Abstract
Drought is the main abiotic stress factor limiting the growth and yield of wheat ( Triticum aestivum L.). Therefore, improving wheat tolerance to drought stress is essential for maintaining yield. Previous studies have reported on the important role of TaNRX1 in conferring drought stress tolerance. Therefore, to elucidate the regulation mechanism by which TaNRX1 confers drought resistance in wheat, we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines. The results showed that the tolerance of the OE lines to drought stress were significantly enhanced. The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT); however, the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT; the RNAi lines showed the opposite results. RNA-seq results showed that the common differentially expressed genes of TaNRX1 OE and RNAi lines, before and after drought stress, were mainly distributed in the plant–pathogen interaction, plant hormone signal transduction, phenylpropane biosynthesis, starch and sucrose metabolism, and carbon metabolism pathways and were related to the transcription factors, including WRKY, MYB, and bHLH families. This study suggests that TaNRX1 positively regulates drought stress tolerance in wheat.
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Claims 10
Claim · model-written summary
TaNRX1 overexpression significantly enhanced drought tolerance of wheat lines.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- tolerance of the OE lines to drought stresse2
- Direction
- enhancede2
- Comparator
- wild type (WT)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e2“The results showed that the tolerance of the OE lines to drought stress were significantly enhanced”
e3“the OE lines were higher than those of the wild type (WT)”
Claim · model-written summary
TaNRX1 overexpression lines had a higher survival rate under drought than wild type.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- survival ratee3
- Direction
- highere3
- Comparator
- wild type (WT)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e3“The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT)”
Claim · model-written summary
TaNRX1 overexpression lines had higher leaf chlorophyll, proline and soluble sugar content than wild type under drought.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- leaf chlorophyll, proline, soluble sugar contente3
- Direction
- highere3
- Comparator
- wild type (WT)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e3“The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT)”
Claim · model-written summary
TaNRX1 overexpression lines had higher antioxidant enzyme activities than wild type.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- activities of the antioxidant enzymese3
- Direction
- highere3
- Qualifier
- catalase, superoxide dismutase, and peroxidasee3
- Comparator
- wild type (WT)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e3“The survival rate, leaf chlorophyll, proline, soluble sugar content, and activities of the antioxidant enzymes (catalase, superoxide dismutase, and peroxidase) of the OE lines were higher than those of the wild type (WT)”
Claim · model-written summary
TaNRX1 overexpression lines had lower relative electrical conductivity and malondialdehyde than WT.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- relative electrical conductivity and malondialdehydee4
- Direction
- lowere4
- Comparator
- WTe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e4“the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT”
Claim · model-written summary
TaNRX1 overexpression lines had lower hydrogen peroxide and superoxide anion levels than WT.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- TaNRX1 overexpression (OE)e1
- Gene
- TaNRX1e1
- Outcome
- hydrogen peroxide, and superoxide anion levelse4
- Direction
- lowere4
- Comparator
- WTe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e4“the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT”
Claim · model-written summary
RNAi wheat lines showed the opposite results to TaNRX1 overexpression lines.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- RNA interference (RNAi)e1
- Gene
- TaNRX1e1
- Outcome
- the opposite resultse5
- Direction
- oppositee5
- Comparator
- WTe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e1“we generated TaNRX1 overexpression (OE) and RNA interference (RNAi) wheat lines”
e4“the relative electrical conductivity and malondialdehyde, hydrogen peroxide, and superoxide anion levels of the OE lines were lower than those of the WT”
e5“the RNAi lines showed the opposite results”
Claim · model-written summary
Common differentially expressed genes of TaNRX1 OE and RNAi lines were mainly in starch and sucrose metabolism and related pathways.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Gene
- TaNRX1e6
- Outcome
- the common differentially expressed genes of TaNRX1 OE and RNAi linese6
- Qualifier
- starch and sucrose metabolisme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e6“the common differentially expressed genes of TaNRX1 OE and RNAi lines”
e7“starch and sucrose metabolism”
Claim · model-written summary
The differentially expressed genes were related to WRKY, MYB and bHLH transcription factor families.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Gene
- WRKY, MYB, and bHLH familiese7
- Outcome
- transcription factorse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e7“were related to the transcription factors, including WRKY, MYB, and bHLH families”
Claim · model-written summary
TaNRX1 positively regulates drought stress tolerance in wheat.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Gene
- TaNRX1e7
- Outcome
- drought stress tolerancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleoredoxin Gene TaNRX1 Positively Regulates Drought Tolerance in Transgenic Wheat (Triticum aestivum L.)”
e7“This study suggests that TaNRX1 positively regulates drought stress tolerance in wheat”