Paper · 2012 · Mağallaẗ ʻulūm al-rāfidayn

Measurement of Specific Activity of Dihydrofolate Reductase (DHFR) Enzyme and Determination of Folate Content Extracted from UV-Exposed Stem- Derived Callus of Sunflower (Helianthus annuus L.)

Sajida A. Abood; Firas H. Khathyer

7
claims
14
verbatim quotes
0
citations
1
research areas
✓
Open access · cc-by

Abstract

The results revealed to the increase of the specific activity of dihydrofolate reductase in stem' callus of sunflower, Heliathus annuus L. exposed to UV(360 nm.) for 5,10 and 15min. with the growth period. The total content of folate increased substantially, whereas 20min decreased activity of dihydrofolate reductase and folate content extracted from callus of different age. The inhibitory effect of folate analogoues aminopterin and trimethoprim, In Vivo and In Vitro on dihydrofolate reductase and folate content of sunflower callus exposed or nonexposed to UV was tested. The results revealed that the exposure of callus for five min. exhibited high level of resistance to trimethoprim and aminopterin. The specific activity of dihydrofolate reductase and the total content of folate partially reserved as a result of radiation compared with non-radiated callus

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

UV exposure for 5, 10 and 15 minutes increased dihydrofolate reductase activity with growth period.

Taxon
sunflowerHelianthus annuuse0t0
Organ / stage
stem' calluse0
Treatment
UV(360 nm.) for 5,10 and 15min.e0
Gene
dihydrofolate reductasee0
Outcome
specific activity of dihydrofolate reductasee0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“increase of the specific activity of dihydrofolate reductase in stem' callus of sunflower, Heliathus annuus L. exposed to UV(360 nm.) for 5,10 and 15min. with the growth period”

✓ located verbatim in the source · abstract, characters 28–204 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Folate content increased under the shorter UV exposures.

Taxon
sunflowerHelianthus annuuse0t0
Outcome
total content of folatee0
Direction
increasede0

Not reportedTreatmentComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The total content of folate increased substantially”

✓ located verbatim in the source · abstract, characters 206–257 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Twenty minutes of UV decreased dihydrofolate reductase activity.

Taxon
sunflowerHelianthus annuuse0t0
Treatment
20mine0
Gene
dihydrofolate reductasee0
Outcome
activity of dihydrofolate reductasee0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“20min decreased activity of dihydrofolate reductase and folate content extracted from callus of different age”

✓ located verbatim in the source · abstract, characters 267–376 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Twenty minutes of UV decreased folate content.

Taxon
sunflowerHelianthus annuuse0t0
Treatment
20mine0
Outcome
folate contente0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“20min decreased activity of dihydrofolate reductase and folate content extracted from callus of different age”

✓ located verbatim in the source · abstract, characters 267–376 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Five minutes of UV gave callus high resistance to trimethoprim.

Taxon
sunflowerHelianthus annuuse0t0
Treatment
exposure of callus for five min.e0
Outcome
resistance to trimethoprime0
Direction
high levele0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“exposure of callus for five min. exhibited high level of resistance to trimethoprim and aminopterin”

✓ located verbatim in the source · abstract, characters 608–707 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Five minutes of UV gave callus high resistance to aminopterin.

Taxon
sunflowerHelianthus annuuse0t0
Treatment
exposure of callus for five min.e0
Outcome
resistance to trimethoprim and aminopterine0
Direction
high levele0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“exposure of callus for five min. exhibited high level of resistance to trimethoprim and aminopterin”

✓ located verbatim in the source · abstract, characters 608–707 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Radiation partially preserved dihydrofolate reductase activity and folate content relative to nonradiated callus.

Taxon
sunflowerHelianthus annuuse0t0
Treatment
radiatione0
Outcome
specific activity of dihydrofolate reductase and the total content of folatee0
Direction
partially reservede0
Comparator
non-radiated calluse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The specific activity of dihydrofolate reductase and the total content of folate partially reserved as a result of radiation compared with non-radiated callus”

✓ located verbatim in the source · abstract, characters 709–867 · show in text ↑
t0

“Sunflower (Helianthus annuus L.)”

✓ located verbatim in the source · title, characters 160–192 · show in text ↑

radiation · molecular: metabolites · Helianthus annuus (common sunflower)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.