Abstract
To isolate the genes involved in the response of graminaceous plants to Fe‐deficient stress, a protein induced by Fe‐deficiency treatment was isolated from barley (Hordeum vulgare L.) roots. Based on the partial amino acid sequence of this protein, a cDNA (HvAPT1) encoding adenine phosphoribosyltransferase (APRT: EC 2.4.2.7) was cloned from a cDNA library prepared from Fe‐deficient barley roots. Southern analysis suggested that there were at least two genes encoding APRT in barley. Fe deficiency increased HvAPT1 expression in barley roots and resupplying Fe to the Fe‐deficient plants rapidly negated the increase in HvAPT1 mRNA. Analysis of localization of HvAPT1‐ sGFP fusion proteins in tobacco BY‐2 cells indicated that the protein from HvAPT1 was localized in the cytoplasm of cells. Consistent with the results of Northern analysis, the enzymatic activity of APRT in barley roots was remarkably increased by Fe deficiency. This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice. In contrast, the induction was not observed to occur in the roots of a non‐graminaceous plant, tobacco. Graminaceous plants generally synthesize the mugineic acid family phytosiderophores (MAs) in roots under Fe‐deficient conditions. In this paper, a possible role of HvAPT1 in the biosynthesis of MAs related to adenine salvage in the methionine cycle is discussed.
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Claims 10
Claim · model-written summary
HvAPT1 encoding APRT was cloned from iron deficient barley roots.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- rootse0
- Gene
- HvAPT1e0
- Outcome
- a cDNA (HvAPT1) encoding adenine phosphoribosyltransferasee0
- Value
- a cDNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a cDNA (HvAPT1) encoding adenine phosphoribosyltransferase (APRT: EC 2.4.2.7) was cloned from a cDNA library prepared from Fe‐deficient barley roots”
Claim · model-written summary
At least two genes encode APRT in barley.
- Taxon
- barleyHordeum vulgaree0
- Gene
- APRTe0
- Outcome
- at least two genes encoding APRTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Southern analysis suggested that there were at least two genes encoding APRT in barley”
Claim · model-written summary
Iron deficiency increased HvAPT1 expression in barley roots.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- rootse0
- Treatment
- Fe deficiencye0
- Gene
- HvAPT1e0
- Outcome
- HvAPT1 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fe deficiency increased HvAPT1 expression in barley roots”
Claim · model-written summary
Resupplying iron rapidly negated the increase in HvAPT1 mRNA in deficient plants.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- resupplying Fee0
- Gene
- HvAPT1e0
- Outcome
- HvAPT1 mRNAe0
- Direction
- negated the increasee0
- Comparator
- Fe‐deficient plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resupplying Fe to the Fe‐deficient plants rapidly negated the increase in HvAPT1 mRNA”
t0“barley roots”
Claim · model-written summary
The HvAPT1 protein localized in the cytoplasm of tobacco BY 2 cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- BY‐2e0
- Gene
- HvAPT1e0
- Outcome
- localized in the cytoplasm of cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HvAPT1‐ sGFP fusion proteins in tobacco BY‐2 cells indicated that the protein from HvAPT1 was localized in the cytoplasm of cells”
Claim · model-written summary
Iron deficiency markedly increased APRT enzymatic activity in barley roots.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- rootse0
- Treatment
- Fe deficiencye0
- Gene
- APRTe0
- Outcome
- enzymatic activity of APRTe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enzymatic activity of APRT in barley roots was remarkably increased by Fe deficiency”
Claim · model-written summary
Iron deficiency induced APRT activity in rye roots.
- Taxon
- rye
Secale cerealee0 - Organ / stage
- rootse0
- Treatment
- Fe deficiencye0
- Gene
- APRTe0
- Outcome
- APRT activitye0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice”
Claim · model-written summary
Iron deficiency induced APRT activity in maize roots.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice”
Claim · model-written summary
Iron deficiency induced APRT activity in rice roots.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- rootse0
- Treatment
- Fe deficiencye0
- Gene
- APRTe0
- Outcome
- APRT activitye0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice”
Claim · model-written summary
Iron deficiency did not induce APRT activity in tobacco roots.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- rootse0
- Treatment
- Fe deficiencye1
- Gene
- APRTe1
- Outcome
- APRT activitye1
- Direction
- not observede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the induction was not observed to occur in the roots of a non‐graminaceous plant, tobacco”
e1“This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice”
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This paper is about lacking the nutrient, so it counts the other way round here.
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.