Abstract
MiWRKY53 is expressed in response to various stresses and hormones. Although it is localized in the nucleus, it shows no transcriptional activation. Role of SA-mediated plant defence response is demonstrated. WRKY transcription factors are one the largest gene families in plants involved in almost every process in plants including development, physiological processes, and stress response. Salicylic acid (SA) is key regulator of biotic stress against various pathogens in plants acting via its multiple mechanisms to induce defence response. Herein, we have identified and functionally validated WRKY53 from mulberry (Morus indica var. K2). MiWRKY53 expressed differentially in response to different stress and hormonal treatments. MiWRKY53 belongs to group III of WKRY gene family, localized in nucleus, and lacks transcriptional activation activity in yeast. Hormone responsive behaviour of MiWRKY53 Arabidopsis overexpression (OE) transgenics preferentially was noted in root growth assay in response to Salicylic acid (SA). Arabidopsis overexpression plants also displayed alteration in leaf phenotype having wider leaves than the wild-type plants. PR-1 transcripts were higher in MiWRKY53 Arabidopsis OE plants and they displayed resistance towards biotrophic pathogen Pseudomonas syringae PstDC3000. MiWRKY53 Mulberry OE transgenics also depicted SA-responsive behaviour. Several hormones and stress-related cis-acting elements were also identified in the 1.2-Kb upstream regulatory region (URR) of MiWRKY53. Functional characterization of full-length promoter region revealed that it is induced by SA and further analysis of deletion constructs helped in the identification of minimal promoter responsible for its inducibility by SA. Altogether, the findings from this study point towards the SA preferential behaviour of MiWRKY53 and its function as regulator of plant defence response through SA-mediated mechanisms.
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Claims 14
Claim · model-written summary
MiWRKY53 was identified and functionally validated in mulberry.
Verbatim
e0“we have identified and functionally validated WRKY53 from mulberry (Morus indica var. K2)”
Claim · model-written summary
MiWRKY53 expression differed in response to stress and hormone treatments.
- Taxon
- MiWRKY53e0
- Treatment
- different stress and hormonal treatmentse0
- Gene
- MiWRKY53e0
- Outcome
- MiWRKY53 expressede0
- Direction
- differentiallye0
Not reportedComparator
Verbatim
e0“MiWRKY53 expressed differentially in response to different stress and hormonal treatments.”
Claim · model-written summary
MiWRKY53 belongs to group III of the WRKY gene family.
Verbatim
e0“MiWRKY53 belongs to group III of WKRY gene family”
Claim · model-written summary
MiWRKY53 localized in the nucleus.
- Outcome
- localized in nucleuse0
Not reportedTaxonGene
Verbatim
e0“localized in nucleus”
Claim · model-written summary
MiWRKY53 lacked transcriptional activation activity in yeast.
Verbatim
e0“MiWRKY53 belongs to group III of WKRY gene family, localized in nucleus, and lacks transcriptional activation activity in yeast.”
Claim · model-written summary
MiWRKY53-overexpressing Arabidopsis showed a preferential root-growth response to salicylic acid.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- overexpression (OE) transgenicse0
- Organ / stage
- roote0
- Treatment
- Salicylic acid (SA)e0
- Gene
- MiWRKY53e0
- Outcome
- root growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hormone responsive behaviour of MiWRKY53 Arabidopsis overexpression (OE) transgenics preferentially was noted in root growth assay in response to Salicylic acid (SA).”
Claim · model-written summary
MiWRKY53-overexpressing Arabidopsis had wider leaves than wild-type plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- overexpression plantse0
- Organ / stage
- leafe0
- Outcome
- leaf phenotypee0
- Direction
- widere0
- Comparator
- wild-type plantse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis overexpression plants also displayed alteration in leaf phenotype having wider leaves than the wild-type plants.”
Claim · model-written summary
PR-1 transcripts were higher in MiWRKY53-overexpressing Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Material
- OE plantse0
- Gene
- PR-1e0
- Outcome
- PR-1 transcriptse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR-1 transcripts were higher in MiWRKY53 Arabidopsis OE plants”
Claim · model-written summary
MiWRKY53-overexpressing Arabidopsis resisted Pseudomonas syringae PstDC3000.
- Interactor
- Pseudomonas syringae PstDC3000
Pseudomonas syringaee0pathogen - Outcome
- resistancee0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they displayed resistance towards biotrophic pathogen Pseudomonas syringae PstDC3000.”
Claim · model-written summary
MiWRKY53-overexpressing mulberry showed a salicylic-acid response.
Verbatim
e0“MiWRKY53 Mulberry OE transgenics also depicted SA-responsive behaviour.”
Claim · model-written summary
Several hormone- and stress-related cis-acting elements were identified in the MiWRKY53 upstream regulatory region.
- Gene
- MiWRKY53e0
- Outcome
- hormones and stress-related cis-acting elementse0
- Qualifier
- 1.2-Kb upstream regulatory region (URR)e0
Not reportedTaxon
Verbatim
e0“Several hormones and stress-related cis-acting elements were also identified in the 1.2-Kb upstream regulatory region (URR) of MiWRKY53.”
Claim · model-written summary
The full-length MiWRKY53 promoter was induced by salicylic acid.
Not reportedTaxonGeneComparator
Verbatim
e0“Functional characterization of full-length promoter region revealed that it is induced by SA”
Claim · model-written summary
Analysis of deletion constructs identified the minimal promoter responsible for salicylic-acid inducibility.
Not reportedTaxonGene
Verbatim
e0“further analysis of deletion constructs helped in the identification of minimal promoter responsible for its inducibility by SA.”
Claim · model-written summary
The authors interpreted MiWRKY53 as a regulator of plant defense through salicylic-acid-mediated mechanisms.
Not reportedTaxon
Verbatim
e0“MiWRKY53 and its function as regulator of plant defence response through SA-mediated mechanisms.”