Abstract
A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library.Sequence analysis indicated that the f128 gene was composed of 581 nt which was deduced containing an open reading frame coding for a 9.1 kD polypeptide of 85 amino acid residues(Accession No.of f128 in genBank:AY327511).The results of analysis by Biology Software showed that in the structure of the deduced protein the hydrophobic domain was dominant.It seems to have a cleavable N-terminal signal sequence, and it may entry to a secretory pathway.Genomic Southern blotting analysis indicated that f128 was one copy in genome of the millet. Northern blotting analysis showed that f128 mRNA only was accumulated in developing seeds.
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Claims 6
Claim · model-written summary
The seed-specific f128 gene was isolated from a Setaria italica cDNA library.
- Taxon
- Setaria italica
Setaria italicae0 - Organ / stage
- seede0
- Gene
- f128e0
- Outcome
- was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library.”
Claim · model-written summary
f128 comprised 581 nucleotides and encoded a predicted 85-residue, 9.1 kD polypeptide.
- Taxon
- Setaria italicaSetaria italicat0
- Gene
- f128e0
- Outcome
- gene was composede0
- Value
- 581 nte0
- Qualifier
- 9.1 kD polypeptide of 85 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the f128 gene was composed of 581 nt which was deduced containing an open reading frame coding for a 9.1 kD polypeptide of 85 amino acid residues”
t0“Setaria italica”
Claim · model-written summary
The predicted f128 protein was dominated by a hydrophobic domain.
- Taxon
- Setaria italicaSetaria italicat0
- Gene
- f128t0
- Outcome
- hydrophobic domain was dominante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the structure of the deduced protein the hydrophobic domain was dominant.”
t0“f128 Gene in Setaria italica”
Claim · model-written summary
The f128 protein appeared to have a cleavable N-terminal signal sequence and may enter a secretory pathway.
- Taxon
- Setaria italicaSetaria italicat0
- Gene
- f128t0
- Outcome
- may entry to a secretory pathwaye0
- Qualifier
- cleavable N-terminal signal sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It seems to have a cleavable N-terminal signal sequence, and it may entry to a secretory pathway.”
t0“f128 Gene in Setaria italica”
Claim · model-written summary
f128 was present as one genomic copy.
- Taxon
- Setaria italicaSetaria italicat0
- Gene
- f128e0
- Outcome
- one copy in genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genomic Southern blotting analysis indicated that f128 was one copy in genome of the millet.”
t0“Setaria italica”
Claim · model-written summary
f128 mRNA accumulated only in developing seeds.
- Taxon
- Setaria italicaSetaria italicat0
- Organ / stage
- developing seedse0
- Gene
- f128e0
- Outcome
- mRNA only was accumulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern blotting analysis showed that f128 mRNA only was accumulated in developing seeds.”
t0“Setaria italica”