Paper · 2020 · Archives of Phytopathology and Plant Protection

In vitroandin vivo, biocontrol activity of extracts prepared from Egyptian indigenous medicinal plants for the management of anthracnose of mango fruits

Zakaria A. M. Baka; Mohamed M. A. Mousa

10
claims
18
verbatim quotes
12
citations
3
research areas
—
Not open access

Abstract

Anthracnose is a very restrictive disease disturbing production, as well as postharvest property of mango fruit in Egypt. Fifteen isolates of Colletotrichum gloeosporioides were recovered from mango fruits displayed anthracnose symptoms. PCR assay was achieved in addition to morphological study confirmed that the causative agent of anthracnose in mango fruits is C. gloeosporioides. Anthracnose control of mango fruits by plant extracts was verified. Both ethanol and aqueous extracts prevented the growth of the pathogen in some degrees in vitro. Aqueous and ethanol extracts of Plantago sinaica were the strongest on fungal growth than other plants. A qualitative phytochemical investigation of plant extracts from testing plants designated saponins, flavonoids, alkaloids, tannins, and phenols. Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants. Analysis of phenolic compounds of Plantago sinaica extract using HPLC showed that ellagic acid represented the highest quantity than other compounds. Electron microscopy showed changes in the ultrastructure of the pathogen hyphae after treatment by Plantago sinaica extract.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

PCR and morphology confirmed C. gloeosporioides as the cause of mango anthracnose.

Organ / stage
fruitse0
Interactor
C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
Outcome
causative agent of anthracnosee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗
Colletotrichum gloeosporioides
Colletotrichum gloeosporioides · InteractorRGimilio · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“PCR assay was achieved in addition to morphological study confirmed that the causative agent of anthracnose in mango fruits is C. gloeosporioides.”

✓ located verbatim in the source · abstract, characters 238–384 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanol extracts inhibited pathogen growth in vitro.

Treatment
ethanole0
Interactor
pathogene0pathogen
Outcome
growth of the pathogene0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both ethanol and aqueous extracts prevented the growth of the pathogen in some degrees in vitro.”

✓ located verbatim in the source · abstract, characters 453–549 · show in text ↑
t0

“mango fruits”

✓ located verbatim in the source · title, characters 140–152 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Aqueous extracts inhibited pathogen growth in vitro.

Treatment
aqueous extractse0
Interactor
pathogene0pathogen
Outcome
growth of the pathogene0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both ethanol and aqueous extracts prevented the growth of the pathogen in some degrees in vitro.”

✓ located verbatim in the source · abstract, characters 453–549 · show in text ↑
t0

“mango fruits”

✓ located verbatim in the source · title, characters 140–152 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Aqueous Plantago sinaica extracts had the strongest effect on fungal growth among tested plants.

Treatment
Aqueouse0
Interactor
fungale0pathogen
Second interactor
Plantago sinaicaPlantago sinaicae0biocontrol_agent
Outcome
fungal growthe0
Direction
strongeste0
Comparator
other plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous and ethanol extracts of Plantago sinaica were the strongest on fungal growth than other plants.”

✓ located verbatim in the source · abstract, characters 550–653 · show in text ↑
t0

“mango fruits”

✓ located verbatim in the source · title, characters 140–152 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanol Plantago sinaica extracts had the strongest effect on fungal growth among tested plants.

Treatment
ethanol extracts of Plantago sinaicae0
Interactor
fungale0pathogen
Outcome
fungal growthe0
Direction
strongeste0
Comparator
other plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous and ethanol extracts of Plantago sinaica were the strongest on fungal growth than other plants.”

✓ located verbatim in the source · abstract, characters 550–653 · show in text ↑
t0

“mango fruits”

✓ located verbatim in the source · title, characters 140–152 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tested plant extracts contained saponins, flavonoids and alkaloids.

Taxon
Plantago sinaicaPlantago sinaicat0
Outcome
saponins, flavonoids, alkaloidse0

Verbatim

e0

“A qualitative phytochemical investigation of plant extracts from testing plants designated saponins, flavonoids, alkaloids, tannins, and phenols.”

✓ located verbatim in the source · abstract, characters 654–799 · show in text ↑
t0

“Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants.”

✓ located verbatim in the source · abstract, characters 800–891 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tested plant extracts contained tannins and phenols.

Taxon
Plantago sinaicaPlantago sinaicat0
Outcome
tannins, and phenolse0

Verbatim

e0

“A qualitative phytochemical investigation of plant extracts from testing plants designated saponins, flavonoids, alkaloids, tannins, and phenols.”

✓ located verbatim in the source · abstract, characters 654–799 · show in text ↑
t0

“Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants.”

✓ located verbatim in the source · abstract, characters 800–891 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Plantago sinaica extract had more phenolic compounds than other plants.

Taxon
Plantago sinaicaPlantago sinaicae0
Outcome
amount of phenolic compoundse0
Direction
highere0
Comparator
other plantse0

Verbatim

e0

“Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants.”

✓ located verbatim in the source · abstract, characters 800–891 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ellagic acid was the most abundant phenolic compound in Plantago sinaica extract.

Taxon
Plantago sinaicaPlantago sinaicat0
Outcome
ellagic acid represented the highest quantitye0
Direction
higheste0
Comparator
other compoundse0

Verbatim

e0

“ellagic acid represented the highest quantity than other compounds.”

✓ located verbatim in the source · abstract, characters 974–1041 · show in text ↑
t0

“Plantago sinaica extract exhibited a higher amount of phenolic compounds than other plants.”

✓ located verbatim in the source · abstract, characters 800–891 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Plantago sinaica extract changed pathogen hyphal ultrastructure.

Treatment
Plantago sinaica extracte0
Interactor
pathogene0pathogen
Outcome
ultrastructure of the pathogen hyphaee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Electron microscopy showed changes in the ultrastructure of the pathogen hyphae after treatment by Plantago sinaica extract.”

✓ located verbatim in the source · abstract, characters 1042–1166 · show in text ↑
t0

“mango fruits”

✓ located verbatim in the source · title, characters 140–152 · show in text ↑

botanical · growth · Mangifera indica (mango)Results agree

14 papers: 14 positive, 0 negative or no effect

13 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.