Abstract
Date seeds (Phoenix dectylifera) are one of the seeds that is not usable and always end to be disposed. The present study describes date seeds as an additive on callus induction in vitro from hypocotyl explants of Vigna radiata. The objective is to explore the usage of date seed powder as growth additive to promote in plant tissue culture media thereby it can be utilized as fertilizer in vivo for sustainable agriculture. 1% concentrations of date seed powder under controlled conditions highly influenced the callus induction. MS media supplied with different auxins is prepared for callus induction. The highest degree of callus weight was observed in MS media supplemented with (5mg/L) 2, 4-D + (0.5 mg/L) Kn (0.437±0.1). MS media supplied with different concentrations of date seed powder + (5mg/L) 2,4-D + (0.5 mg/L) Kn are prepared for callus induction under controlled conditions (16hrs light and 8hrs dark, 3000 lux light intensity,60% humidity and 25±20c) in a plant growth chamber. MS media supplied with 1% date seed + 3% sucrose + 0.5 mg/l of Kn +5 mg/l of 2,4D gave the highest stimulation of callus growth. Results show that date seed is not replacing sucrose as a carbon source, but it acts as a good additive to promote induction callus. Quantitative nutritional analysis of date seed powder was carried out. The results show date seed powder contains a high amount of elements like: Ca (2994.33), k (1712.33), Si (456.33), Mg (687.33), which plays a major role in callus formation.
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Claims 5
Claim · model-written summary
One percent date seed powder strongly influenced callus induction.
- Taxon
- Vigna radiata
.jpg?width=60)
Vigna radiatat0 - Treatment
- 1% concentrations of date seed powdere0
- Outcome
- callus inductione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“1% concentrations of date seed powder under controlled conditions highly influenced the callus induction.”
t0“Vigna radiata”
Claim · model-written summary
The specified auxin medium produced the highest callus weight.
- Taxon
- Vigna radiataVigna radiatat0
- Treatment
- MS media supplemented with (5mg/L) 2, 4-D + (0.5 mg/L) Kne0
- Outcome
- callus weighte0
- Direction
- higheste0
- Value
- 0.437±0.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest degree of callus weight was observed in MS media supplemented with (5mg/L) 2, 4-D + (0.5 mg/L) Kn (0.437±0.1).”
t0“Vigna radiata”
Claim · model-written summary
The date seed, sucrose and growth regulator medium gave the highest callus growth stimulation.
- Taxon
- Vigna radiataVigna radiatat0
- Treatment
- 1% date seed + 3% sucrose + 0.5 mg/l of Kn +5 mg/l of 2,4De0
- Outcome
- callus growthe0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS media supplied with 1% date seed + 3% sucrose + 0.5 mg/l of Kn +5 mg/l of 2,4D gave the highest stimulation of callus growth.”
t0“Vigna radiata”
Claim · model-written summary
Date seed powder acted as an additive promoting callus induction rather than replacing sucrose.
- Taxon
- Vigna radiataVigna radiatat0
- Treatment
- date seede0
- Outcome
- promote induction calluse0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results show that date seed is not replacing sucrose as a carbon source, but it acts as a good additive to promote induction callus.”
t0“Vigna radiata”
Claim · model-written summary
Date seed powder had high measured amounts of Ca, k and Si.
Verbatim
e0“The results show date seed powder contains a high amount of elements like: Ca (2994.33), k (1712.33), Si (456.33), Mg (687.33), which plays a major role in callus formation.”
e1“Date seeds (Phoenix dectylifera)”
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