Abstract
Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in such regulatory effects remain largely uncharacterized. To provide more insight into the mechanisms whereby temperature regulates plant-virus interactions, we analysed changes in the proteome of potato cv. Chicago plants infected with potato virus Y (PVY) at normal (22 °C) and elevated temperature (28 °C), which is known to significantly increase plant susceptibility to the virus. One of the most intriguing findings is that the main enzymes of the methionine cycle (MTC) were down-regulated at the higher but not at normal temperatures. With good agreement, we found that higher temperature conditions triggered consistent and concerted changes in the level of MTC metabolites, suggesting that the enhanced susceptibility of potato plants to PVY at 28 °C may at least be partially orchestrated by the down-regulation of MTC enzymes and concomitant cycle perturbation. In line with this, foliar treatment of these plants with methionine restored accumulation of MTC metabolites and subverted the susceptibility to PVY at elevated temperature. These data are discussed in the context of the major function of the MTC in transmethylation processes.
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Claims 6
Claim · model-written summary
Elevated temperature increased potato susceptibility to PVY.
- Taxon
- potato
Solanum tuberosume0 - Material
- cv. Chicagoe0
- Treatment
- elevated temperature (28 °C)e0
- Interactor
- potato virus Y
Potato virus Ye0pathogen - Outcome
- plant susceptibility to the viruse0
- Direction
- increasee0
- Comparator
- normal (22 °C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cv. Chicago plants infected with potato virus Y (PVY) at normal (22 °C) and elevated temperature (28 °C), which is known to significantly increase plant susceptibility to the virus”
Claim · model-written summary
At elevated but not normal temperature, main methionine cycle enzymes were downregulated in infected potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- highere0
- Interactor
- potato virus YPotato virus Yt0pathogen
- Gene
- main enzymes of the methionine cycle (MTC)e0
- Outcome
- main enzymes of the methionine cycle (MTC)e0
- Direction
- down-regulatede0
- Comparator
- normal temperaturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the main enzymes of the methionine cycle (MTC) were down-regulated at the higher but not at normal temperatures”
t0“potato plants to potato virus Y”
Claim · model-written summary
Higher temperature changed levels of methionine cycle metabolites.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- higher temperature conditionse0
- Interactor
- potato virus YPotato virus Yt0pathogen
- Outcome
- level of MTC metabolitese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher temperature conditions triggered consistent and concerted changes in the level of MTC metabolites”
t0“potato plants to potato virus Y”
Claim · model-written summary
The authors suggest methionine cycle enzyme downregulation and perturbation may partly explain susceptibility at 28 °C.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- MTC enzymese0
- Outcome
- enhanced susceptibilitye0
- Qualifier
- at 28 °Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced susceptibility of potato plants to PVY at 28 °C may at least be partially orchestrated by the down-regulation of MTC enzymes and concomitant cycle perturbation”
Claim · model-written summary
Foliar methionine restored methionine cycle metabolite accumulation at elevated temperature.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- foliar treatment of these plants with methioninee0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- accumulation of MTC metabolitese0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar treatment of these plants with methionine restored accumulation of MTC metabolites and subverted the susceptibility to PVY at elevated temperature”
t0“potato plants to potato virus Y”
Claim · model-written summary
Foliar methionine countered potato susceptibility to PVY at elevated temperature.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- foliar treatment of these plants with methioninee0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- susceptibility to PVYe0
- Direction
- subvertede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar treatment of these plants with methionine restored accumulation of MTC metabolites and subverted the susceptibility to PVY at elevated temperature”
t0“potato plants to potato virus Y”
The same questions in other papers
application · Potato virus Y · Solanum tuberosum (potato)Results agree
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