Abstract
The thylakoid membrane is a highly complex membrane system in plants and plays crucial roles in the biogenesis of the photosynthetic apparatus and plant development. However, the genetic factors involved in chloroplast development and its relationship with intracellular metabolites are largely unknown. Here, a rice (Oryza sativa) chlorotic and necrotic leaf1 (cnl1) mutant was identified and map-based cloning revealed that a single base substitution followed by a 6-bp deletion in the ATP-binding cassette transporter I family member7 (OsABCI7) resulted in chlorotic and necrotic leaves with thylakoid membrane degradation, chlorophyll breakdown, photosynthesis impairment, and cell death in cnl1. Furthermore, the expression of OsABCI7 was inducible under lower temperatures, which severely affected cnl1 chloroplast development, and etiolated cnl1 seedlings were unable to recover to a normal green state under light conditions. Functional complementation and overexpression showed that OsABCI7 could rescue the cnl1 chlorotic and necrotic phenotype. OsABCI7 interacted with HIGH CHLOROPHYLL FLUORESCENCE222 (OsHCF222) to regulate cellular reactive oxygen species (ROS) homeostasis for thylakoid membrane stability. OsABCI7 localized to thylakoid membranes, while OsHCF222 targeted to endoplasmic reticulum and chloroplasts. Exogenous application of ascorbic acid eased the yellowish leaf phenotype by increasing chlorophyll content and alleviating ROS stress in cnl1. Unlike cnl1, the CRISPR/Cas9-mediated OsHCF222 knockout lines showed chlorotic leaves but were seedling lethal. Our results provide insight into the functions of ABC transporters in rice, especially within the relationship between ROS homeostasis and stability of thylakoid membranes.
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Claims 15
Claim · model-written summary
Map-based cloning of the rice cnl1 mutant revealed a single base substitution followed by a 6-bp deletion in OsABCI7.
- Taxon
- Rice
Oryza sativat0 - Material
- cnl1e2
- Gene
- OsABCI7e1
- Outcome
- single base substitution followed by a 6-bp deletione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“map-based cloning revealed that a single base substitution followed by a 6-bp deletion in the ATP-binding cassette transporter I family member7 (OsABCI7)”
e2“a rice (Oryza sativa) chlorotic and necrotic leaf1 (cnl1) mutant was identified”
Claim · model-written summary
The cnl1 mutant showed chlorotic and necrotic leaves.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Outcome
- chlorotic and necrotic leavese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“resulted in chlorotic and necrotic leaves with thylakoid membrane degradation, chlorophyll breakdown, photosynthesis impairment, and cell death in cnl1”
Claim · model-written summary
The cnl1 mutant showed thylakoid membrane degradation, chlorophyll breakdown and photosynthesis impairment.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Outcome
- thylakoid membrane degradation, chlorophyll breakdown, photosynthesis impairmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“resulted in chlorotic and necrotic leaves with thylakoid membrane degradation, chlorophyll breakdown, photosynthesis impairment, and cell death in cnl1”
Claim · model-written summary
The cnl1 mutant showed cell death.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Outcome
- cell deathe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“resulted in chlorotic and necrotic leaves with thylakoid membrane degradation, chlorophyll breakdown, photosynthesis impairment, and cell death in cnl1”
Claim · model-written summary
OsABCI7 expression was inducible under lower temperatures.
- Taxon
- RiceOryza sativat0
- Treatment
- lower temperaturese1
- Gene
- OsABCI7e1
- Outcome
- expression of OsABCI7e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“the expression of OsABCI7 was inducible under lower temperatures”
Claim · model-written summary
Lower temperatures severely affected chloroplast development in cnl1.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Treatment
- lower temperaturese1
- Outcome
- chloroplast developmente1
- Qualifier
- severely affectede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“the expression of OsABCI7 was inducible under lower temperatures, which severely affected cnl1 chloroplast development”
Claim · model-written summary
Etiolated cnl1 seedlings could not recover to a normal green state under light conditions.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Treatment
- light conditionse1
- Outcome
- unable to recover to a normal green statee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“etiolated cnl1 seedlings were unable to recover to a normal green state under light conditions”
Claim · model-written summary
Functional complementation and overexpression of OsABCI7 rescued the cnl1 chlorotic and necrotic phenotype.
- Taxon
- RiceOryza sativat0
- Treatment
- Functional complementation and overexpressione1
- Gene
- OsABCI7e1
- Outcome
- cnl1 chlorotic and necrotic phenotypee1
- Direction
- rescuee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“Functional complementation and overexpression showed that OsABCI7 could rescue the cnl1 chlorotic and necrotic phenotype”
Claim · model-written summary
OsABCI7 interacted with OsHCF222 to regulate cellular ROS homeostasis for thylakoid membrane stability.
- Taxon
- RiceOryza sativat0
- Gene
- OsABCI7e1
- Outcome
- thylakoid membrane stabilitye1
- Qualifier
- regulate cellular reactive oxygen species (ROS) homeostasise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“OsABCI7 interacted with HIGH CHLOROPHYLL FLUORESCENCE222 (OsHCF222) to regulate cellular reactive oxygen species (ROS) homeostasis for thylakoid membrane stability”
Claim · model-written summary
OsABCI7 localized to thylakoid membranes.
- Taxon
- RiceOryza sativat0
- Gene
- OsABCI7e1
- Outcome
- localized to thylakoid membranese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“OsABCI7 localized to thylakoid membranes”
Claim · model-written summary
OsHCF222 targeted to the endoplasmic reticulum and chloroplasts.
- Taxon
- RiceOryza sativat0
- Gene
- OsHCF222e1
- Outcome
- targeted to endoplasmic reticulum and chloroplastse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“OsHCF222 targeted to endoplasmic reticulum and chloroplasts”
Claim · model-written summary
Exogenous ascorbic acid eased the yellowish leaf phenotype of cnl1.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Treatment
- Exogenous application of ascorbic acide1
- Outcome
- yellowish leaf phenotypee1
- Direction
- easede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“Exogenous application of ascorbic acid eased the yellowish leaf phenotype by increasing chlorophyll content and alleviating ROS stress in cnl1”
Claim · model-written summary
Exogenous ascorbic acid increased chlorophyll content in cnl1.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Treatment
- Exogenous application of ascorbic acide1
- Outcome
- chlorophyll contente1
- Direction
- increasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“Exogenous application of ascorbic acid eased the yellowish leaf phenotype by increasing chlorophyll content and alleviating ROS stress in cnl1”
Claim · model-written summary
Exogenous ascorbic acid alleviated ROS stress in cnl1.
- Taxon
- RiceOryza sativat0
- Material
- cnl1e1
- Treatment
- Exogenous application of ascorbic acide1
- Outcome
- ROS stresse1
- Direction
- alleviatinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“Exogenous application of ascorbic acid eased the yellowish leaf phenotype by increasing chlorophyll content and alleviating ROS stress in cnl1”
Claim · model-written summary
CRISPR/Cas9-mediated OsHCF222 knockout lines showed chlorotic leaves and were seedling lethal, unlike cnl1.
- Taxon
- RiceOryza sativat0
- Treatment
- CRISPR/Cas9-mediated OsHCF222 knockoute1
- Gene
- OsHCF222e1
- Outcome
- chlorotic leaves but were seedling lethale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e1“Unlike cnl1, the CRISPR/Cas9-mediated OsHCF222 knockout lines showed chlorotic leaves but were seedling lethal”
The same questions in other papers
ascorbic acid · physiology: pigments · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.