Abstract
BACKGROUND: Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level. RESULTS: To explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR. CONCLUSIONS: The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.
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Claims 7
Claim · model-written summary
Upregulated genes in Z141 were enriched in more drought-tolerance pathways than NY-17 under drought stress.
Verbatim
e0“upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS.”
t0“linseed”
Claim · model-written summary
The study identified 4436 linseed transcription factors.
Verbatim
e0“4436 linseed transcription factors were identified”
Claim · model-written summary
Of those transcription factors, 1190 responded to stress treatments.
Verbatim
e0“1190 were responsive to stress treatments.”
t0“linseed”
Claim · model-written summary
Network analysis connected proline biosynthesis with stress response genes through RIN-1.
- Taxon
- linseedt0
- Gene
- RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1)e0
- Compound
- prolinee0
- Outcome
- stress response genese0
Not reportedDirection
Verbatim
e0“the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1).”
t0“linseed”
Claim · model-written summary
RT-PCR verified expression patterns of proline biosynthesis and DNA repair structural genes.
Not reportedComparatorValue
Verbatim
e0“proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR.”
t0“linseed”
Claim · model-written summary
Authors suggest Z141 drought tolerance may relate to upregulation of drought tolerance genes.
Not reportedDirection
Verbatim
e0“The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS.”
t0“linseed”
Claim · model-written summary
Authors propose proline may aid linseed drought tolerance by maintaining osmotic balance and protecting DNA.
Verbatim
e0“Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage.”