Abstract
Real-time polymerase chain reaction analysis was used to compare the effect of NO-3on the activities of nitrate reductase(NR)and glutamine synthetase(GS),and the transcript levels of two NR genes,OsNia1 and OsNia2,two cytosolic GS1 genes,OsGln1;1 and OsGln1;2,and one plastid GS2 gene OsGln2,in two rice(Oryza sativa L.)cultivars Nanguang(NG)and Yunjing(YJ).Both cultivars achieved greater biomass and higher total N concentration when grown in a mixed N supply than in sole NH+ 4nutrition.Supply of NO -3increased NR activity in both leaves and roots.Expression of both NR genes was also substantially enhanced and transcript levels of OsNia2 were significantly higher than those of OsNia1.NO-3also caused an increase in GS activity,but had a complex effect on the expression of the three GS genes.In roots,the OsGln1;1 transcript increased,but OsGln1;2 decreased.In leaves,NO-3had no effect on the GS1 expression,but the transcript for OsGln2 increased both in the leaves and roots of rice with a mixed supply of N.These results suggested that the increase in GS activity might be a result of the complicated regulation of the various GS genes. In addition,the NO-3 induced increase of biomass,NR activity,GS activity,and the transcript levels of NR and GS genes were proportionally higher in NG than in YJ,indicating a stronger response of NG to NO-3nutrition than YJ.
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Claims 12
Claim · model-written summary
Nitrate supply increased nitrate reductase activity in both leaves and roots of rice.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- both leaves and rootse0
- Treatment
- NO -3e0
- Outcome
- NR activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Supply of NO -3increased NR activity in both leaves and roots”
Claim · model-written summary
Both rice cultivars achieved greater biomass and higher total N concentration with a mixed N supply than with sole NH4+ nutrition.
- Taxon
- RiceOryza sativat0
- Treatment
- mixed N supplye0
- Outcome
- biomass and higher total N concentratione0
- Direction
- greatere0
- Comparator
- sole NH+ 4nutritione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Both cultivars achieved greater biomass and higher total N concentration when grown in a mixed N supply than in sole NH+ 4nutrition”
Claim · model-written summary
Nitrate supply substantially enhanced the expression of both nitrate reductase genes in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- NO -3e0
- Gene
- NR genese0
- Outcome
- Expression of both NR genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Supply of NO -3increased NR activity in both leaves and roots.Expression of both NR genes was also substantially enhanced”
Claim · model-written summary
Transcript levels of OsNia2 were significantly higher than those of OsNia1 in rice.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“transcript levels of OsNia2 were significantly higher than those of OsNia1”
Claim · model-written summary
Nitrate supply caused an increase in glutamine synthetase activity in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- NO-3e0
- Outcome
- GS activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“NO-3also caused an increase in GS activity”
Claim · model-written summary
With nitrate supply, the OsGln1;1 transcript increased in rice roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse1
- Treatment
- NO-3e0
- Gene
- OsGln1;1e1
- Outcome
- OsGln1;1 transcripte1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“NO-3also caused an increase in GS activity”
e1“In roots,the OsGln1;1 transcript increased,but OsGln1;2 decreased”
Claim · model-written summary
With nitrate supply, the OsGln1;2 transcript decreased in rice roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse1
- Treatment
- NO-3e0
- Gene
- OsGln1;2e1
- Outcome
- OsGln1;2e1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“NO-3also caused an increase in GS activity”
e1“In roots,the OsGln1;1 transcript increased,but OsGln1;2 decreased”
Claim · model-written summary
Nitrate had no effect on GS1 expression in rice leaves.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leavese0
- Treatment
- NO-3e0
- Outcome
- GS1 expressione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“In leaves,NO-3had no effect on the GS1 expression”
Claim · model-written summary
With a mixed N supply, the OsGln2 transcript increased in both leaves and roots of rice.
- Taxon
- riceOryza sativae0
- Organ / stage
- leaves and rootse0
- Treatment
- mixed supply of Ne0
- Gene
- OsGln2e0
- Outcome
- transcript for OsGln2e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript for OsGln2 increased both in the leaves and roots of rice with a mixed supply of N”
Claim · model-written summary
The authors suggest that the increase in GS activity might result from complicated regulation of the various GS genes.
- Taxon
- RiceOryza sativat0
- Gene
- various GS genese0
- Outcome
- increase in GS activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“the increase in GS activity might be a result of the complicated regulation of the various GS genes”
Claim · model-written summary
The nitrate-induced increases in biomass, NR activity and GS activity were proportionally higher in cultivar NG than in YJ.
- Taxon
- RiceOryza sativat0
- Material
- NGe0
- Outcome
- NO-3 induced increase of biomass,NR activity,GS activitye0
- Direction
- highere0
- Comparator
- YJe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“the NO-3 induced increase of biomass,NR activity,GS activity,and the transcript levels of NR and GS genes were proportionally higher in NG than in YJ”
Claim · model-written summary
The nitrate-induced increases in transcript levels of NR and GS genes were proportionally higher in cultivar NG than in YJ.
- Taxon
- RiceOryza sativat0
- Material
- NGe0
- Outcome
- transcript levels of NR and GS genese0
- Direction
- highere0
- Comparator
- YJe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“the NO-3 induced increase of biomass,NR activity,GS activity,and the transcript levels of NR and GS genes were proportionally higher in NG than in YJ”
The same questions in other papers
nitrogen · growth · Oryza sativa (rice)Positive and negative results
401 papers in the same direction as this paper · 101 in the opposite direction · 8 reporting no difference from an alternative
- negative
- negative
- negative
507 papers more in this group
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