Abstract
BACKGROUND: Water deficit (WD) has serious effect on the productivity of crops. Formation of cuticular layer with increased content of wax and cutin on leaf surfaces is closely related to drought tolerance. Identification of drought tolerance associated wax components and cutin monomers and the genes responsible for their biosynthesis is essential for understanding the physiological and genetic mechanisms underlying drought tolerance and improving crop drought resistance. RESULT: In this study, we conducted comparative phenotypic and transcriptomic analyses of two Gossypium hirsutum varieties that are tolerant (XL22) or sensitive (XL17) to drought stress. XL17 consumed more water than XL22, particularly under the WD conditions. WD significantly induced accumulation of most major wax components (C29 and C31 alkanes) and cutin monomers (palmitic acid and stearic acid) in leaves of both XL22 and XL17, although accumulation of the major cutin monomers, i.e., polyunsaturated linolenic acid (C18:3n-3) and linoleic acid (C18:2n-6), were significantly repressed by WD in both XL22 and XL17. According to the results of transcriptome analysis, although many genes and their related pathways were commonly induced or repressed by WD in both XL22 and XL17, WD-induced differentially expressed genes specific to XL22 or XL17 were also evident. Among the genes that were commonly induced by WD were the GhCER1 genes involved in biosynthesis of alkanes, consistent with the observation of enhanced accumulation of alkanes in cotton leaves under the WD conditions. Interestingly, under the WD conditions, several GhCYP86 genes, which encode enzymes catalyzing the omega-hydroxylation of fatty acids and were identified to be the hub genes of one of the co-expression gene modules, showed a different expression pattern between XL22 and XL17 that was in agreement with the WD-induced changes of the content of hydroxyacids or fatty alcohols in these two varieties. CONCLUSION: The results contribute to our comprehending the physiological and genetic mechanisms underlying drought tolerance and provide possible solutions for the difference of drought resistance of different cotton varieties.
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Claims 8
Claim · model-written summary
Drought-sensitive XL17 consumed more water than tolerant XL22, especially under water deficit.
- Taxon
- cotton
Gossypium hirsutumt0 - Material
- XL17e0
- Treatment
- WDe0
- Outcome
- consumed more watere0
- Direction
- moree0
- Comparator
- XL22e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XL17 consumed more water than XL22, particularly under the WD conditions.”
t0“cotton”
Claim · model-written summary
Water deficit increased leaf C29 and C31 alkanes in both cotton varieties.
- Taxon
- cottonGossypium hirsutumt0
- Material
- Gossypium hirsutum L.t0
- Organ / stage
- leaft0
- Treatment
- WDe0
- Outcome
- C29 and C31 alkanese0
- Direction
- induced accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WD significantly induced accumulation of most major wax components (C29 and C31 alkanes)”
t0“leaf cuticle lipids and gene expression networks in cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Water deficit increased leaf palmitic and stearic acid cutin monomers in both varieties.
- Taxon
- cottonGossypium hirsutumt0
- Material
- XL22 and XL17e0
- Organ / stage
- leavese0
- Treatment
- water deficiencyt0
- Outcome
- palmitic acid and stearic acide0
- Direction
- induced accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WD significantly induced accumulation of most major wax components (C29 and C31 alkanes) and cutin monomers (palmitic acid and stearic acid) in leaves of both XL22 and XL17”
t0“water deficiency on leaf cuticle lipids and gene expression networks in cotton”
Claim · model-written summary
Water deficit repressed leaf linolenic and linoleic acid accumulation in both varieties.
- Taxon
- cottonGossypium hirsutumt0
- Material
- XL22 and XL17e0
- Treatment
- WDe0
- Outcome
- polyunsaturated linolenic acid (C18:3n-3) and linoleic acid (C18:2n-6)e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polyunsaturated linolenic acid (C18:3n-3) and linoleic acid (C18:2n-6), were significantly repressed by WD in both XL22 and XL17”
t0“cotton”
Claim · model-written summary
Water deficit induced or repressed many shared genes and pathways in XL22 and XL17.
- Taxon
- cottonGossypium hirsutumt0
- Material
- XL22 and XL17e0
- Treatment
- WDe0
- Outcome
- genes and their related pathwayse0
- Direction
- induced or repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many genes and their related pathways were commonly induced or repressed by WD in both XL22 and XL17”
t0“cotton”
Claim · model-written summary
Water deficit also produced variety-specific differentially expressed genes in XL22 or XL17.
- Taxon
- cottonGossypium hirsutumt0
- Material
- XL22 or XL17e0
- Treatment
- WDe0
- Outcome
- differentially expressed genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WD-induced differentially expressed genes specific to XL22 or XL17 were also evident”
t0“cotton”
Claim · model-written summary
Water deficit commonly induced alkane biosynthesis GhCER1 genes.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes that were commonly induced by WD were the GhCER1 genes involved in biosynthesis of alkanes”
t0“cotton”
Claim · model-written summary
Under water deficit, several GhCYP86 genes differed in expression between XL22 and XL17 in agreement with hydroxyacid or fatty alcohol changes.
- Taxon
- cottonGossypium hirsutumt0
- Material
- XL22 and XL17e0
- Treatment
- WDe0
- Gene
- GhCYP86e0
- Outcome
- different expression patterne0
- Qualifier
- WD-induced changes of the content of hydroxyacids or fatty alcoholse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several GhCYP86 genes, which encode enzymes catalyzing the omega-hydroxylation of fatty acids and were identified to be the hub genes of one of the co-expression gene modules, showed a different expression pattern between XL22 and XL17 that was in agreement with the WD-induced changes of the content of hydroxyacids or fatty alcohols”
t0“cotton”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.