Abstract
Vegetable soybean is a type of value-added specialty soybean, served as a fresh vegetable or snack in China. Due to the difference from other types, it is important to understand the genetic structure and diversity of vegetable soybean for further utilization in breeding programs. The four vegetable cultivars, Taiwan-75, Zhexiandou No. 8, Zhexian No. 9 and Zhexian No. 10 are popular soybean varieties planted in Zhejiang province, and have large pods and intermediate maturity. The clustering showed a close relationship of these four cultivars in simple sequence repeat analysis. To reveal the genome variation of vegetable soybean, these four improved lines were analyzed by whole-genome re-sequencing. The average sequencing depth was 7X and the coverage ratio of each cultivar was at least more than 94%. Compared with the reference genome, a large number of single-nucleotide polymorphisms, insertion/deletions and structure variations were identified with different chromosome distributions. The average heterozygosity rate of the single-nucleotide polymorphisms was 11.99% of these four cultivars. According to the enrichment analysis, there were 23,371 genes identified with putative modifications, and a total of 282 genes were related to carbohydrate metabolic processes. These results provide useful information for genetic research and future breeding, which can facilitate the selection procedures in vegetable soybean breeding.
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Claims 7
Claim · model-written summary
SSR clustering showed a close relationship among the four cultivars.
Verbatim
e0“The clustering showed a close relationship of these four cultivars in simple sequence repeat analysis.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
Resequencing achieved average depth of 7X.
Verbatim
e0“The average sequencing depth was 7X and the coverage ratio of each cultivar was at least more than 94%.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
Each cultivar had genome coverage greater than 94%.
Verbatim
e0“The average sequencing depth was 7X and the coverage ratio of each cultivar was at least more than 94%.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
Resequencing identified many single-nucleotide polymorphisms, insertion/deletions and structural variations with differing chromosome distributions.
Verbatim
e0“Compared with the reference genome, a large number of single-nucleotide polymorphisms, insertion/deletions and structure variations were identified with different chromosome distributions.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
The four cultivars had average SNP heterozygosity of 11.99%.
Verbatim
e0“The average heterozygosity rate of the single-nucleotide polymorphisms was 11.99% of these four cultivars.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
Enrichment analysis identified 23,371 genes with putative modifications.
Verbatim
e0“According to the enrichment analysis, there were 23,371 genes identified with putative modifications, and a total of 282 genes were related to carbohydrate metabolic processes.”
t0“Vegetable Soybean Cultivars”
Claim · model-written summary
A total of 282 genes were related to carbohydrate metabolic processes.
Verbatim
e0“According to the enrichment analysis, there were 23,371 genes identified with putative modifications, and a total of 282 genes were related to carbohydrate metabolic processes.”
t0“Vegetable Soybean Cultivars”