Abstract
The K homology (KH) repeat is an RNA-binding motif that exists in various proteins, some of which participate in plant growth. However, the function of KH domain-containing proteins in plant defence is still unclear. In this study, we found that a KH domain-containing protein in apple (Malus domestica), HEN4-like (MdKRBP4), is involved in the plant immune response. Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali, whereas transient overexpression of MdKRBP4 stimulated ROS accumulation in apple leaves, indicating that MdKRBP4 is a positive immune regulator. Additionally, MdKRBP4 was proven to interact with the VmEP1 effector secreted by V. mali, which led to decreased accumulation of MdKRBP4. Coexpression of MdKRBP4 with VmEP1 inhibited cell death and ROS production induced by MdKRBP4 in Nicotiana benthamiana. These results indicate that MdKRBP4 functions as a novel positive regulatory factor in plant immunity in M. domestica and is a virulence target of the V. mali effector VmEP1.
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Claims 8
Claim · model-written summary
Silencing of MdKRBP4 compromised ROS production in apple.
- Taxon
- apple
Malus domesticae0 - Treatment
- Silencing of MdKRBP4e0
- Gene
- MdKRBP4e0
- Outcome
- reactive oxygen species (ROS) productione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali”
Claim · model-written summary
Silencing of MdKRBP4 enhanced the susceptibility of apple to Valsa mali.
- Taxon
- appleMalus domesticae0
- Treatment
- Silencing of MdKRBP4e0
- Interactor
- Valsa maliCytospora malie0pathogen
- Gene
- MdKRBP4e0
- Outcome
- susceptibility of apple to Valsa malie0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali”
Claim · model-written summary
Transient overexpression of MdKRBP4 stimulated ROS accumulation in apple leaves.
- Taxon
- appleMalus domesticae1
- Organ / stage
- apple leavese1
- Treatment
- transient overexpression of MdKRBP4e1
- Gene
- MdKRBP4e1
- Outcome
- ROS accumulatione1
- Direction
- stimulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“transient overexpression of MdKRBP4 stimulated ROS accumulation in apple leaves”
Claim · model-written summary
MdKRBP4 is a positive immune regulator in apple, involved in the plant immune response.
- Taxon
- appleMalus domesticae0
- Gene
- MdKRBP4e2
- Outcome
- plant immune responsee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali”
e2“indicating that MdKRBP4 is a positive immune regulator”
e3“is involved in the plant immune response”
Claim · model-written summary
MdKRBP4 interacts with the VmEP1 effector secreted by V. mali.
- Taxon
- appleMalus domesticae0
- Interactor
- V. maliCytospora malie4pathogen
- Gene
- MdKRBP4e4
- Outcome
- interact with the VmEP1 effectore4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali”
e4“MdKRBP4 was proven to interact with the VmEP1 effector secreted by V. mali, which led to decreased accumulation of MdKRBP4”
Claim · model-written summary
Interaction with VmEP1 is associated with decreased accumulation of MdKRBP4.
- Taxon
- appleMalus domesticae0
- Interactor
- V. maliCytospora malie4pathogen
- Gene
- MdKRBP4e4
- Outcome
- accumulation of MdKRBP4e4
- Direction
- decreasede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of MdKRBP4 compromised reactive oxygen species (ROS) production and enhanced the susceptibility of apple to Valsa mali”
e4“MdKRBP4 was proven to interact with the VmEP1 effector secreted by V. mali, which led to decreased accumulation of MdKRBP4”
Claim · model-written summary
Coexpression of MdKRBP4 with VmEP1 inhibited cell death and ROS production induced by MdKRBP4 in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae5 - Treatment
- Coexpression of MdKRBP4 with VmEP1e5
- Gene
- MdKRBP4e5
- Outcome
- cell death and ROS production induced by MdKRBP4e5
- Direction
- inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Coexpression of MdKRBP4 with VmEP1 inhibited cell death and ROS production induced by MdKRBP4 in Nicotiana benthamiana”
Claim · model-written summary
MdKRBP4 is a virulence target of the V. mali effector VmEP1.
- Taxon
- M. domesticaMalus domesticae6
- Interactor
- V. maliCytospora malie6pathogen
- Gene
- MdKRBP4e6
- Outcome
- virulence targete6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“MdKRBP4 functions as a novel positive regulatory factor in plant immunity in M. domestica and is a virulence target of the V. mali effector VmEP1”
The same questions in other papers
loss of function · physiology: oxidative · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
loss of function · Cytospora mali · Malus domestica (apple)Positive and negative results
8 papers in the same direction as this paper · 10 in the opposite direction
- positive
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.