Abstract
Foodborne illnesses present a major threat to public health and are frequently attributed to foodborne pathogens present on fresh produce. Some opportunistic pathogens of broccoli are also responsible for causing head rot. Three different light treatments, UV-C, red LED (50 μml/m 2 /s), and UV-C + LED were used to treat broccoli prior to or during storage. Following the light treatments, microorganisms present in eluates obtained from the surface of broccoli heads were characterized using a metagenomic approach. Metagenomic DNA libraries were subjected to high-throughput sequencing on an Illumina Hiseq platform. Results indicated that the combined treatment of LED red light and UV-C provided the best sensory preservation of broccoli, followed by LED red light and then UV-C. The bacterial communities in the eluates obtained from the surface of broccoli heads in all three light treatments were primarily represented at the phylum level by Proteobacteria and Firmicutes , while fungal communities were primarily represented by Ascomycota and Basidiomycota . Further analysis indicated that the all three light treatments reduced the presence of foodborne pathogens and bacterial taxa responsible for broccoli spoilage. While UV-C had a significant inhibitory effect on Botrytis cinerea , the light treatments increased the relative abundance of Pseudomonas fluorescens . Results indicate that a metagenomic approach can be used to detect pathogenic bacteria and fungi on fresh vegetables and assess the impact of management practices, such as light treatments, designed to maintain postharvest quality, on the composition of the microbiome present on the surface of harvested produce.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Combined LED red light and UV-C best preserved broccoli sensory quality.
- Taxon
- broccoli
Brassica oleracea var. italicat0 - Treatment
- LED red light and UV-Ce0
- Outcome
- sensory preservatione0
- Direction
- beste0
- Comparator
- LED red light and then UV-Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“combined treatment of LED red light and UV-C provided the best sensory preservation of broccoli, followed by LED red light and then UV-C”
t0“Broccoli Heads”
Claim · model-written summary
Proteobacteria and Firmicutes predominated among surface bacterial communities under all light treatments.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Interactor
- ProteobacteriaPseudomonadotae0other
- Outcome
- bacterial communitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bacterial communities in the eluates obtained from the surface of broccoli heads in all three light treatments were primarily represented at the phylum level by Proteobacteria and Firmicutes”
t0“Broccoli Heads”
Claim · model-written summary
Ascomycota and Basidiomycota predominated among surface fungal communities under all light treatments.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Interactor
- AscomycotaAscomycotae0other
- Second interactor
- BasidiomycotaBasidiomycotae0other
- Outcome
- fungal communitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fungal communities were primarily represented by Ascomycota and Basidiomycota”
t0“Broccoli Heads”
Claim · model-written summary
All three light treatments reduced foodborne pathogens on broccoli.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- all three light treatmentse0
- Outcome
- presence of foodborne pathogense0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all three light treatments reduced the presence of foodborne pathogens and bacterial taxa responsible for broccoli spoilage”
t0“Broccoli Heads”
Claim · model-written summary
All three light treatments reduced broccoli spoilage bacteria.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- all three light treatmentse0
- Outcome
- bacterial taxa responsible for broccoli spoilagee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all three light treatments reduced the presence of foodborne pathogens and bacterial taxa responsible for broccoli spoilage”
t0“Broccoli Heads”
Claim · model-written summary
UV-C significantly inhibited Botrytis cinerea.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- UV-Ce0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- Botrytis cinereae0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-C had a significant inhibitory effect on Botrytis cinerea”
t0“Broccoli Heads”
Claim · model-written summary
Light treatments increased relative abundance of Pseudomonas fluorescens.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- light treatmentse0
- Interactor
- Pseudomonas fluorescens
.JPG?width=60)
Pseudomonas fluorescense0other - Outcome
- relative abundance of Pseudomonas fluorescense0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“light treatments increased the relative abundance of Pseudomonas fluorescens”
t0“Broccoli Heads”
Claim · model-written summary
Metagenomics could detect pathogenic microbes and assess postharvest light-treatment effects on vegetable surface microbiomes.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Outcome
- detect pathogenic bacteria and fungie0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a metagenomic approach can be used to detect pathogenic bacteria and fungi on fresh vegetables and assess the impact of management practices, such as light treatments, designed to maintain postharvest quality, on the composition of the microbiome present on the surface of harvested produce”
t0“Broccoli Heads”
The same questions in other papers
light · quality: general · Brassica oleracea (wild cabbage)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- negative
6 papers more in this group
See all 10 papers →uv · quality: general · Brassica oleracea (wild cabbage)Results agree
2 papers in the same direction as this paper
- positive
- positive
light · organism · Brassica oleracea (wild cabbage)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.