Abstract
Jacalin-related lectins (JRLs) are a group of plant lectins with jacalin protein domain, which play important roles during plant response to biotic and abiotic stress. According their carbohydrate binding specificities, the JRLs were described as two types: galactose-binding acalins (gJRLs) and mannose-binding jacalins (mJRLs). Previous studies have shown that TaJRL53 encodes a protein with Jacalin and Dirigent domains and can be up-regulated by Fusarium graminearum infection. Wheat FHB induced by this pathogen is a disastrous disease, which not only reduces the grain yield, but also contains deoxynivalenol and other toxins, which seriously affect the health of human and livestock. To dissect the gene’s function in FHB resistance, we silenced and overexpressed the TaJRL53 in wheat respectively. As a result, attenuating TaJRL53 increased susceptibility to Fusarium graminearum. And susceptible wheat transformed with TaJRL53 over-expression showed higher Fusarium head blight resistance, the number of diseased spikelets (NDS) and length of diseased spike rachis (LDR) of transgenics wheat with TaJRL53 overexpression were significantly reduced than those of wild type. After Fusarium graminearum infection, the expression of ROS synthesis pathway, JA synthesis and signaling pathways marker genes, and disease related genes in TaJRL53 over-expression transgenic plants were higher than those in non-transgenic control. These results suggest that TaJRL53 enhanced the FHB resistance in wheat through regulating ROS synthesis pathway and JA signal transduction pathways. This study enhanced the understanding of wheat JRLs family genes’ function and laid a foundation for the mechanism dissection of TaJRL53 resistant to FHB in wheat.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Silencing TaJRL53 increased wheat susceptibility to Fusarium graminearum.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- attenuating TaJRL53e0
- Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- TaJRL53e0
- Outcome
- susceptibility to Fusarium graminearume0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“attenuating TaJRL53 increased susceptibility to Fusarium graminearum”
t0“wheat”
Claim · model-written summary
TaJRL53 overexpression raised Fusarium head blight resistance in susceptible wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TaJRL53 over-expressione0
- Interactor
- Fusarium graminearumFusarium graminearume1pathogen
- Gene
- TaJRL53e0
- Outcome
- Fusarium head blight resistancee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“susceptible wheat transformed with TaJRL53 over-expression showed higher Fusarium head blight resistance”
e1“by Fusarium graminearum infection”
Claim · model-written summary
TaJRL53 overexpression significantly reduced the number of diseased wheat spikelets relative to wild type.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TaJRL53 overexpressione0
- Interactor
- Fusarium graminearumFusarium graminearume1pathogen
- Gene
- TaJRL53e0
- Outcome
- number of diseased spikeletse0
- Direction
- reducede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of diseased spikelets (NDS) and length of diseased spike rachis (LDR) of transgenics wheat with TaJRL53 overexpression were significantly reduced than those of wild type”
e1“by Fusarium graminearum infection”
Claim · model-written summary
TaJRL53 overexpression significantly reduced the length of diseased wheat spike rachis relative to wild type.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TaJRL53 overexpressione0
- Interactor
- Fusarium graminearumFusarium graminearume1pathogen
- Gene
- TaJRL53e0
- Outcome
- length of diseased spike rachise0
- Direction
- reducede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of diseased spikelets (NDS) and length of diseased spike rachis (LDR) of transgenics wheat with TaJRL53 overexpression were significantly reduced than those of wild type”
e1“by Fusarium graminearum infection”
Claim · model-written summary
After infection, ROS synthesis pathway marker gene expression was higher in TaJRL53 overexpression wheat than in non-transgenic controls.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaJRL53 over-expressione0
- Interactor
- Fusarium graminearumFusarium graminearume0pathogen
- Gene
- TaJRL53e0
- Outcome
- expression of ROS synthesis pathwaye0
- Direction
- highere0
- Comparator
- non-transgenic controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After Fusarium graminearum infection, the expression of ROS synthesis pathway, JA synthesis and signaling pathways marker genes, and disease related genes in TaJRL53 over-expression transgenic plants were higher than those in non-transgenic control”
t0“wheat”
Claim · model-written summary
After infection, JA synthesis and signaling marker gene expression was higher in TaJRL53 overexpression wheat than in controls.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaJRL53 over-expressione0
- Interactor
- Fusarium graminearumFusarium graminearume0pathogen
- Gene
- TaJRL53e0
- Outcome
- JA synthesis and signaling pathways marker genese0
- Direction
- highere0
- Comparator
- non-transgenic controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After Fusarium graminearum infection, the expression of ROS synthesis pathway, JA synthesis and signaling pathways marker genes, and disease related genes in TaJRL53 over-expression transgenic plants were higher than those in non-transgenic control”
t0“wheat”
Claim · model-written summary
After infection, disease related gene expression was higher in TaJRL53 overexpression wheat than in controls.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaJRL53 over-expressione0
- Interactor
- Fusarium graminearumFusarium graminearume0pathogen
- Gene
- TaJRL53e0
- Outcome
- disease related genese0
- Direction
- highere0
- Comparator
- non-transgenic controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After Fusarium graminearum infection, the expression of ROS synthesis pathway, JA synthesis and signaling pathways marker genes, and disease related genes in TaJRL53 over-expression transgenic plants were higher than those in non-transgenic control”
t0“wheat”
Claim · model-written summary
The authors suggest that TaJRL53 enhances wheat head blight resistance by regulating ROS synthesis and JA signaling.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Fusarium graminearumFusarium graminearume1pathogen
- Gene
- TaJRL53e0
- Outcome
- FHB resistancee0
- Qualifier
- regulating ROS synthesis pathway and JA signal transduction pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaJRL53 enhanced the FHB resistance in wheat through regulating ROS synthesis pathway and JA signal transduction pathways”
e1“by Fusarium graminearum infection”