Abstract
β-Glucosidases are important in the formation of floral tea aroma and the development of resistance to pathogens and herbivores in tea plants.A novel β-glucosidase was purified 117-fold to homogeneity, with a yield of 1.26%.from tea leaves by chilled acetone and ammonium sulfate precipitation,ion exchange chromatography(CM-Sephadex C-50)and fast protein liquid chromatography(FPLC;Superdex 75,Resource S).The enzyme was a monomeric protein with specific activity of 2.57 U/mg.The molecular mass of the enzyme was estimated to be about 41 kDa and 34 kDa by SDS-PAGE and FPLC gel filtration on Superdex 200,respectively.The enzyme showed optimum activity at 50℃ and was stable at temperatures lower than 40℃.It was active between pH 4.0 and pH 7.0,with an optimum activity at pH 5.5,and was fairly stable from pH 4.5 to pH 8.0.The enzyme showed maximum activity towards pNPG,low activity towards pNP-Galacto,and no activity towards pNP-Xylo.
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Claims 12
Claim · model-written summary
Tea leaf β-glucosidase was purified 117-fold with 1.26% yield.
Verbatim
e0“A novel β-glucosidase was purified 117-fold to homogeneity, with a yield of 1.26%.from tea leaves”
Claim · model-written summary
The enzyme was monomeric with specific activity 2.57 U/mg.
Not reportedTaxon
Verbatim
e0“The enzyme was a monomeric protein with specific activity of 2.57 U/mg”
Claim · model-written summary
SDS-PAGE estimated enzyme molecular mass at about 41 kDa.
Not reportedTaxon
Verbatim
e0“The molecular mass of the enzyme was estimated to be about 41 kDa and 34 kDa by SDS-PAGE and FPLC gel filtration on Superdex 200,respectively”
Claim · model-written summary
FPLC gel filtration estimated enzyme molecular mass at 34 kDa.
- Gene
- enzymee0
- Outcome
- molecular mass of the enzymee0
- Value
- 34 kDae0
- Qualifier
- FPLC gel filtration on Superdex 200e0
Not reportedTaxon
Verbatim
e0“The molecular mass of the enzyme was estimated to be about 41 kDa and 34 kDa by SDS-PAGE and FPLC gel filtration on Superdex 200,respectively”
Claim · model-written summary
Enzyme activity was optimal at 50℃.
Not reportedTaxon
Verbatim
e0“The enzyme showed optimum activity at 50℃ and was stable at temperatures lower than 40℃”
Claim · model-written summary
The enzyme was stable below 40℃.
Not reportedTaxon
Verbatim
e0“The enzyme showed optimum activity at 50℃ and was stable at temperatures lower than 40℃”
Claim · model-written summary
The enzyme was active between pH 4.0 and pH 7.0.
Not reportedTaxon
Verbatim
e0“It was active between pH 4.0 and pH 7.0,with an optimum activity at pH 5.5,and was fairly stable from pH 4.5 to pH 8.0”
Claim · model-written summary
The enzyme had optimum activity at pH 5.5.
Not reportedTaxon
Verbatim
e0“It was active between pH 4.0 and pH 7.0,with an optimum activity at pH 5.5,and was fairly stable from pH 4.5 to pH 8.0”
Claim · model-written summary
The enzyme was fairly stable from pH 4.5 to pH 8.0.
Not reportedTaxon
Verbatim
e0“It was active between pH 4.0 and pH 7.0,with an optimum activity at pH 5.5,and was fairly stable from pH 4.5 to pH 8.0”
Claim · model-written summary
The enzyme showed maximum activity toward pNPG.
Not reportedTaxon
Verbatim
e0“The enzyme showed maximum activity towards pNPG,low activity towards pNP-Galacto,and no activity towards pNP-Xylo”
Claim · model-written summary
The enzyme showed low activity toward pNP-Galacto.
Not reportedTaxon
Verbatim
e0“The enzyme showed maximum activity towards pNPG,low activity towards pNP-Galacto,and no activity towards pNP-Xylo”
Claim · model-written summary
The enzyme had no activity toward pNP-Xylo.
Not reportedTaxon
Verbatim
e0“The enzyme showed maximum activity towards pNPG,low activity towards pNP-Galacto,and no activity towards pNP-Xylo”