Paper · 2015 · Nippon Shokuhin Kagaku Kogaku Kaishi

Behavior Analysis of γ-aminobutyrate and Glutamate Decarboxylase Activity in Salted Radish Roots (Takuan-zuke)

Ryo Kato; Satomi Hayashi; Taito Kobayashi; et al.

12
claims
24
verbatim quotes
12
citations
1
research areas
✓
Open access

Abstract

We prepared salted radish roots and analyzed the changes in -aminobutyrate (GABA) and glutamate decarboxylase (GAD) activity during dehydration and long-term salting. The amounts of glutamate (Glu) and GABA in the fresh roots were 5.720.23 and 0.280.01 mg/g dry weight (DW), respectively. The amount of GABA by sundrying and salt-pressing process increased to 7.301.57 and 4.980.06 mg/g DW, respectively. In contrast, the Glu content decreased to 0.710.13 mg/g DW. The GABA content in salted radish was further increased by 1.2-3.0-fold by the addition of monosodium glutamate (MSG, 0.1-0.5 % weight of the radish) at the same time of salting. Fresh radish showed the highest GAD activity, which decreased during the dehydration and salting process. On the other hand, in the liquid leached from the radishes during the salting process (agari-mizu in Japanese) with MSG, a timedependent decrease of Glu and increase of GABA were observed. Thus, it was suggested that Glu was converted to GABA by microorganisms in the agari-mizu and transferred to the salted radish. The color of the salted radish without MSG was pale yellow, while that with MSG turned grayish in color after 2 months of salting.Thus, the addition of MSG during the salting process decreased the shelf-life of the product.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Observationobservedoriginal resultmeasured

Claim · model-written summary

Fresh radish roots contained 5.720.23 mg/g dry weight glutamate.

Organ / stage
fresh rootse0
Outcome
glutamate (Glu)e0
Value
5.720.23e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The amounts of glutamate (Glu) and GABA in the fresh roots were 5.720.23 and 0.280.01 mg/g dry weight (DW), respectively”

✓ located verbatim in the source · abstract, characters 167–287 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fresh radish roots contained 0.280.01 mg/g dry weight GABA.

Organ / stage
fresh rootse0
Outcome
GABAe0
Value
0.280.01 mg/g dry weighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The amounts of glutamate (Glu) and GABA in the fresh roots were 5.720.23 and 0.280.01 mg/g dry weight (DW), respectively”

✓ located verbatim in the source · abstract, characters 167–287 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sundrying increased radish GABA to 7.301.57 mg/g DW.

Treatment
sundryinge0
Outcome
GABAe0
Direction
increasede0
Value
7.301.57e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The amount of GABA by sundrying and salt-pressing process increased to 7.301.57 and 4.980.06 mg/g DW, respectively”

✓ located verbatim in the source · abstract, characters 289–403 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Salt pressing increased radish GABA to 4.980.06 mg/g DW.

Treatment
salt-pressing processe0
Outcome
GABAe0
Direction
increasede0
Value
4.980.06 mg/g DWe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The amount of GABA by sundrying and salt-pressing process increased to 7.301.57 and 4.980.06 mg/g DW, respectively”

✓ located verbatim in the source · abstract, characters 289–403 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Radish glutamate content decreased to 0.710.13 mg/g DW during processing.

Outcome
Glu contente0
Direction
decreasede0
Value
0.710.13 mg/g DWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“the Glu content decreased to 0.710.13 mg/g DW”

✓ located verbatim in the source · abstract, characters 418–463 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding MSG during salting further increased GABA in salted radish by 1.2-3.0-fold.

Treatment
addition of monosodium glutamate (MSG, 0.1-0.5 % weight of the radish)e0
Outcome
GABA content in salted radishe0
Direction
increasede0
Value
1.2-3.0-folde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The GABA content in salted radish was further increased by 1.2-3.0-fold by the addition of monosodium glutamate (MSG, 0.1-0.5 % weight of the radish) at the same time of salting”

✓ located verbatim in the source · abstract, characters 465–642 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fresh radish had the highest GAD activity, which decreased during dehydration and salting.

Treatment
dehydration and salting processe0
Outcome
GAD activitye0
Direction
decreasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“Fresh radish showed the highest GAD activity, which decreased during the dehydration and salting process”

✓ located verbatim in the source · abstract, characters 644–748 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Glu decreased over time in liquid leached from MSG-salted radishes.

Organ / stage
liquid leached from the radishese0
Outcome
Glue0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“in the liquid leached from the radishes during the salting process (agari-mizu in Japanese) with MSG, a timedependent decrease of Glu and increase of GABA were observed”

✓ located verbatim in the source · abstract, characters 769–937 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

GABA increased over time in liquid leached from MSG-salted radishes.

Organ / stage
liquid leached from the radishese0
Outcome
GABAe0
Direction
increasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“in the liquid leached from the radishes during the salting process (agari-mizu in Japanese) with MSG, a timedependent decrease of Glu and increase of GABA were observed”

✓ located verbatim in the source · abstract, characters 769–937 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggested microorganisms convert Glu to GABA in leachate, which then moves into salted radish.

Outcome
Glu was converted to GABAe0
Qualifier
by microorganisms in the agari-mizu and transferred to the salted radishe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“it was suggested that Glu was converted to GABA by microorganisms in the agari-mizu and transferred to the salted radish”

✓ located verbatim in the source · abstract, characters 945–1065 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

After two months of salting, MSG-treated radish was grayish whereas untreated radish was pale yellow.

Treatment
with MSGe0
Outcome
grayish in colore0
Qualifier
after 2 months of saltinge0
Comparator
without MSGe0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“The color of the salted radish without MSG was pale yellow, while that with MSG turned grayish in color after 2 months of salting”

✓ located verbatim in the source · abstract, characters 1067–1196 · show in text ↑
Intervention effectauthor interpretationoriginal resultnegativebasis n/a

Claim · model-written summary

The authors concluded that MSG addition during salting shortened product shelf life.

Treatment
addition of MSG during the salting processe0
Outcome
shelf-life of the producte0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Radish Roots”

✓ located verbatim in the source · title, characters 84–96 · show in text ↑
e0

“the addition of MSG during the salting process decreased the shelf-life of the product”

✓ located verbatim in the source · abstract, characters 1203–1289 · show in text ↑

salinity · molecular: metabolites · Raphanus sativus (radish)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.