Abstract
Mango is a popular tropical fruit that requires quarantine hot water treatment (QHWT) for postharvest sanitation, which can cause abiotic stress. Plants have various defense mechanisms to cope with stress; miRNAs mainly regulate the expression of these defense responses. Proteins involved in the biogenesis of miRNAs include DICER-like (DCL), ARGONAUTE (AGO), HYPONASTIC LEAVES 1 (HYL1), SERRATE (SE), HUA ENHANCER1 (HEN1), HASTY (HST), and HEAT-SHOCK PROTEIN 90 (HSP90), among others. According to our analysis, the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes. Gene structure prediction and domain identification indicate that sequences contain key domains for their respective gene families, including the RNase III domain in DCL and PAZ and PIWI domains for AGOs. In addition, phylogenetic analysis indicates the formation of clades that include the mango sequences and their respective orthologs in other flowering plant species, supporting the idea these are functional orthologs. The analysis of cis-regulatory elements of these genes allowed the identification of MYB, ABRE, GARE, MYC, and MeJA-responsive elements involved in stress responses. Gene expression analysis showed that most genes are induced between 3 to 6 h after QHWT, supporting the early role of miRNAs in stress response. Interestingly, our results suggest that mango rapidly induces the production of miRNAs after heat stress. This research will enable us to investigate further the regulation of gene expression and its effects on commercially cultivated fruits, such as mango, while maintaining sanitary standards.
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Claims 12
Claim · model-written summary
The mango genome contains five DCL genes.
- Taxon
- mango
_from_India.jpg?width=60)
Mangifera indicae0 - Gene
- DCLe0
- Outcome
- contains five DCLe0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_from_India.jpg?width=500)
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains thirteen AGO genes.
- Taxon
- mangoMangifera indicae0
- Gene
- AGOe0
- Outcome
- thirteen AGOe0
- Value
- thirteene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains six HYL genes.
- Taxon
- mangoMangifera indicae0
- Gene
- HYLe0
- Outcome
- six HYLe0
- Value
- sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains two SE genes.
- Taxon
- mangoMangifera indicae0
- Gene
- SEe0
- Outcome
- two SEe0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains one HEN1 gene.
- Taxon
- mangoMangifera indicae0
- Gene
- HEN1e0
- Outcome
- one HEN1e0
- Value
- onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains one HST gene.
- Taxon
- mangoMangifera indicae0
- Gene
- HSTe0
- Outcome
- one HSTe0
- Value
- onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The mango genome contains five putative HSP90 genes.
- Taxon
- mangoMangifera indicae0
- Gene
- HSP90e0
- Outcome
- five putative HSP90 genese0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes.”
Claim · model-written summary
The predicted mango gene families contain characteristic domains.
- Taxon
- mangoMangifera indicat0
- Gene
- DCL and PAZ and PIWI domains for AGOse0
- Outcome
- key domains for their respective gene familiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene structure prediction and domain identification indicate that sequences contain key domains for their respective gene families, including the RNase III domain in DCL and PAZ and PIWI domains for AGOs.”
t0“Mango”
Claim · model-written summary
Mango sequences group with orthologs in other flowering plants.
- Taxon
- mangoMangifera indicae0
- Outcome
- formation of cladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phylogenetic analysis indicates the formation of clades that include the mango sequences and their respective orthologs in other flowering plant species”
Claim · model-written summary
Stress-responsive cis elements were identified in the mango genes.
- Taxon
- mangoMangifera indicat0
- Outcome
- MYB, ABRE, GARE, MYC, and MeJA-responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The analysis of cis-regulatory elements of these genes allowed the identification of MYB, ABRE, GARE, MYC, and MeJA-responsive elements involved in stress responses.”
t0“the mango genome contains”
Claim · model-written summary
Most mango miRNA biogenesis genes were induced 3 to 6 h after quarantine hot water treatment.
- Taxon
- mangoMangifera indicat0
- Treatment
- QHWTe0
- Outcome
- Gene expressione0
- Direction
- inducede0
- Qualifier
- between 3 to 6 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene expression analysis showed that most genes are induced between 3 to 6 h after QHWT”
t0“the mango genome contains”
Claim · model-written summary
The authors infer rapid miRNA production in mango after heat stress.
- Taxon
- mangoMangifera indicae0
- Treatment
- heat stresse0
- Outcome
- production of miRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our results suggest that mango rapidly induces the production of miRNAs after heat stress”
The same questions in other papers
heat · molecular: expression · Mangifera indica (mango)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.