Abstract
Exposure to ultraviolet B radiation (UV-B) stress can have serious effects on the growth and development of plants. Germin-like proteins (GLPs) may be involved in different abiotic and biotic stress responses in different plants, but little is known about the role of GLPs in UV-B stress response and acclimation in plants. In the present study, knockout of GLP 8-14 (OsGLP1) using the CRISPR/Cas9 system resulted in mutant rice (Oryza sativa L.) plants (herein called glp1) that exhibited UV-B-dependent formation of lesion mimic in leaves. Moreover, glp1 grown under solar radiation (including UV-B) showed decreased plant height and increased leaf angle, but we observed no significant differences in phenotypes between wild-type (WT) plants and glp1 grown under artificial light lacking UV-B. Fv/Fm, Y (II) and the expression of many genes, based on RNA-seq analysis, related to photosynthesis were also only reduced in glp1, but not in WT, after transfer from a growth cabinet illuminated with artificial white light lacking UV-B to growth under natural sunlight. The genes-associated with flavonoid metabolism as well as UV resistance locus 8 (OsUVR8), phytochrome interacting factor-like 15-like (OsPIF3), pyridoxal 5'-phosphate synthase subunit PDX1.2 (OsPDX1.2), deoxyribodipyrimidine photolyase (OsPHR), and deoxyribodipyrimidine photolyase family protein-like (OsPHRL) exhibited lower expression levels, while higher expression levels of mitogen-activated protein kinase 5-like (OsMPK3), mitogen-activated protein kinase 13-like (OsMPK13), and transcription factor MYB4-like (OsMYB4) were observed in glp1 than in WT after transfer from a growth cabinet illuminated with artificial white light to growth under natural sunlight. Therefore, mutations in OsGLP1 resulted in rice plants more sensitive to UV-B and reduced expression of some genes for UV-B protection, suggesting that OsGLP1 is involved in acclimation to UV-B radiation.
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Claims 9
Claim · model-written summary
OsGLP1 knockout rice formed UV-B-dependent leaf lesions.
- Taxon
- rice
Oryza sativae0 - Material
- glp1e0
- Organ / stage
- leavese0
- Treatment
- knockout of GLP 8-14 (OsGLP1)e0
- Gene
- OsGLP1e0
- Outcome
- UV-B-dependent formation of lesion mimic in leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of GLP 8-14 (OsGLP1) using the CRISPR/Cas9 system resulted in mutant rice (Oryza sativa L.) plants (herein called glp1) that exhibited UV-B-dependent formation of lesion mimic in leaves”
Claim · model-written summary
Under solar radiation, glp1 had decreased plant height.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Treatment
- solar radiation (including UV-B)e0
- Outcome
- plant heighte0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glp1 grown under solar radiation (including UV-B) showed decreased plant height and increased leaf angle”
t0“rice”
Claim · model-written summary
Under solar radiation, glp1 had increased leaf angle.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Treatment
- solar radiation (including UV-B)e0
- Outcome
- leaf anglee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glp1 grown under solar radiation (including UV-B) showed decreased plant height and increased leaf angle”
t0“rice”
Claim · model-written summary
Under artificial light lacking UV-B, glp1 and wild type had no significant phenotypic difference.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Treatment
- artificial light lacking UV-Be0
- Outcome
- phenotypese0
- Direction
- no significant differencese0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we observed no significant differences in phenotypes between wild-type (WT) plants and glp1 grown under artificial light lacking UV-B”
t0“rice”
Claim · model-written summary
Transfer to sunlight reduced Fv/Fm and Y (II) in glp1 but not wild type.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Treatment
- transfer from a growth cabinet illuminated with artificial white light lacking UV-B to growth under natural sunlighte0
- Outcome
- Fv/Fm, Y (II)e0
- Direction
- reducede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fv/Fm, Y (II) and the expression of many genes, based on RNA-seq analysis, related to photosynthesis were also only reduced in glp1, but not in WT, after transfer from a growth cabinet illuminated with artificial white light lacking UV-B to growth under natural sunlight”
t0“rice”
Claim · model-written summary
Transfer to sunlight reduced expression of many photosynthesis-related genes in glp1 but not wild type.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Treatment
- transfer from a growth cabinet illuminated with artificial white light lacking UV-B to growth under natural sunlighte0
- Outcome
- expression of many genes, based on RNA-seq analysis, related to photosynthesise0
- Direction
- reducede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of many genes, based on RNA-seq analysis, related to photosynthesis were also only reduced in glp1, but not in WT, after transfer from a growth cabinet illuminated with artificial white light lacking UV-B to growth under natural sunlight”
t0“rice”
Claim · model-written summary
After transfer to sunlight, flavonoid-metabolism genes and OsUVR8, OsPIF3, and OsPDX1.2 had lower expression in glp1 than wild type.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Gene
- OsUVR8e0
- Outcome
- expression levelse0
- Direction
- lowere0
- Qualifier
- after transfer from a growth cabinet illuminated with artificial white light to growth under natural sunlighte0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genes-associated with flavonoid metabolism as well as UV resistance locus 8 (OsUVR8), phytochrome interacting factor-like 15-like (OsPIF3), pyridoxal 5'-phosphate synthase subunit PDX1.2 (OsPDX1.2), deoxyribodipyrimidine photolyase (OsPHR), and deoxyribodipyrimidine photolyase family protein-like (OsPHRL) exhibited lower expression levels, while higher expression levels of mitogen-activated protein kinase 5-like (OsMPK3), mitogen-activated protein kinase 13-like (OsMPK13), and transcription factor MYB4-like (OsMYB4) were observed in glp1 than in WT after transfer from a growth cabinet illuminated with artificial white light to growth under natural sunlight”
t0“rice”
Claim · model-written summary
After transfer to sunlight, OsPHR and OsPHRL had lower expression in glp1 than wild type.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Gene
- OsPHRe0
- Outcome
- expression levelse0
- Direction
- lowere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deoxyribodipyrimidine photolyase (OsPHR), and deoxyribodipyrimidine photolyase family protein-like (OsPHRL) exhibited lower expression levels, while higher expression levels of mitogen-activated protein kinase 5-like (OsMPK3), mitogen-activated protein kinase 13-like (OsMPK13), and transcription factor MYB4-like (OsMYB4) were observed in glp1 than in WT after transfer from a growth cabinet illuminated with artificial white light to growth under natural sunlight”
t0“rice”
Claim · model-written summary
After transfer to sunlight, OsMPK3, OsMPK13, and OsMYB4 had higher expression in glp1 than wild type.
- Taxon
- riceOryza sativat0
- Material
- glp1e0
- Gene
- OsMPK3e0
- Outcome
- expression levelse0
- Direction
- highere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher expression levels of mitogen-activated protein kinase 5-like (OsMPK3), mitogen-activated protein kinase 13-like (OsMPK13), and transcription factor MYB4-like (OsMYB4) were observed in glp1 than in WT after transfer from a growth cabinet illuminated with artificial white light to growth under natural sunlight”
t0“rice”
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