Abstract
Metabolites are major contributors to the quality of tea that are regulated by various abiotic stresses. Light intensity and phosphorus (P) supply affect the metabolism of tea plants. However, how these two factors interact and mediate the metabolite levels in tea plants are not fully understood. The present study investigated the consequences of different light intensity and P regimes on the metabolism of carbohydrates, amino acids, and flavonoids in the Fengqing tea cultivar. The leaves and young shoots were subjected to untargeted metabolomics analysis by two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC–TOF/MS), ultra-performance liquid chromatography-quadrupole-TOF/MS (UPLC–Q–TOF/MS), and targeted analysis by high-performance liquid chromatography (HPLC) along with quantification of gene expression by quantitative real time-PCR (qRT–PCR). The results from young shoots showed that amino acids, pentose phosphate, and flavonol glycosides pathways were enhanced in response to decreasing light intensities and P deficiency. The expression of the genes hexokinase 1, ribose 5-phosphate isomerase A (RPIA), glutamate synthetase 1 ( GS1), prolyl 4-hydroxylase (P4H) , and arginase was induced by P limitation, thereafter affecting carbohydrates and amino acids metabolism, where shading modulated the responses of transcripts and corresponding metabolites caused by P deficiency. P deprivation repressed the expression of Pi transport, stress, sensing, and signaling (SPX2) and induced bidirectional sugar transporter (SWEET3) and amino acid permeases (AAP) which ultimately caused an increase in the amino acids: glutamate (Glu), proline (Pro), and arginine (Arg) under shading but decreased catechins [epicatechingallate (ECG) and Gallic acid, GA] content in young shoots.
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Claims 7
Claim · model-written summary
Decreasing light and phosphorus deficiency enhanced amino acid, pentose phosphate, and flavonol glycoside pathways in young shoots.
- Taxon
- Camellia sinensis L.
Camellia sinensist0 - Treatment
- decreasing light intensities and P deficiencye0
- Outcome
- amino acids, pentose phosphate, and flavonol glycosides pathwayse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amino acids, pentose phosphate, and flavonol glycosides pathways were enhanced in response to decreasing light intensities and P deficiency”
t0“Camellia sinensis L.”
Claim · model-written summary
Phosphorus limitation induced expression of hexokinase 1, RPIA, GS1, P4H, and arginase.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Treatment
- P limitatione0
- Gene
- hexokinase 1, ribose 5-phosphate isomerase A (RPIA), glutamate synthetase 1 ( GS1)e0
- Outcome
- expression of the genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of the genes hexokinase 1, ribose 5-phosphate isomerase A (RPIA), glutamate synthetase 1 ( GS1), prolyl 4-hydroxylase (P4H) , and arginase was induced by P limitation”
t0“Camellia sinensis L.”
Claim · model-written summary
Shading modulated phosphorus-deficiency responses of transcripts and corresponding metabolites.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Treatment
- shadinge0
- Outcome
- responses of transcripts and corresponding metabolitese0
- Qualifier
- P deficiencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shading modulated the responses of transcripts and corresponding metabolites caused by P deficiency”
t0“Camellia sinensis L.”
Claim · model-written summary
Phosphorus deprivation repressed SPX2 expression.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Treatment
- P deprivatione0
- Gene
- Pi transport, stress, sensing, and signaling (SPX2)e0
- Outcome
- expressione0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P deprivation repressed the expression of Pi transport, stress, sensing, and signaling (SPX2)”
t0“Camellia sinensis L.”
Claim · model-written summary
Phosphorus deprivation induced SWEET3 and AAP expression.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Treatment
- P deprivatione0
- Gene
- bidirectional sugar transporter (SWEET3) and amino acid permeases (AAP)e0
- Outcome
- bidirectional sugar transporter (SWEET3) and amino acid permeases (AAP)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P deprivation repressed the expression of Pi transport, stress, sensing, and signaling (SPX2) and induced bidirectional sugar transporter (SWEET3) and amino acid permeases (AAP)”
t0“Camellia sinensis L.”
Claim · model-written summary
Under shading, phosphorus deprivation increased glutamate, proline, and arginine in young shoots.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Treatment
- P deprivatione1
- Outcome
- amino acids: glutamate (Glu), proline (Pro), and arginine (Arg)e0
- Direction
- increasee0
- Qualifier
- under shadinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in the amino acids: glutamate (Glu), proline (Pro), and arginine (Arg) under shading”
e1“P deprivation repressed the expression of Pi transport”
t0“Camellia sinensis L.”
Claim · model-written summary
Phosphorus deprivation decreased ECG and gallic acid content in young shoots under shading.
- Taxon
- Camellia sinensis L.Camellia sinensist0
- Organ / stage
- young shootse0
- Treatment
- P deprivatione1
- Outcome
- catechins [epicatechingallate (ECG) and Gallic acid, GA] contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased catechins [epicatechingallate (ECG) and Gallic acid, GA] content in young shoots”
e1“P deprivation repressed the expression of Pi transport”
t0“Camellia sinensis L.”
The same questions in other papers
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This paper is about lacking the nutrient, so it counts the other way round here.
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.