Abstract
Twenty-two genotypes of potato (Solanum tuberosum L.) were induced to form microtubers under six in vitro culture conditions. Cultures maintained under a short photoperiod (10 h of 6-12 μmol m-2 s-1) and low temperatures (day 20°±2°C and night 18°±2°C) had both a higher yield (255 mg/plantlet) and a greater number (2/plantlet) of microtubers than those maintained under long days (16 h of 38-50 μmol m-2 s-1) combined with high temperatures (day 28°±2°C and night 25°±2°C) (yield 207 mg/plantlet; microtuber number, 0.9/plantlet), over a wide range of genotypes. After the plantlets had been cultured under long days for an initial period of 60 days, continuous darkness advanced microtuberization by 2-3 months in various genotypes. Under short-day and low-temperature conditions the addition of 6-benzylaminopurine increased microtuber yield from 255 mg/plantlet to 645 mg/plantlet and average microtuber weight from 115 mg to 364 mg. A similar pattern was observed under conditions of long days and high temperature, and continuous darkness and low-temperature. Microtubers produced under light had a greater number of eyes (maximum average: 5.96/microtuber) than those produced in the dark (maximum average: 3.50/plantlet). The genotype × cultural conditions interactions were significant indicating the importance of developing genotype-specific protocols to maximize microtuberization.
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Claims 2
Claim · model-written summary
Short days and low temperature yielded more potato microtuber mass than long days and high temperature: 255 versus 207 mg per plantlet.
- Taxon
- potato
Solanum tuberosume1 - Treatment
- short photoperiod (10 h of 6-12 μmol m-2 s-1) and low temperatures (day 20°±2°C and night 18°±2°C)e0
- Outcome
- yielde0
- Direction
- highere0
- Value
- 255 mg/plantlete0
- Qualifier
- 207 mg/plantlete0
- Comparator
- long days (16 h of 38-50 μmol m-2 s-1) combined with high temperatures (day 28°±2°C and night 25°±2°C)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Cultures maintained under a short photoperiod (10 h of 6-12 μmol m-2 s-1) and low temperatures (day 20°±2°C and night 18°±2°C) had both a higher yield (255 mg/plantlet) and a greater number (2/plantlet) of microtubers than those maintained under long days (16 h of 38-50 μmol m-2 s-1) combined with high temperatures (day 28°±2°C and night 25°±2°C) (yield 207 mg/plantlet; microtuber number, 0.9/plantlet)”
e1“potato (Solanum tuberosum L.)”
Claim · model-written summary
Short days and low temperature produced more potato microtubers per plantlet than long days and high temperature: 2 versus 0.9.
- Taxon
- potato
Solanum tuberosume1 - Treatment
- short photoperiode2
- Outcome
- numbere0
- Direction
- greatere0
- Value
- 2/plantlete0
- Qualifier
- 0.9/plantlete0
- Comparator
- long dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“had both a higher yield (255 mg/plantlet) and a greater number (2/plantlet) of microtubers than those maintained under long days (16 h of 38-50 μmol m-2 s-1) combined with high temperatures (day 28°±2°C and night 25°±2°C) (yield 207 mg/plantlet; microtuber number, 0.9/plantlet)”
e1“potato (Solanum tuberosum L.)”
e2“short photoperiod (10 h of 6-12 μmol m-2 s-1) and low temperatures (day 20°±2°C and night 18°±2°C)”
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