Abstract
The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear. Here, widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry skin. Among them, 27 significantly differentially accumulated metabolites (DAMs) were produced in response to melatonin treatment in the presence or absence of 1-MCP. Most of the DAMs were secondary metabolites, including flavonoids, phenolic acids, stilbenes, and flavonols. Additionally, the accumulation of 25 DAMs was regulated by melatonin via ethylene. RNA-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene. Gene-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1, VvF3H, VvLAR2, and VvDFR genes, suggesting that these genes may play key roles in regulating secondary metabolites in the skin; additionally, VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites. Further experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1, thereby affecting the accumulation of secondary metabolites. Collectively, melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Metabolomics found 464 metabolites in berry skin.
Verbatim
e0“a total of 464 metabolites present in berry skin”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Melatonin treatment produced 27 significantly differentially accumulated metabolites with or without 1-MCP.
- Taxon
- grapet0
- Treatment
- melatonin treatmente0
- Outcome
- differentially accumulated metabolitese0
- Value
- 27e0
- Qualifier
- in the presence or absence of 1-MCPe0
Not reportedComparatorDirection
Verbatim
e0“27 significantly differentially accumulated metabolites (DAMs) were produced in response to melatonin treatment in the presence or absence of 1-MCP”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Most differentially accumulated metabolites were secondary metabolites.
Verbatim
e0“Most of the DAMs were secondary metabolites, including flavonoids, phenolic acids, stilbenes, and flavonols”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Melatonin regulated accumulation of 25 differentially accumulated metabolites via ethylene.
Verbatim
e0“the accumulation of 25 DAMs was regulated by melatonin via ethylene”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Melatonin primarily regulated hormone signal transduction and secondary metabolite biosynthesis pathways via ethylene.
Verbatim
e0“melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Melatonin regulated expression of VvSTS1, VvF3H, VvLAR2, and VvDFR.
Not reportedComparatorDirection
Verbatim
e0“melatonin regulated the expression of the VvSTS1, VvF3H, VvLAR2, and VvDFR genes”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
VvMYB14 and VvACS1 were implicated in secondary metabolite regulation.
Verbatim
e0“VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
Melatonin induced VvMYB14 expression.
Not reportedComparator
Verbatim
e0“melatonin induced the expression of VvMYB14”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
VvMYB14 increased ethylene production by activating VvACS1 transcription.
Verbatim
e0“VvMYB14 increased ethylene production by transcriptionally activating VvACS1”
t0“grape berry skin”
t1“grape berry skin”
Claim · model-written summary
VvMYB14 affected secondary metabolite accumulation through VvACS1 activation.
Verbatim
e0“VvMYB14 increased ethylene production by transcriptionally activating VvACS1, thereby affecting the accumulation of secondary metabolites”
t0“grape berry skin”
t1“grape berry skin”
The same questions in other papers
melatonin · molecular: expression · VitisResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.