Abstract
Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase in cytoplasm (GAPCs) have remained little investigated in wheat subjected to adverse environmental conditions. In this study, we cloned and characterized a GAPC isoform TaGAPC2 in wheat. Over-expression of TaGApC2-6D in Arabidopsis led to enhanced root length, reduced reactive oxygen species (ROS) production, and elevated drought tolerance. In addition, the dual-luciferase assays showed that TaWRKY28/33/40/47 could positively regulate the expression of TaGApC2-6A and TaGApC2-6D. Further results of the yeast two-hybrid system and bimolecular fluorescence complementation assay (BiFC) demonstrate that TaPLDδ, an enzyme producing phosphatidic acid (PA), could interact with TaGAPC2-6D in plants. These results demonstrate that TaGAPC2 regulated by TaWRKY28/33/40/47 plays a crucial role in drought tolerance, which may influence the drought stress conditions via interaction with TaPLDδ. In conclusion, our results establish a new positive regulation mechanism of TaGAPC2 that helps wheat fine-tune its drought response.
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Claims 7
Claim · model-written summary
TaGAPC2 was cloned and characterized in wheat.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaGAPC2e0
- Outcome
- cloned and characterizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we cloned and characterized a GAPC isoform TaGAPC2 in wheat”
Claim · model-written summary
TaGApC2-6D overexpression increased Arabidopsis root length.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Over-expression of TaGApC2-6De0
- Gene
- TaGApC2-6De0
- Outcome
- root lengthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of TaGApC2-6D in Arabidopsis led to enhanced root length”
Claim · model-written summary
TaGApC2-6D overexpression reduced ROS production.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- reactive oxygen species (ROS) productione0
- Direction
- reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced reactive oxygen species (ROS) production”
t0“Arabidopsis Plants”
Claim · model-written summary
TaGApC2-6D overexpression elevated drought tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- drought tolerancee0
- Direction
- elevatede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated drought tolerance”
t0“Arabidopsis Plants”
Claim · model-written summary
TaWRKY28/33/40/47 positively regulated TaGApC2-6A and TaGApC2-6D expression.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaWRKY28/33/40/47e0
- Outcome
- expression of TaGApC2-6A and TaGApC2-6De0
- Direction
- positively regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaWRKY28/33/40/47 could positively regulate the expression of TaGApC2-6A and TaGApC2-6D”
e1“TaGAPC2 in wheat”
Claim · model-written summary
TaPLDδ interacted with TaGAPC2-6D in plants.
- Taxon
- plantsViridiplantaee0
- Gene
- TaPLDδe0
- Outcome
- interact with TaGAPC2-6De0
Verbatim
e0“TaPLDδ, an enzyme producing phosphatidic acid (PA), could interact with TaGAPC2-6D in plants”
Claim · model-written summary
Authors propose TaGAPC2 regulation helps wheat tune drought response.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaGAPC2e0
- Outcome
- drought responsee0
- Qualifier
- positive regulation mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a new positive regulation mechanism of TaGAPC2 that helps wheat fine-tune its drought response”