Abstract
The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.] is thought to be blockage of intercellular virus transport by callose deposition on plasmodesmata. But the specific regulatory mechanism remains largely unknown. In this study, we found that hydrogen peroxide (H2O2) signal downstream of NO was associated with the regulation of callose accumulation. Abundant H2O2 was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3, whereas no obvious H2O2 was observed in the compatible combination of Jidou 7 and strain SC-8. When H2O2 production was inhibited, callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination. The H2O2-associated transcriptome dynamics of soybean during SMV infection was investigated. Transcriptome and functional analysis using virus-induced gene silencing showed that GmSEOB and GmPAP27, two genes regulated by H2O2, functioned in resistance by positively regulating the accumulation of callose in response to SMV infection. These results lay a foundation for further research on the signal transduction and molecular regulation of callose deposition during soybean resistance to SMV infection.
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Claims 7
Claim · model-written summary
H2O2 signaling downstream of NO was associated with callose regulation.
- Taxon
- soybean
Glycine maxe0 - Interactor
- Soybean mosaic virus
Soybean mosaic viruse0pathogen - Compound
- hydrogen peroxide (H2O2)e1
- Outcome
- callose accumulatione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e1“hydrogen peroxide (H2O2) signal downstream of NO was associated with the regulation of callose accumulation”
Claim · model-written summary
Abundant H2O2 occurred at the cell membrane and wall in Jidou 7 with strain N3.
- Taxon
- soybeanGlycine maxe0
- Material
- Jidou 7e2
- Organ / stage
- cell membrane and cell walle2
- Interactor
- SMV strain N3e2pathogen
- Outcome
- Abundant H2O2e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e2“Abundant H2O2 was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3, whereas no obvious H2O2 was observed in the compatible combination of Jidou 7 and strain SC-8.”
Claim · model-written summary
No obvious H2O2 occurred in Jidou 7 with strain SC-8.
- Taxon
- soybeanGlycine maxe0
- Material
- Jidou 7e2
- Interactor
- strain SC-8e2pathogen
- Outcome
- no obvious H2O2e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e2“Abundant H2O2 was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3, whereas no obvious H2O2 was observed in the compatible combination of Jidou 7 and strain SC-8.”
Claim · model-written summary
Inhibiting H2O2 production decreased SMV-induced callose accumulation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- H2O2 production was inhibitede3
- Interactor
- SMVe3pathogen
- Outcome
- callose accumulatione3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e3“When H2O2 production was inhibited, callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination.”
Claim · model-written summary
After H2O2 inhibition, callose was insufficient to restrict virus transport.
- Taxon
- soybeanGlycine maxe0
- Treatment
- H2O2 production was inhibitede3
- Interactor
- SMVe3pathogen
- Outcome
- insufficient to restrict virus transporte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e3“When H2O2 production was inhibited, callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination.”
Claim · model-written summary
GmSEOB positively regulated callose accumulation during SMV infection.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SMVe4pathogen
- Gene
- GmSEOBe4
- Outcome
- accumulation of callosee4
- Direction
- positivelye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e4“GmSEOB and GmPAP27, two genes regulated by H2O2, functioned in resistance by positively regulating the accumulation of callose in response to SMV infection.”
Claim · model-written summary
GmPAP27 positively regulated callose accumulation during SMV infection.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SMVe4pathogen
- Gene
- GmPAP27e4
- Outcome
- accumulation of callosee4
- Direction
- positivelye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.]”
e4“GmSEOB and GmPAP27, two genes regulated by H2O2, functioned in resistance by positively regulating the accumulation of callose in response to SMV infection.”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.