Abstract
PDF HTML阅读 XML下载 导出引用 引用提醒 水稻根系响应镉胁迫的蛋白质差异表达 DOI: 10.5846/stxb201403230517 作者: 作者单位: 福建农林大学生命科学学院,福建农林大学,福建农林大学,福建农林大学生命科学学院,福建农林大学,福建农林大学,福建农林大学生命科学学院,福建农林大学,淮南师范学院生命科学系,福建农林大学生命科学学院,福建农林大学 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目(31070403);福建省自然科学基金项目(2009J01056,2013J01083);福建省教育厅基金项目(JA09084);福建省高校服务海西建设重点项目(0B08B005);福建农林大学重点项目建设专项(6112c0604) Analysis of the differently expressed proteins in rice roots in response to cadmium stress Author: Affiliation: School of life sciences, Fujian Agriculture and Forestry University,,,,,,School of life sciences, Fujian Agriculture and Forestry University,,,, Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为探讨水稻根系对镉胁迫的分子生理响应,以抗镉水稻PI312777和镉敏感水稻IR24为材料,设置Cd2+浓度为0、50和100 μmol/L的水培试验,处理7 d后分析了水稻根系的蛋白质差异表达。结果表明,在镉胁迫下水稻PI312777和IR24根系有18个蛋白质发生了差异表达,其中的12个得到MALDI-TOF/MS鉴定。这些鉴定的蛋白功能可分四类:(1)与活性氧(ROS)胁迫相关的过氧化物酶(POD)、蛋氨酸腺苷转移酶(MAT)、类萌发素蛋白前体;(2)与谷胱甘肽(GSH)合成相关的S-腺苷甲硫氨酸合成酶(SAMS)、谷氨酰胺合成酶(GS)和谷氨酸脱氢酶(GDH);(3)与逆境胁迫相关的ABA胁迫诱导蛋白含HVA22域蛋白、ABA-胁迫-成熟诱导蛋白5(ASR5);(4)与细胞分裂调控相关的GTP结合核蛋白Ran-2。镉胁迫下SAMS和GTP结合核蛋白Ran-2在两种水稻根系均发生上调表达;MAT、POD、类萌发素蛋白前体和GS发生下调表达;依赖NADP-GDH、GDH和磷酸甘油酸变位酶在IR24根部均发生下调表达,在PI312777根部仅在100 μmol/L Cd2+处理发生下调表达;含HVA22域蛋白在PI312777根部上调表达,在IR24根部发生下调表达;ASR5在PI312777根部上调表达,在IR24根部的表达无显著差异;100 μmol/L Cd2+胁迫下60S酸性核糖体蛋白P0在水稻PI312777根部表达下调,在IR24根部表达上调。可见,镉胁迫使水稻根部ROS增加,形成氧化胁迫反应,造成毒害作用,而水稻根通过调节SAMS和GS提高GSH合成降低镉毒害。ASR5和HVA22蛋白等逆境胁迫蛋白的表达差异则是水稻品种间抗性差异的重要原因之一。 Abstract:To investigate the molecular and physiological responses in rice roots to cadmium (Cd) stress, a set of hydroponic culture experiments treated with Cd2+ at 0, 50, and 100 μmol/L in the solutions were conducted to analyze the differentially expressed proteins in the roots of two rice (Oryza sativa L.) cultivars (Cd-tolerant rice PI312777 and Cd-sensitive rice IR24) 7 days after the treatments. The results showed that 18 proteins were differentially expressed in the roots of PI312777 and IR24 under Cd stress; 12 of them were identified by the MALDI-TOF/MS technique. The biological functions of these identified proteins were involved in four metabolic pathways, including (1) reactive oxygen species (ROS) stress: peroxidase (POD), methionine adenosyltransferase (MAT), and germin-like protein precursor; (2) glutathione (GSH) synthesis: S-adenosylmethionine synthetase (SAMS), glutamine synthetase (GS), and glutamate dehydrogenase (GDH); (3) stress response induced by abscisic acid (ABA): HVA22 and abscisic acid-stress-ripening-inducible 5 protein (ASR5); and (4) cell division regulation: GTP-binding nuclear protein Ran-2.Under Cd stress conditions, the expression of SAMS and GTP-binding nuclear protein Ran-2 were up-regulated and MAT, POD, the germin-like protein precursor, and GS were down-regulated in the roots of the two rice cultivars. The expression of NADP-GDH, GDH and phosphoglycerate mutase were down-regulated in the roots of rice IR24, but they were down-regulated in PI312777 only upon the treatment with 100 μmol/L Cd2+. The expression of protein with HVA22 domain was up-regulated in PI312777, but it was downregulated reversed in IR24. ASR5 was up-regulated in rice PI312777, but no significant change was found in IR24. The 60s acidic ribosomal protein P0 was down-regulated in PI312777, but up-regulated in IR24 at 100 μmol/L Cd2+condition. Our results suggest that Cd stress increases ROS and produces oxidative stress in rice roots, which lead to Cd toxicity in rice roots. To alleviate Cd toxicity, rice roots increase the GSH synthesis by up-regulation of SAMS and GS. Different expression patterns of stress-related proteins such as ASR5 and HVA22 are important in understanding the differences in Cd tolerance across rice cultivars. 参考文献 相似文献 引证文献
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Claims 16
Claim · model-written summary
Eighteen root proteins were differentially expressed under cadmium stress.
- Taxon
- rice
Oryza sativae1 - Material
- PI312777 and IR24e0
- Treatment
- Cd stresse0
- Outcome
- proteins were differentially expressede0
- Value
- 18 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“18 proteins were differentially expressed in the roots of PI312777 and IR24 under Cd stress”
e1“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
Twelve of the differentially expressed proteins were identified by MALDI-TOF/MS.
- Taxon
- riceOryza sativae1
- Outcome
- identified by the MALDI-TOF/MS techniquee0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 of them were identified by the MALDI-TOF/MS technique.”
e1“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
The identified proteins had functions in ROS stress, glutathione synthesis, ABA stress response, and cell division regulation.
- Taxon
- riceOryza sativae1
- Outcome
- four metabolic pathwayse0
- Value
- four metabolic pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The biological functions of these identified proteins were involved in four metabolic pathways, including (1) reactive oxygen species (ROS) stress: peroxidase (POD), methionine adenosyltransferase (MAT), and germin-like protein precursor; (2) glutathione (GSH) synthesis: S-adenosylmethionine synthetase (SAMS), glutamine synthetase (GS), and glutamate dehydrogenase (GDH); (3) stress response induced by abscisic acid (ABA): HVA22 and abscisic acid-stress-ripening-inducible 5 protein (ASR5); and (4) cell division regulation: GTP-binding nuclear protein Ran-2.”
e1“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
SAMS and Ran-2 expression rose under Cd stress in both rice cultivars.
- Taxon
- riceOryza sativae1
- Treatment
- Cd stresse0
- Gene
- SAMS and GTP-binding nuclear protein Ran-2e0
- Outcome
- expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under Cd stress conditions, the expression of SAMS and GTP-binding nuclear protein Ran-2 were up-regulated”
e1“in the roots of the two rice cultivars.”
Claim · model-written summary
MAT, POD, germin-like precursor, and GS expression declined under Cd stress in both rice cultivars.
- Taxon
- riceOryza sativae2
- Treatment
- Cd stresse1
- Gene
- MAT, POD, the germin-like protein precursor, and GSe0
- Outcome
- MAT, POD, the germin-like protein precursor, and GSe0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MAT, POD, the germin-like protein precursor, and GS were down-regulated in the roots of the two rice cultivars.”
e1“Under Cd stress conditions”
e2“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
NADP-GDH, GDH, and phosphoglycerate mutase expression declined in IR24 roots.
- Taxon
- riceOryza sativae0
- Material
- IR24e0
- Treatment
- Cd stresse1
- Gene
- NADP-GDH, GDH and phosphoglycerate mutasee0
- Outcome
- expressione0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of NADP-GDH, GDH and phosphoglycerate mutase were down-regulated in the roots of rice IR24”
e1“Under Cd stress conditions”
Claim · model-written summary
NADP-GDH, GDH, and phosphoglycerate mutase expression declined in PI312777 only at 100 μmol/L Cd2+.
- Taxon
- riceOryza sativae2
- Material
- PI312777e0
- Treatment
- treatment with 100 μmol/L Cd2+e0
- Gene
- NADP-GDH, GDH and phosphoglycerate mutasee1
- Outcome
- expressione1
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were down-regulated in PI312777 only upon the treatment with 100 μmol/L Cd2+.”
e1“The expression of NADP-GDH, GDH and phosphoglycerate mutase”
e2“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
HVA22 domain protein expression rose in PI312777.
- Taxon
- riceOryza sativae2
- Material
- PI312777e0
- Treatment
- Cd stresse1
- Gene
- protein with HVA22 domaine0
- Outcome
- expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of protein with HVA22 domain was up-regulated in PI312777”
e1“Under Cd stress conditions”
e2“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
HVA22 domain protein expression declined in IR24.
- Taxon
- riceOryza sativae2
- Material
- IR24e0
- Treatment
- Cd stresse1
- Gene
- protein with HVA22 domaine0
- Outcome
- expressione0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of protein with HVA22 domain was up-regulated in PI312777, but it was downregulated reversed in IR24.”
e1“Under Cd stress conditions”
e2“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
ASR5 expression rose in PI312777.
- Taxon
- riceOryza sativae0
- Material
- PI312777e0
- Treatment
- Cd stresse1
- Gene
- ASR5e0
- Outcome
- ASR5e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ASR5 was up-regulated in rice PI312777”
e1“Under Cd stress conditions”
Claim · model-written summary
ASR5 expression did not change significantly in IR24.
- Taxon
- riceOryza sativae0
- Material
- IR24e0
- Treatment
- Cd stresse1
- Gene
- ASR5e0
- Outcome
- ASR5e0
- Direction
- no significant changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ASR5 was up-regulated in rice PI312777, but no significant change was found in IR24.”
e1“Under Cd stress conditions”
Claim · model-written summary
The 60s acidic ribosomal protein P0 declined in PI312777 under 100 μmol/L Cd2+.
- Taxon
- riceOryza sativae1
- Material
- PI312777e0
- Treatment
- 100 μmol/L Cd2+e0
- Gene
- 60s acidic ribosomal protein P0e0
- Outcome
- 60s acidic ribosomal protein P0e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 60s acidic ribosomal protein P0 was down-regulated in PI312777, but up-regulated in IR24 at 100 μmol/L Cd2+condition.”
e1“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
The 60s acidic ribosomal protein P0 rose in IR24 under 100 μmol/L Cd2+.
- Taxon
- riceOryza sativae1
- Material
- IR24e0
- Treatment
- 100 μmol/L Cd2+e0
- Gene
- 60s acidic ribosomal protein P0e0
- Outcome
- 60s acidic ribosomal protein P0e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 60s acidic ribosomal protein P0 was down-regulated in PI312777, but up-regulated in IR24 at 100 μmol/L Cd2+condition.”
e1“two rice (Oryza sativa L.) cultivars”
Claim · model-written summary
Cadmium stress increased ROS and oxidative stress in rice roots, causing toxicity according to the authors.
- Taxon
- riceOryza sativae0
- Treatment
- Cd stresse0
- Outcome
- ROSe0
- Direction
- increasese0
- Qualifier
- oxidative stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd stress increases ROS and produces oxidative stress in rice roots, which lead to Cd toxicity in rice roots.”
Claim · model-written summary
The authors inferred that rice roots increase GSH synthesis by up-regulating SAMS and GS to alleviate Cd toxicity.
- Taxon
- riceOryza sativae0
- Gene
- SAMS and GSe0
- Outcome
- GSH synthesise0
- Direction
- increasee0
- Qualifier
- alleviate Cd toxicitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To alleviate Cd toxicity, rice roots increase the GSH synthesis by up-regulation of SAMS and GS.”
Claim · model-written summary
The authors associated different ASR5 and HVA22 expression patterns with cultivar differences in cadmium tolerance.
- Taxon
- riceOryza sativae0
- Gene
- ASR5 and HVA22e0
- Outcome
- differences in Cd tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different expression patterns of stress-related proteins such as ASR5 and HVA22 are important in understanding the differences in Cd tolerance across rice cultivars.”
The same questions in other papers
cadmium atom · quality: protein · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.