Abstract
The present experiment was conducted to investigate the effect of chemical mutagen on biological parameters such as seed germination, shoot and root length and to fix the lethal dose (LD50) of EMS in short day Indian onion cultivar Bhima Dark Red. Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate. The optimal mutagenic doses were calculated using Probit Analysis based on germination percentage which were considered useful in developing onion mutagenized population to create genetic variability for various qualitative and quantitative traits. Seed germination was recorded after 7 and 12 days of sowing, while seedling parameters such as seedling height, dry and fresh weight of seedlings were taken after 12 days. Data revealed that the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control. Significant reduction in the seed germination percent with the enhancement of concentration of EMS dose was exhibited.
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Claims 3
Claim · model-written summary
EMS treatment significantly reduced seed germination of onion compared with control.
- Taxon
- onion
Allium cepae3 - Material
- Bhima Dark Rede3
- Treatment
- Ethyl Methane Sulfonatee1
- Outcome
- seed germinatione0
- Direction
- reducede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control”
e1“Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate”
e3“short day Indian onion cultivar Bhima Dark Red”
Claim · model-written summary
EMS treatment significantly reduced shoot and root length of onion seedlings compared with control.
- Taxon
- onion
Allium cepae3 - Material
- Bhima Dark Rede3
- Treatment
- Ethyl Methane Sulfonatee1
- Outcome
- shoot and root lengthe0
- Direction
- reducede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control”
e1“Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate”
e3“short day Indian onion cultivar Bhima Dark Red”
Claim · model-written summary
Seed germination percent of onion decreased significantly as EMS concentration increased.
- Taxon
- onion
Allium cepae3 - Treatment
- enhancement of concentration of EMS dosee2
- Outcome
- seed germination percente2
- Direction
- reductione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e2“Significant reduction in the seed germination percent with the enhancement of concentration of EMS dose was exhibited”
e3“short day Indian onion cultivar Bhima Dark Red”
The same questions in other papers
ethyl methanesulfonate · growth · Allium cepa (onion)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.