Abstract
Cotton fibers, single seed trichomes derived from ovule epidermal cells, are the major source of global textile fibers. Fiber-specific promoters are desirable to study gene function and to modify fiber properties during fiber development. Here, we revealed that Rho-related GTPase6 (GhROP6) was expressed preferentially in developing fibers. A 1240 bp regulatory region of GhROP6, which contains a short upstream regulatory sequence, the first exon, and the partial first intron, was unexpectedly isolated and introduced into transgenic cotton for analyzing promoter activity. The promoter of GhROP6 (proChROP6) conferred a specific expression in ovule surface, but not in the other floral organs and vegetative tissues. Reverse transcription PCR analysis indicated that proGhROP6 directed full-length transcription of the fused ß-glucuronidase (GUS) gene. Further investigation of GUS staining showed that proChROP6 regulated gene expression in fibers and ovule epidermis from fiber initiation to cell elongation stages. The preferential activity was enriched in fiber cells after anthesis and reached to peak on flowering days. By comparison, proGhROP6 was a mild promoter with approximately one-twenty-fifth of the strength of the constitutive promoter CaMV35S. The promoter responded to high-dosage treatments of auxin, gibberellin and salicylic acid and slightly reduced GUS activity under the in vitro treatment. Collectively, our data suggest that the GhROP6 promoter has excellent activity in initiating fibers and has potential for bioengineering of cotton fibers.
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Claims 9
Claim · model-written summary
GhROP6 was preferentially expressed in developing fibers.
Verbatim
e0“Rho-related GTPase6 (GhROP6) was expressed preferentially in developing fibers”
t0“Upland Cotton”
Claim · model-written summary
The GhROP6 promoter drove specific expression on the ovule surface but not other floral or vegetative tissues.
Verbatim
e0“The promoter of GhROP6 (proChROP6) conferred a specific expression in ovule surface, but not in the other floral organs and vegetative tissues.”
t0“Upland Cotton”
Claim · model-written summary
proGhROP6 directed full-length GUS transcription.
Verbatim
e0“proGhROP6 directed full-length transcription of the fused ß-glucuronidase (GUS) gene”
t0“Upland Cotton”
Claim · model-written summary
proChROP6 regulated expression in fibers and ovule epidermis from initiation to elongation.
Verbatim
e0“proChROP6 regulated gene expression in fibers and ovule epidermis from fiber initiation to cell elongation stages”
t0“Upland Cotton”
Claim · model-written summary
Promoter activity in fiber cells peaked on flowering days.
Verbatim
e0“The preferential activity was enriched in fiber cells after anthesis and reached to peak on flowering days.”
t0“Upland Cotton”
Claim · model-written summary
proGhROP6 strength was approximately one-twenty-fifth of CaMV35S.
Verbatim
e0“proGhROP6 was a mild promoter with approximately one-twenty-fifth of the strength of the constitutive promoter CaMV35S”
t0“Upland Cotton”
Claim · model-written summary
High-dose auxin treatment slightly reduced promoter GUS activity in vitro.
Not reportedComparator
Verbatim
e0“The promoter responded to high-dosage treatments of auxin, gibberellin and salicylic acid and slightly reduced GUS activity under the in vitro treatment.”
t0“Upland Cotton”
Claim · model-written summary
High-dose gibberellin treatment slightly reduced promoter GUS activity in vitro.
Not reportedComparator
Verbatim
e0“The promoter responded to high-dosage treatments of auxin, gibberellin and salicylic acid and slightly reduced GUS activity under the in vitro treatment.”
t0“Upland Cotton”
Claim · model-written summary
High-dose salicylic acid treatment slightly reduced promoter GUS activity in vitro.
- Taxon
- Upland Cottont0
- Treatment
- salicylic acide0
- Outcome
- GUS activitye0
- Direction
- reducede0
- Setting
- in vitroe0
Not reportedComparator
Verbatim
e0“The promoter responded to high-dosage treatments of auxin, gibberellin and salicylic acid and slightly reduced GUS activity under the in vitro treatment.”
t0“Upland Cotton”
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