Abstract
Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced. The isolate Np-Bt67 produced high level of (-)-terremutin, but almost no (R)-mellein, and it was the most aggressive on grapevine, triggering apoplexy. Similar symptoms were not induced by purified (-)-terremutin. The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67. The UV9 mutant obtained from Np-B (NpB-UV9) no longer produced (-)-terremutin but overproduced (R)-mellein by 2.5-fold, and it was as pathogenic as its parent. NpB-UV9 differed from its parent by simple mutations in two genes (transcription factor UCR-NP2_6692, regulatory protein UCR-NP2_9007), not located neither near (R)-mellein, nor (-)-terremutin biosynthetic genes, but likely involved in the control of (-)-terremutin biosynthesis. Grapevine immunity was disturbed upon challenge with these pathogens or purified phytotoxins, leading to an upregulation of SA-dependent defenses, while (-)-terremutin interfered with host JA/ET-dependent defenses. Our results suggest that neither (-)-terremutin nor (R)-mellein alone is essential for the pathogenicity of N. parvum on grapevine, since isolate/mutant non-producing these toxins in vitro is pathogenic. However, these phytotoxins could play a quantitative role in the infection process.
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Claims 13
Claim · model-written summary
Isolate Np-Bt67 produced a high level of (-)-terremutin and almost no (R)-mellein.
- Material
- Np-Bt67e2
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- (-)-terremutine2
- Direction
- high levele2
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e2“The isolate Np-Bt67 produced high level of (-)-terremutin, but almost no (R)-mellein, and it was the most aggressive on grapevine, triggering apoplexy”
Claim · model-written summary
Isolate Np-Bt67 was the most aggressive on grapevine, triggering apoplexy.
- Material
- Np-Bt67e2
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- triggering apoplexye2
- Direction
- most aggressivee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e2“The isolate Np-Bt67 produced high level of (-)-terremutin, but almost no (R)-mellein, and it was the most aggressive on grapevine, triggering apoplexy”
Claim · model-written summary
Purified (-)-terremutin did not induce similar symptoms on grapevine.
- Treatment
- purified (-)-terremutine3
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- Similar symptomse3
- Direction
- not inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e2“The isolate Np-Bt67 produced high level of (-)-terremutin, but almost no (R)-mellein, and it was the most aggressive on grapevine, triggering apoplexy”
e3“Similar symptoms were not induced by purified (-)-terremutin”
Claim · model-written summary
Isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin than Np-Bt67.
- Material
- Bourgogne S-116 (Np-B)e4
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- (-)-terremutine4
- Direction
- lesse4
- Value
- 3-folde4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
Claim · model-written summary
Isolate Bourgogne S-116 (Np-B) produced high amounts of (R)-mellein.
- Material
- Bourgogne S-116 (Np-B)e4
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- (R)-melleine4
- Direction
- high amountse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
Claim · model-written summary
Isolate Bourgogne S-116 (Np-B) was less aggressive on grapevine than Np-Bt67.
- Material
- Bourgogne S-116 (Np-B)e4
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- aggressive on grapevinee4
- Direction
- less aggressivee4
- Comparator
- Np-Bt67e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
Claim · model-written summary
UV9 mutant no longer produced (-)-terremutin.
- Material
- UV9 mutante5
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- (-)-terremutine5
- Direction
- no longer producede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
e5“The UV9 mutant obtained from Np-B (NpB-UV9) no longer produced (-)-terremutin but overproduced (R)-mellein by 2.5-fold, and it was as pathogenic as its parent”
Claim · model-written summary
UV9 mutant overproduced (R)-mellein by 2.5-fold.
- Material
- UV9 mutante5
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- (R)-melleine5
- Direction
- overproducede5
- Value
- 2.5-folde5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
e5“The UV9 mutant obtained from Np-B (NpB-UV9) no longer produced (-)-terremutin but overproduced (R)-mellein by 2.5-fold, and it was as pathogenic as its parent”
Claim · model-written summary
UV9 mutant was as pathogenic on grapevine as its parent.
- Material
- UV9 mutante5
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- pathogenice5
- Direction
- as pathogenice5
- Comparator
- its parente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
e5“The UV9 mutant obtained from Np-B (NpB-UV9) no longer produced (-)-terremutin but overproduced (R)-mellein by 2.5-fold, and it was as pathogenic as its parent”
Claim · model-written summary
NpB-UV9 differed from its parent by simple mutations in two genes, one of them the transcription factor UCR-NP2_6692.
- Material
- NpB-UV9e6
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Gene
- UCR-NP2_6692e6
- Outcome
- simple mutations in two genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
e6“NpB-UV9 differed from its parent by simple mutations in two genes (transcription factor UCR-NP2_6692, regulatory protein UCR-NP2_9007)”
Claim · model-written summary
Challenge with N. parvum or purified phytotoxins upregulated SA-dependent defenses in grapevine.
- Treatment
- challenge with these pathogens or purified phytotoxinse7
- Outcome
- SA-dependent defensese7
- Direction
- upregulatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e7“Grapevine immunity was disturbed upon challenge with these pathogens or purified phytotoxins, leading to an upregulation of SA-dependent defenses, while (-)-terremutin interfered with host JA/ET-dependent defenses”
Claim · model-written summary
(-)-terremutin interfered with JA/ET-dependent defenses of grapevine.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Grapevine immunity was disturbed upon challenge with these pathogens or purified phytotoxins, leading to an upregulation of SA-dependent defenses, while (-)-terremutin interfered with host JA/ET-dependent defenses”
Claim · model-written summary
An isolate or mutant not producing these toxins in vitro is still pathogenic on grapevine.
- Interactor
- Neofusicoccum parvumNeofusicoccum parvume1pathogen
- Outcome
- pathogenice0
- Qualifier
- non-producing these toxins in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Two Neofusicoccumparvum isolates and a UV mutant were characterized for their phytotoxin production in vitro, their pathogenicity on grapevine, and their genome sequenced”
e4“The isolate Bourgogne S-116 (Np-B) produced 3-fold less (-)-terremutin and high amounts of (R)-mellein, but it was less aggressive on grapevine than Np-Bt67”
e0“since isolate/mutant non-producing these toxins in vitro is pathogenic”