summary
Hardened roots adhered to wood pieces after five to six weeks.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Outcome
- velamenous roots started adhering to the wood piecese0
- Value
- 5–6 wke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after 5–6 wk of transfer to the hardening condition, velamenous roots started adhering to the wood pieces”
t0“Vanda bicolor Griff.”
Claim · model-written summary
After direct polyhouse transfer, survival was 98.25% at eight weeks.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Outcome
- survival ratee0
- Value
- 98.25e0
- Setting
- polyhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants could be transferred to polyhouse as well as in the nature directly where 98.25 and 97.75% survival rate registered respectively after 8 wk of transfer”
t0“Vanda bicolor Griff.”
Claim · model-written summary
After direct transfer to nature, survival was 97.75% at eight weeks.
- Taxon
- Vanda bicolor Griff.Vanda bicolort0
- Outcome
- survival ratee0
- Value
- 97.75%e0
- Setting
- in the naturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants could be transferred to polyhouse as well as in the nature directly where 98.25 and 97.75% survival rate registered respectively after 8 wk of transfer”
t0“Vanda bicolor Griff.”