Paper · 2022 · Agronomy

An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’

Juan C. Neri; Jegnes Benjamín Meléndez-Mori; José Jesús Tejada-Alvarado; et al.

5
claims
10
verbatim quotes
25
citations
3
research areas
✓
Open access · cc-by

Abstract

In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be used to cultivate strawberry plants of the ‘Aroma’ variety with high commercial quality.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 1.0% NaClO treatment for 5 min lowered explant contamination.

Taxon
StrawberryFragaria ×ananassat0
Organ / stage
explantse0
Treatment
1.0% NaClO for 5 mine0
Outcome
percentage of contaminatione0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination”

✓ located verbatim in the source · abstract, characters 372–454 · show in text ↑
t0

“Strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 66–104 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 1.0% NaClO treatment for 5 min increased explant viability, height and leaf number.

Taxon
StrawberryFragaria ×ananassat0
Organ / stage
explantse0
Treatment
1.0% NaClO for 5 mine0
Outcome
percentage of viability, height, and number of leavese0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves”

✓ located verbatim in the source · abstract, characters 372–531 · show in text ↑
t0

“Strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 66–104 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zeatin at 1 mg L−1 improved seedling shoot and root growth and leaf number.

Taxon
StrawberryFragaria ×ananassat0
Treatment
1 mg L−1 zeatine0
Outcome
number of shoots, seedling height, number of leaves, number of roots and root lengthe0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length)”

✓ located verbatim in the source · abstract, characters 568–719 · show in text ↑
t0

“Strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 66–104 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zeatin at 1 mg L−1 yielded a 100% pre-acclimation rate.

Taxon
StrawberryFragaria ×ananassat0
Treatment
1 mg L−1 zeatine0
Outcome
pre-acclimation ratee0
Direction
highere0
Value
100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%)”

✓ located verbatim in the source · abstract, characters 568–761 · show in text ↑
t0

“Strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 66–104 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Compost plus peat with 4 g humic acid improved acclimatized plant morphological and physiological responses.

Taxon
StrawberryFragaria ×ananassat0
Treatment
compost + peat combined with 4 g of humic acide0
Outcome
morphological and physiological variablese0
Direction
bettere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables”

✓ located verbatim in the source · abstract, characters 793–949 · show in text ↑
t0

“Strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 66–104 · show in text ↑

zeatin · growth · Fragaria x ananassa (strawberry)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

sodium hypochlorite · growth · Fragaria x ananassa (strawberry)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.