Paper · 2021 · Indian Journal of Horticulture

Optimization of EMS mutagen dose for short day onion

Hira Singh; Priyanka Verma; Sandeep Kumar Lal; et al.

3
claims
8
verbatim quotes
12
citations
1
research areas
✓
Open access

Abstract

The present experiment was conducted to investigate the effect of chemical mutagen on biological parameters such as seed germination, shoot and root length and to fix the lethal dose (LD50) of EMS in short day Indian onion cultivar Bhima Dark Red. Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate. The optimal mutagenic doses were calculated using Probit Analysis based on germination percentage which were considered useful in developing onion mutagenized population to create genetic variability for various qualitative and quantitative traits. Seed germination was recorded after 7 and 12 days of sowing, while seedling parameters such as seedling height, dry and fresh weight of seedlings were taken after 12 days. Data revealed that the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control. Significant reduction in the seed germination percent with the enhancement of concentration of EMS dose was exhibited.

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Claims 3

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

EMS treatment significantly reduced seed germination of onion compared with control.

Taxon
onionAllium cepae3
Material
Bhima Dark Rede3
Treatment
Ethyl Methane Sulfonatee1
Outcome
seed germinatione0
Direction
reducede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control”

✓ located verbatim in the source · abstract, characters 815–961 · show in text ↑
e1

“Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate”

✓ located verbatim in the source · abstract, characters 248–373 · show in text ↑
e3

“short day Indian onion cultivar Bhima Dark Red”

✓ located verbatim in the source · abstract, characters 200–246 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

EMS treatment significantly reduced shoot and root length of onion seedlings compared with control.

Taxon
onionAllium cepae3
Material
Bhima Dark Rede3
Treatment
Ethyl Methane Sulfonatee1
Outcome
shoot and root lengthe0
Direction
reducede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control”

✓ located verbatim in the source · abstract, characters 815–961 · show in text ↑
e1

“Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate”

✓ located verbatim in the source · abstract, characters 248–373 · show in text ↑
e3

“short day Indian onion cultivar Bhima Dark Red”

✓ located verbatim in the source · abstract, characters 200–246 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Seed germination percent of onion decreased significantly as EMS concentration increased.

Taxon
onionAllium cepae3
Treatment
enhancement of concentration of EMS dosee2
Outcome
seed germination percente2
Direction
reductione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“Significant reduction in the seed germination percent with the enhancement of concentration of EMS dose was exhibited”

✓ located verbatim in the source · abstract, characters 963–1080 · show in text ↑
e3

“short day Indian onion cultivar Bhima Dark Red”

✓ located verbatim in the source · abstract, characters 200–246 · show in text ↑

ethyl methanesulfonate · growth · Allium cepa (onion)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.