Abstract
The effects of medium strength as well as different antioxidant types and concentrations on adventitious shoot bud multiplication, hyperhydricity and tissue browning of date palm cv. Aziza Bouzid were evaluated. Organogenic cultures of date palm cv. Aziza Bouzid were cultured on semi-solid Murashige and Skoog medium at half-strength (MS/2) or one-third-strength (MS/3), supplemented with 0.5 mg/L 2-naphthoxyacetic acid (NOA), 0.5 mg/L kinetin, and different concentrations of activated charcoal and polyvinylpyrrolidone (PVP). The findings of the present study showed that the use MS/3 media resulted in lower hyperhydricity rates than MS/2 media. Besides, activated charcoal significantly reduced tissue browning. The lowest rates of hyperhydricity (35%) and tissue browning (20%) were observed in MS/3 medium supplemented with 1.5 g/L activated charcoal. Interestingly, this culture medium showed also the highest number of adventitious shoot buds per explant (35.20), with a significant difference with all the other media (25.55-30.15 adventitious shoot buds per explant). Increasing the concentration of activated charcoal to 2 g/L did not decreased the browning rate. The use of PVP alone showed tissue browning rates varying from 30 to 55% while combining PVP and activated charcoal showed browning percentages ranging from 20 to 30%. Shoot elongation and rooting were successfully achieved and plantlet were acclimatized and showed normal growth and development under the greenhouse conditions.
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Claims 10
Claim · model-written summary
MS/3 medium resulted in lower hyperhydricity rates than MS/2 medium in date palm cv. Aziza Bouzid cultures.
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- Aziza Bouzidt0
- Treatment
- MS/3 mediae0
- Outcome
- hyperhydricity ratese0
- Direction
- lowere0
- Comparator
- MS/2 mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the use MS/3 media resulted in lower hyperhydricity rates than MS/2 media”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Activated charcoal significantly reduced tissue browning.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- activated charcoale0
- Outcome
- tissue browninge0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activated charcoal significantly reduced tissue browning”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
The lowest hyperhydricity rate (35%) was observed in MS/3 medium supplemented with 1.5 g/L activated charcoal.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- MS/3 medium supplemented with 1.5 g/L activated charcoale0
- Outcome
- rates of hyperhydricitye0
- Direction
- loweste0
- Value
- 35%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowest rates of hyperhydricity (35%) and tissue browning (20%) were observed in MS/3 medium supplemented with 1.5 g/L activated charcoal”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
The lowest tissue browning rate (20%) was observed in MS/3 medium supplemented with 1.5 g/L activated charcoal.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- MS/3 medium supplemented with 1.5 g/L activated charcoale0
- Outcome
- tissue browninge0
- Direction
- loweste0
- Value
- 20%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowest rates of hyperhydricity (35%) and tissue browning (20%) were observed in MS/3 medium supplemented with 1.5 g/L activated charcoal”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
MS/3 medium with 1.5 g/L activated charcoal gave the highest number of adventitious shoot buds per explant (35.20), significantly different from the other media (25.55-30.15).
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- this culture mediume0
- Outcome
- adventitious shoot buds per explante0
- Direction
- higheste0
- Value
- 35.20e0
- Qualifier
- 25.55-30.15 adventitious shoot buds per explante0
- Comparator
- all the other mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this culture medium showed also the highest number of adventitious shoot buds per explant (35.20), with a significant difference with all the other media (25.55-30.15 adventitious shoot buds per explant)”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Increasing the activated charcoal concentration to 2 g/L did not decrease the browning rate.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- Increasing the concentration of activated charcoal to 2 g/Le0
- Outcome
- browning ratee0
- Direction
- did not decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the concentration of activated charcoal to 2 g/L did not decreased the browning rate”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
PVP alone gave tissue browning rates of 30 to 55%.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- PVP alonee0
- Outcome
- tissue browning ratese0
- Value
- 30 to 55%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of PVP alone showed tissue browning rates varying from 30 to 55%”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Combining PVP and activated charcoal gave tissue browning of 20 to 30%.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- combining PVP and activated charcoale0
- Outcome
- browning percentagese0
- Value
- 20 to 30%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“combining PVP and activated charcoal showed browning percentages ranging from 20 to 30%”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Shoot elongation and rooting were successfully achieved.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Outcome
- Shoot elongation and rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot elongation and rooting were successfully achieved”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Plantlets were acclimatized and showed normal growth and development under greenhouse conditions.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Outcome
- normal growth and developmente0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantlet were acclimatized and showed normal growth and development under the greenhouse conditions”
t0“date palm cv. Aziza Bouzid”
The same questions in other papers
charcoal · disease · Phoenix dactylifera (date palm)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.