Abstract
. Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY-resistant tobacco.
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Claims 3
Claim · model-written summary
Va knockout produced transgene-free homozygous edited T1 tobacco plants.
- Taxon
- tobacco
Nicotiana tabacume1 - Material
- LJ911e1
- Gene
- Vae0
- Outcome
- transgene-free homozygous edited plants in the T1 generatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established”
e1“tobacco cultivar LJ911”
Claim · model-written summary
Edited LJ911 tobacco plants gained PVY resistance.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- LJ911e1
- Treatment
- Gene Va was knocked out through CRISPR/Cas9 technologye2
- Interactor
- PVY
Potato virus Ye0pathogen - Gene
- Vae2
- Outcome
- PVY resistancee0
- Direction
- gainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pathology tests indicated that the edited plants had gained PVY resistance”
e1“tobacco cultivar LJ911”
e2“Gene Va was knocked out through CRISPR/Cas9 technology”
Claim · model-written summary
The edited materials are potentially useful resources for breeding PVY-resistant tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- potentially useful genetic resources for breeding of PVY-resistant tobaccoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“edited materials generated in this study represent potentially useful genetic resources for breeding of PVY-resistant tobacco”
The same questions in other papers
loss of function · Potato virus Y · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.