Abstract
Cyclocarya paliurus is a medicinal,timber and ornamental tree.Farnesyl diphosphate synthase(FPS;EC2.5.1.1/EC2.5.1.10) catalyses 1′-4 condensation of the 5-carbon isoprenoid compounds isopentenyl diphosphate(IPP) and 10-carbon geranyl diphosphate(GPP) to form the 15-carbon product farnesyl diphosphate(FPP).FPP is the common precursor of sesquiterpenes,tritepenes,steroids,etc.cDNA cloning and characterization of a novel FPS from C.paliurus were described in this present study.The full-length cDNA named CpFPS(Genbank Accession Number GU121224) contained 1420bp with an open reading frame of 1029bp encoding a polypeptide of 342 amino acids with a calculated molecular mass of 39.60kDa.Through sequence analysis by BlASTP online,the resulting protein showed high homology to the FPS of Gossypium arboretum(CAA72793) and Hevea brasiliensis(BAF98301).Deduced amino acid sequence was also analyzed by biological software and the data presented the existence of conserved and functional domains of FPS.The functional analysis of CpFPS was performed by using the sterol-auxotrophic yeast strain CC25.The result indicated that the cloned cDNA of CpFPS encoding a functional FPS in C.paliurus.
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Claims 8
Claim · model-written summary
CpFPS full-length cDNA was 1420bp.
- Taxon
- Cyclocarya paliurus
Cyclocarya paliurust0 - Gene
- CpFPSe1
- Outcome
- full-length cDNAe0
- Value
- 1420bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length cDNA named CpFPS(Genbank Accession Number GU121224) contained 1420bp with an open reading frame of 1029bp”
t0“Cyclocarya paliurus”
e1“cDNA named CpFPS”
Claim · model-written summary
CpFPS open reading frame was 1029bp.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- CpFPSe1
- Outcome
- open reading framee0
- Value
- 1029bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“open reading frame of 1029bp”
t0“Cyclocarya paliurus”
e1“cDNA named CpFPS”
Claim · model-written summary
CpFPS polypeptide was 342 amino acids.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- CpFPSe1
- Outcome
- polypeptidee0
- Value
- 342 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encoding a polypeptide of 342 amino acids”
t0“Cyclocarya paliurus”
e1“cDNA named CpFPS”
Claim · model-written summary
CpFPS calculated molecular mass was 39.60kDa.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- CpFPSe1
- Outcome
- calculated molecular masse0
- Value
- 39.60kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a calculated molecular mass of 39.60kDa”
t0“Cyclocarya paliurus”
e1“cDNA named CpFPS”
Claim · model-written summary
CpFPS showed high homology to Gossypium arboretum FPS.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- FPSe0
- Outcome
- high homologye0
- Direction
- highe0
- Comparator
- Gossypium arboretume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the resulting protein showed high homology to the FPS of Gossypium arboretum(CAA72793) and Hevea brasiliensis(BAF98301)”
t0“Cyclocarya paliurus”
Claim · model-written summary
CpFPS showed high homology to Hevea brasiliensis FPS.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- FPSe0
- Outcome
- high homologye0
- Direction
- highe0
- Comparator
- Hevea brasiliensise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the resulting protein showed high homology to the FPS of Gossypium arboretum(CAA72793) and Hevea brasiliensis(BAF98301)”
t0“Cyclocarya paliurus”
Claim · model-written summary
FPS contained conserved functional domains.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- FPSe0
- Outcome
- conserved and functional domains of FPSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the data presented the existence of conserved and functional domains of FPS”
t0“Cyclocarya paliurus”
Claim · model-written summary
Cloned CpFPS encodes functional FPS.
- Taxon
- Cyclocarya paliurusCyclocarya paliurust0
- Gene
- CpFPSe0
- Outcome
- functional FPSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cloned cDNA of CpFPS encoding a functional FPS in C.paliurus”
t0“Cyclocarya paliurus”